HIF – 1α as a marker of risk assessment for malignant transformation in oral submucous fibrosis: An immunohistochemical study
Background: Oral submucous fibrosis (OSMF) is a potentially malignant disorder associated with habit of chewing betel quid containing arecanut. Morphological features of OSMF especially fibrosis suggests a possibility of the hypoxic environment in diseased tissues. The adaptation of cells to hypoxia...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Indian Journal of Pathology and Microbiology |
| Subjects: | |
| Online Access: | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2022;volume=65;issue=4;spage=761;epage=765;aulast=Sharma |
| _version_ | 1797963060877459456 |
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| author | Kanika Sharma Devi C Shetty Ajit S Rathore Saurabh A Juneja Sonal Katyal Ankaj Krishnatre |
| author_facet | Kanika Sharma Devi C Shetty Ajit S Rathore Saurabh A Juneja Sonal Katyal Ankaj Krishnatre |
| author_sort | Kanika Sharma |
| collection | DOAJ |
| description | Background: Oral submucous fibrosis (OSMF) is a potentially malignant disorder associated with habit of chewing betel quid containing arecanut. Morphological features of OSMF especially fibrosis suggests a possibility of the hypoxic environment in diseased tissues. The adaptation of cells to hypoxia appears to be mediated via hypoxia inducible factor-1α (HIF-1α) which is also said to be associated with malignant transformation of epithelial cells in various other carcinomas like prostate and cervical carcinoma. Therefore, the main objective of this study was to investigate the role of HIF-1α in progression and malignant transformation of OSMF. Materials and Methods: The study group consisted of histo-pathologically diagnosed 30 cases of oral submucous fibrosis and 10 cases of OSCC were taken as control. The immunohistochemistry was carried out on neutral buffered formalin-fixed paraffin-embedded tissue sections by using the monoclonal antibody of HIF-1α. Statistical analysis was done using SPSS software version 2.0. Results: A gradual and significant rise in the expression of HIF-1α was observed in various grades of OSMF and OSCC cases. HIF 1α expression was increased in cases showing hylanization and constricted blood vessels. A cut off value of 39.6% of HIF-1α positive cells was determined statistically to categorize the cases into high risk and low risk group for malignant transformation. Conclusion: Overexpression of HIF-1α may contribute to the progression and malignant transformation of OSMF. Cases expressing more than 40% of HIF-1α positive cells are at a greater risk for malignant transformation. |
| first_indexed | 2024-04-11T01:23:31Z |
| format | Article |
| id | doaj.art-1fea079b83b7412d84e9ef90eab7d828 |
| institution | Directory Open Access Journal |
| issn | 0377-4929 |
| language | English |
| last_indexed | 2024-04-11T01:23:31Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Pathology and Microbiology |
| spelling | doaj.art-1fea079b83b7412d84e9ef90eab7d8282023-01-03T10:48:41ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49292022-01-0165476176510.4103/ijpm.ijpm_160_21HIF – 1α as a marker of risk assessment for malignant transformation in oral submucous fibrosis: An immunohistochemical studyKanika SharmaDevi C ShettyAjit S RathoreSaurabh A JunejaSonal KatyalAnkaj KrishnatreBackground: Oral submucous fibrosis (OSMF) is a potentially malignant disorder associated with habit of chewing betel quid containing arecanut. Morphological features of OSMF especially fibrosis suggests a possibility of the hypoxic environment in diseased tissues. The adaptation of cells to hypoxia appears to be mediated via hypoxia inducible factor-1α (HIF-1α) which is also said to be associated with malignant transformation of epithelial cells in various other carcinomas like prostate and cervical carcinoma. Therefore, the main objective of this study was to investigate the role of HIF-1α in progression and malignant transformation of OSMF. Materials and Methods: The study group consisted of histo-pathologically diagnosed 30 cases of oral submucous fibrosis and 10 cases of OSCC were taken as control. The immunohistochemistry was carried out on neutral buffered formalin-fixed paraffin-embedded tissue sections by using the monoclonal antibody of HIF-1α. Statistical analysis was done using SPSS software version 2.0. Results: A gradual and significant rise in the expression of HIF-1α was observed in various grades of OSMF and OSCC cases. HIF 1α expression was increased in cases showing hylanization and constricted blood vessels. A cut off value of 39.6% of HIF-1α positive cells was determined statistically to categorize the cases into high risk and low risk group for malignant transformation. Conclusion: Overexpression of HIF-1α may contribute to the progression and malignant transformation of OSMF. Cases expressing more than 40% of HIF-1α positive cells are at a greater risk for malignant transformation.http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2022;volume=65;issue=4;spage=761;epage=765;aulast=Sharmahifhigh risk caseshypoxiamalignant transformationoral submucous fibrosis. |
| spellingShingle | Kanika Sharma Devi C Shetty Ajit S Rathore Saurabh A Juneja Sonal Katyal Ankaj Krishnatre HIF – 1α as a marker of risk assessment for malignant transformation in oral submucous fibrosis: An immunohistochemical study Indian Journal of Pathology and Microbiology hif high risk cases hypoxia malignant transformation oral submucous fibrosis. |
| title | HIF – 1α as a marker of risk assessment for malignant transformation in oral submucous fibrosis: An immunohistochemical study |
| title_full | HIF – 1α as a marker of risk assessment for malignant transformation in oral submucous fibrosis: An immunohistochemical study |
| title_fullStr | HIF – 1α as a marker of risk assessment for malignant transformation in oral submucous fibrosis: An immunohistochemical study |
| title_full_unstemmed | HIF – 1α as a marker of risk assessment for malignant transformation in oral submucous fibrosis: An immunohistochemical study |
| title_short | HIF – 1α as a marker of risk assessment for malignant transformation in oral submucous fibrosis: An immunohistochemical study |
| title_sort | hif 1α as a marker of risk assessment for malignant transformation in oral submucous fibrosis an immunohistochemical study |
| topic | hif high risk cases hypoxia malignant transformation oral submucous fibrosis. |
| url | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2022;volume=65;issue=4;spage=761;epage=765;aulast=Sharma |
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