Assessment of the Molecular Responses of an Ancient Angiosperm against Atypical Insect Oviposition: The Case of Hass Avocados and the Tephritid Fly <i>Anastrepha ludens</i>
<i>Anastrepha</i> spp. (Diptera: Tephritidae) infestations cause significant economic losses in commercial fruit production worldwide. However, some plants quickly counteract the insertion of eggs by females by generating neoplasia and hindering eclosion, as is the case for <i>Pers...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2060 |
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| author | Martín Aluja Mirna Vázquez-Rosas-Landa Daniel Cerqueda-García Juan L. Monribot-Villanueva Alma Altúzar-Molina Mónica Ramírez-Vázquez Olinda Velázquez-López Greta Rosas-Saito Alexandro G. Alonso-Sánchez Rafael Ortega-Casas Adrián José Enríquez-Valencia José A. Guerrero-Analco Enrique Ibarra-Laclette |
| author_facet | Martín Aluja Mirna Vázquez-Rosas-Landa Daniel Cerqueda-García Juan L. Monribot-Villanueva Alma Altúzar-Molina Mónica Ramírez-Vázquez Olinda Velázquez-López Greta Rosas-Saito Alexandro G. Alonso-Sánchez Rafael Ortega-Casas Adrián José Enríquez-Valencia José A. Guerrero-Analco Enrique Ibarra-Laclette |
| author_sort | Martín Aluja |
| collection | DOAJ |
| description | <i>Anastrepha</i> spp. (Diptera: Tephritidae) infestations cause significant economic losses in commercial fruit production worldwide. However, some plants quickly counteract the insertion of eggs by females by generating neoplasia and hindering eclosion, as is the case for <i>Persea americana</i> Mill., cv. Hass (Hass avocados). We followed a combined transcriptomics/metabolomics approach to identify the molecular mechanisms triggered by Hass avocados to detect and react to the oviposition of the pestiferous <i>Anastrepha ludens</i> (Loew). We evaluated two conditions: fruit damaged using a sterile pin (pin) and fruit oviposited by <i>A. ludens</i> females (ovi). We evaluated both of the conditions in a time course experiment covering five sampling points: without treatment (day 0), 20 min after the treatment (day 1), and days 3, 6, and 9 after the treatment. We identified 288 differentially expressed genes related to the treatments. Oviposition (and possibly bacteria on the eggs’ surface) induces a plant hypersensitive response (HR), triggering a chitin receptor, producing an oxidative burst, and synthesizing phytoalexins. We also observed a process of cell wall modification and polyphenols biosynthesis, which could lead to polymerization in the neoplastic tissue surrounding the eggs. |
| first_indexed | 2024-03-11T09:42:34Z |
| format | Article |
| id | doaj.art-1febf40217ae45afb15424f7aca12088 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:42:34Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1febf40217ae45afb15424f7aca120882023-11-16T16:51:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01243206010.3390/ijms24032060Assessment of the Molecular Responses of an Ancient Angiosperm against Atypical Insect Oviposition: The Case of Hass Avocados and the Tephritid Fly <i>Anastrepha ludens</i>Martín Aluja0Mirna Vázquez-Rosas-Landa1Daniel Cerqueda-García2Juan L. Monribot-Villanueva3Alma Altúzar-Molina4Mónica Ramírez-Vázquez5Olinda Velázquez-López6Greta Rosas-Saito7Alexandro G. Alonso-Sánchez8Rafael Ortega-Casas9Adrián José Enríquez-Valencia10José A. Guerrero-Analco11Enrique Ibarra-Laclette12Red de Manejo Biorracional de Plagas y Vectores, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Manejo Biorracional de Plagas y Vectores, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Manejo Biorracional de Plagas y Vectores, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Estudios Moleculares Avanzados, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Manejo Biorracional de Plagas y Vectores, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Estudios Moleculares Avanzados, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Estudios Moleculares Avanzados, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Estudios Moleculares Avanzados, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Estudios Moleculares Avanzados, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Manejo Biorracional de Plagas y Vectores, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Estudios Moleculares Avanzados, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Estudios Moleculares Avanzados, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, MexicoRed de Estudios Moleculares Avanzados, Instituto de Ecología, A.C.