The phylogenetic authentication of Ophiocordyceps sphecocephala from Lang Biang Biosphere Reserve, Lam Dong, Vietnam
During our expedition at Lang Biang Biosphere Reserve, samples of DL0023, parasitizing on wasps, were collected, then sent to the biology laboratory, Dalat University, Lam Dong Province, Vietnam, to preliminarily morphologically identify the name of this species as Ophiocordyceps sphecocephala. The...
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HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE
2023-04-01
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| Series: | Ho Chi Minh City Open University Journal of Science - Engineering and Technology |
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| Online Access: | https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/2641 |
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| author | Lao Duc Thuan Pham Thi Dinh Thieu Hong Hue Vu Hoang Hai Nguyen Van Giang Truong Binh Nguyen Le Huyen Ai Thuy |
| author_facet | Lao Duc Thuan Pham Thi Dinh Thieu Hong Hue Vu Hoang Hai Nguyen Van Giang Truong Binh Nguyen Le Huyen Ai Thuy |
| author_sort | Lao Duc Thuan |
| collection | DOAJ |
| description | During our expedition at Lang Biang Biosphere Reserve, samples of DL0023, parasitizing on wasps, were collected, then sent to the biology laboratory, Dalat University, Lam Dong Province, Vietnam, to preliminarily morphologically identify the name of this species as Ophiocordyceps sphecocephala. The method of Phenol (pH = 8)/Chloroform method was applied to isolated DNA. The PCR assays and sequencing were performed to amplify and sequencing ITS, nrLSU, and nrSSU, respectively. The phylogenetic methods of neighbor-joining, maximum likelihood, and maximum parsimony were constructed to identify the relationship between DL0023 and referent sequences. We successfully amplified and sequenced the target genes, and constructed concatenated data containing 29, 33, and 30 sequences of ITS, nrLSU and nrSSU, respectively, belonging to Clavicipitaceae clade A, clade B and clade C, and 02 sequences belonged to Glomerellaceae. The results of phylogenetic analysis shown that DL0023 was accepted at Ophiocordyceps and strongly formed the monophyletic group Ophiocordyceps sphecocephala with a high supported value. In conclusion, the concatenated dataset’s phylogenetic analyses effectively supported the identification of DL0023 as Ophiocordyceps sphecocephala. |
| first_indexed | 2024-04-09T15:41:38Z |
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| id | doaj.art-1feb9f0df4624e12a5a718131ad987b1 |
| institution | Directory Open Access Journal |
| issn | 2734-9330 2734-9608 |
| language | English |
| last_indexed | 2024-04-09T15:41:38Z |
| publishDate | 2023-04-01 |
| publisher | HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE |
| record_format | Article |
| series | Ho Chi Minh City Open University Journal of Science - Engineering and Technology |
| spelling | doaj.art-1feb9f0df4624e12a5a718131ad987b12023-04-27T06:54:46ZengHO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCEHo Chi Minh City Open University Journal of Science - Engineering and Technology2734-93302734-96082023-04-01131182310.46223/HCMCOUJS.tech.en.13.1.2641.20231879The phylogenetic authentication of Ophiocordyceps sphecocephala from Lang Biang Biosphere Reserve, Lam Dong, VietnamLao Duc Thuan0Pham Thi Dinh1Thieu Hong Hue2Vu Hoang Hai3Nguyen Van Giang4Truong Binh Nguyen5Le Huyen Ai Thuy6Ho Chi Minh City Open University, Ho Chi Minh CityHo Chi Minh City Open University, Ho Chi Minh CityHo Chi Minh City Open University, Ho Chi Minh CityHo Chi Minh City Open University, Ho Chi Minh CityDalat University, Dalat CityDalat University, Dalat CityHo Chi Minh City Open University, Ho Chi Minh CityDuring our expedition at Lang Biang Biosphere Reserve, samples of DL0023, parasitizing on wasps, were collected, then sent to the biology laboratory, Dalat University, Lam Dong Province, Vietnam, to preliminarily morphologically identify the name of this species as Ophiocordyceps sphecocephala. The method of Phenol (pH = 8)/Chloroform method was applied to isolated DNA. The PCR assays and sequencing were performed to amplify and sequencing ITS, nrLSU, and nrSSU, respectively. The phylogenetic methods of neighbor-joining, maximum likelihood, and maximum parsimony were constructed to identify the relationship between DL0023 and referent sequences. We successfully amplified and sequenced the target genes, and constructed concatenated data containing 29, 33, and 30 sequences of ITS, nrLSU and nrSSU, respectively, belonging to Clavicipitaceae clade A, clade B and clade C, and 02 sequences belonged to Glomerellaceae. The results of phylogenetic analysis shown that DL0023 was accepted at Ophiocordyceps and strongly formed the monophyletic group Ophiocordyceps sphecocephala with a high supported value. In conclusion, the concatenated dataset’s phylogenetic analyses effectively supported the identification of DL0023 as Ophiocordyceps sphecocephala.https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/2641entomopathogenic fungilang biang biosphere reserveophiocordycepsphylogeny |
| spellingShingle | Lao Duc Thuan Pham Thi Dinh Thieu Hong Hue Vu Hoang Hai Nguyen Van Giang Truong Binh Nguyen Le Huyen Ai Thuy The phylogenetic authentication of Ophiocordyceps sphecocephala from Lang Biang Biosphere Reserve, Lam Dong, Vietnam Ho Chi Minh City Open University Journal of Science - Engineering and Technology entomopathogenic fungi lang biang biosphere reserve ophiocordyceps phylogeny |
| title | The phylogenetic authentication of Ophiocordyceps sphecocephala from Lang Biang Biosphere Reserve, Lam Dong, Vietnam |
| title_full | The phylogenetic authentication of Ophiocordyceps sphecocephala from Lang Biang Biosphere Reserve, Lam Dong, Vietnam |
| title_fullStr | The phylogenetic authentication of Ophiocordyceps sphecocephala from Lang Biang Biosphere Reserve, Lam Dong, Vietnam |
| title_full_unstemmed | The phylogenetic authentication of Ophiocordyceps sphecocephala from Lang Biang Biosphere Reserve, Lam Dong, Vietnam |
| title_short | The phylogenetic authentication of Ophiocordyceps sphecocephala from Lang Biang Biosphere Reserve, Lam Dong, Vietnam |
| title_sort | phylogenetic authentication of ophiocordyceps sphecocephala from lang biang biosphere reserve lam dong vietnam |
| topic | entomopathogenic fungi lang biang biosphere reserve ophiocordyceps phylogeny |
| url | https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/2641 |
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