Experimental protocol for metabolic acidosis induction by intravenous administration of hydrochloric acid in sheep
ABSTRACT The aim of this study was to assess the magnitude and duration of blood and urine changes and the side effects of hyperchloremic acidosis induced by the intravenous administration of hydrochloric acid in sheep. Five healthy, crossbred adult ewes, with a mean body weight of 44±2.9kg were use...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100053&lng=en&tlng=en |
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| author | F.T.N.M.A. Romão J.M. Curti P.F.V. Pereira K.K.M.C. Flaiban J.A.N. Lisbôa |
| author_facet | F.T.N.M.A. Romão J.M. Curti P.F.V. Pereira K.K.M.C. Flaiban J.A.N. Lisbôa |
| author_sort | F.T.N.M.A. Romão |
| collection | DOAJ |
| description | ABSTRACT The aim of this study was to assess the magnitude and duration of blood and urine changes and the side effects of hyperchloremic acidosis induced by the intravenous administration of hydrochloric acid in sheep. Five healthy, crossbred adult ewes, with a mean body weight of 44±2.9kg were used. The hydrochloric acid solution was administered intravenously at a rate of 25mL/kg/h for 4 hours continuously. Venous blood and urine samples were collected and pH values, blood carbon dioxide partial pressure, bicarbonate, base excess, strong ion difference, anion gap, total concentration of nonvolatile buffers, creatinine, plasma L-lactate, plasma and urine sodium, potassium, and chloride were determined. The experimental protocol induced severe hyperchloremic acidosis at the end of the infusion, with a decreased plasma strong ion difference. The fractional excretion of sodium and chloride remained increased during 4 hours after the infusion. Aciduria was observed at approximately 24 hours. Twenty-four hours after the infusion, the animals showed mild and compensated metabolic acidosis. This protocol was effective in inducing severe and long-lasting hyperchloremic acidosis and did not cause serious side effects. Therefore, this protocol can be used safely in adult sheep for studies on the treatment of this condition. |
| first_indexed | 2024-12-21T11:25:51Z |
| format | Article |
| id | doaj.art-1feb214e7a984d1b8ca9710f241f46aa |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-12-21T11:25:51Z |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-1feb214e7a984d1b8ca9710f241f46aa2022-12-21T19:05:40ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-4162711536010.1590/1678-4162-10106S0102-09352019000100053Experimental protocol for metabolic acidosis induction by intravenous administration of hydrochloric acid in sheepF.T.N.M.A. RomãoJ.M. CurtiP.F.V. PereiraK.K.M.C. FlaibanJ.A.N. LisbôaABSTRACT The aim of this study was to assess the magnitude and duration of blood and urine changes and the side effects of hyperchloremic acidosis induced by the intravenous administration of hydrochloric acid in sheep. Five healthy, crossbred adult ewes, with a mean body weight of 44±2.9kg were used. The hydrochloric acid solution was administered intravenously at a rate of 25mL/kg/h for 4 hours continuously. Venous blood and urine samples were collected and pH values, blood carbon dioxide partial pressure, bicarbonate, base excess, strong ion difference, anion gap, total concentration of nonvolatile buffers, creatinine, plasma L-lactate, plasma and urine sodium, potassium, and chloride were determined. The experimental protocol induced severe hyperchloremic acidosis at the end of the infusion, with a decreased plasma strong ion difference. The fractional excretion of sodium and chloride remained increased during 4 hours after the infusion. Aciduria was observed at approximately 24 hours. Twenty-four hours after the infusion, the animals showed mild and compensated metabolic acidosis. This protocol was effective in inducing severe and long-lasting hyperchloremic acidosis and did not cause serious side effects. Therefore, this protocol can be used safely in adult sheep for studies on the treatment of this condition.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100053&lng=en&tlng=enequilíbrio ácido-baseacidose hiperclorêmicaruminanteacidose por íons fortes |
| spellingShingle | F.T.N.M.A. Romão J.M. Curti P.F.V. Pereira K.K.M.C. Flaiban J.A.N. Lisbôa Experimental protocol for metabolic acidosis induction by intravenous administration of hydrochloric acid in sheep Arquivo Brasileiro de Medicina Veterinária e Zootecnia equilíbrio ácido-base acidose hiperclorêmica ruminante acidose por íons fortes |
| title | Experimental protocol for metabolic acidosis induction by intravenous administration of hydrochloric acid in sheep |
| title_full | Experimental protocol for metabolic acidosis induction by intravenous administration of hydrochloric acid in sheep |
| title_fullStr | Experimental protocol for metabolic acidosis induction by intravenous administration of hydrochloric acid in sheep |
| title_full_unstemmed | Experimental protocol for metabolic acidosis induction by intravenous administration of hydrochloric acid in sheep |
| title_short | Experimental protocol for metabolic acidosis induction by intravenous administration of hydrochloric acid in sheep |
| title_sort | experimental protocol for metabolic acidosis induction by intravenous administration of hydrochloric acid in sheep |
| topic | equilíbrio ácido-base acidose hiperclorêmica ruminante acidose por íons fortes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100053&lng=en&tlng=en |
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