—INECOL, Clúster Científico y Tecnológico BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, Mexico<i>Anastrepha</i> spp. (Diptera: Tephritidae) infestations cause significant economic losses in commercial fruit production worldwide. However, some plants quickly counteract the insertion of eggs by females by generating neoplasia and hindering eclosion, as is the case for <i>Persea americana</i> Mill., cv. Hass (Hass avocados). We followed a combined transcriptomics/metabolomics approach to identify the molecular mechanisms triggered by Hass avocados to detect and react to the oviposition of the pestiferous <i>Anastrepha ludens</i> (Loew). We evaluated two conditions: fruit damaged using a sterile pin (pin) and fruit oviposited by <i>A. ludens</i> females (ovi). We evaluated both of the conditions in a time course experiment covering five sampling points: without treatment (day 0), 20 min after the treatment (day 1), and days 3, 6, and 9 after the treatment. We identified 288 differentially expressed genes related to the treatments. Oviposition (and possibly bacteria on the eggs’ surface) induces a plant hypersensitive response (HR), triggering a chitin receptor, producing an oxidative burst, and synthesizing phytoalexins. We also observed a process of cell wall modification and polyphenols biosynthesis, which could lead to polymerization in the neoplastic tissue surrounding the eggs.https://www.mdpi.com/1422-0067/24/3/2060plant–insect interactionsplant defense response to insect eggsneoplasiatranscriptomicsmetabolomics<i>Persea americana</i> cv. Hass |
| spellingShingle | Martín Aluja Mirna Vázquez-Rosas-Landa Daniel Cerqueda-García Juan L. Monribot-Villanueva Alma Altúzar-Molina Mónica Ramírez-Vázquez Olinda Velázquez-López Greta Rosas-Saito Alexandro G. Alonso-Sánchez Rafael Ortega-Casas Adrián José Enríquez-Valencia José A. Guerrero-Analco Enrique Ibarra-Laclette Assessment of the Molecular Responses of an Ancient Angiosperm against Atypical Insect Oviposition: The Case of Hass Avocados and the Tephritid Fly <i>Anastrepha ludens</i> International Journal of Molecular Sciences plant–insect interactions plant defense response to insect eggs neoplasia transcriptomics metabolomics <i>Persea americana</i> cv. Hass |
| title | Assessment of the Molecular Responses of an Ancient Angiosperm against Atypical Insect Oviposition: The Case of Hass Avocados and the Tephritid Fly <i>Anastrepha ludens</i> |
| title_full | Assessment of the Molecular Responses of an Ancient Angiosperm against Atypical Insect Oviposition: The Case of Hass Avocados and the Tephritid Fly <i>Anastrepha ludens</i> |
| title_fullStr | Assessment of the Molecular Responses of an Ancient Angiosperm against Atypical Insect Oviposition: The Case of Hass Avocados and the Tephritid Fly <i>Anastrepha ludens</i> |
| title_full_unstemmed | Assessment of the Molecular Responses of an Ancient Angiosperm against Atypical Insect Oviposition: The Case of Hass Avocados and the Tephritid Fly <i>Anastrepha ludens</i> |
| title_short | Assessment of the Molecular Responses of an Ancient Angiosperm against Atypical Insect Oviposition: The Case of Hass Avocados and the Tephritid Fly <i>Anastrepha ludens</i> |
| title_sort | assessment of the molecular responses of an ancient angiosperm against atypical insect oviposition the case of hass avocados and the tephritid fly i anastrepha ludens i |
| topic | plant–insect interactions plant defense response to insect eggs neoplasia transcriptomics metabolomics <i>Persea americana</i> cv. Hass |
| url | https://www.mdpi.com/1422-0067/24/3/2060 |
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