Plastid DNA sequences and oospore characters of some European taxa of Tolypella section Tolypella (Characeae) identify five clusters, including one new cryptic Tolypella taxon from Sardinia, but they do not coincide with current morphological descriptions
In Europe, the genus Tolypella (Characeae) comprises four to eight Tolypella taxa in sections Rothia and Tolypella that have been distinguished by vegetative morphology and gametangial characters such as antheridial size and oospore wall ornamentation. However, morphological differentiation is diffi...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1096181/full |
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| author | Anja Holzhausen Anja Holzhausen Petra Nowak Andreas Ballot Ralf Becker Jasmina Gebert Thomas Gregor Kenneth G. Karol Elisabeth Lambert William Pérez Uwe Raabe Susanne C. Schneider Nick Stewart Klaus van de Weyer Volker Wilde Hendrik Schubert |
| author_facet | Anja Holzhausen Anja Holzhausen Petra Nowak Andreas Ballot Ralf Becker Jasmina Gebert Thomas Gregor Kenneth G. Karol Elisabeth Lambert William Pérez Uwe Raabe Susanne C. Schneider Nick Stewart Klaus van de Weyer Volker Wilde Hendrik Schubert |
| author_sort | Anja Holzhausen |
| collection | DOAJ |
| description | In Europe, the genus Tolypella (Characeae) comprises four to eight Tolypella taxa in sections Rothia and Tolypella that have been distinguished by vegetative morphology and gametangial characters such as antheridial size and oospore wall ornamentation. However, morphological differentiation is difficult in some cases due to overlapping and variable vegetative features, which in many cases are difficult to observe clearly. To clarify the taxonomic status of the five European taxa of Tolypella in section Tolypella, sequence data of the plastid genes atpB, rbcL and psbC for Tolypella glomerata (Desv.) Leonh., Tolypella hispanica Allen, Tolypella nidifica (O.F. Müll.) A. Braun, Tolypella normaniana (Nordst.) Nordst. and Tolypella salina Cor. were combined with data on oospore morphology, including oospore wall ornamentation. Gene sequence data identified five distinct clusters, but they were not consistent with the morphologically identified five taxa. T. glomerata consisted of some of the samples morphologically identified as T. glomerata and seven samples of T. normaniana, while the remaining T. glomerata samples clustered with specimens of unclear affiliation (Tolypella sp.). We identified two clusters of T. hispanica within the European material: cluster T. hispanica I consisted of samples from various locations, whereas the second cluster (T. hispanica II) consisted of samples of T. hispanica from Sardinia Island. The remaining cluster consisted of all the specimens that had been determined as T. salina or T. nidifica in addition to two specimens of T. normaniana. Oospore morphology was most clearly distinguishable for T. glomerata. Oospore characteristics for all other taxa were not as informative but showed some geographical and/or environmentally influenced differences, especially for T. nidifica and T. salina. Our results suggest the need to further check the different taxonomy of Tolypella sect. Tolypella in which specimens normally identified as T. glomerata might be two different taxa, T. glomerata and an unidentified taxon; T. nidifica and T. salina are not separate taxa; T. normaniana is a diminutive variant of two different Tolypella taxa; and T. hispanica comprises two different taxa, one from the Mediterranean island Sardinia. |
| first_indexed | 2024-04-10T06:31:35Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-04-10T06:31:35Z |
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| spelling | doaj.art-1fec5552154540bf948a1106f8cf50292023-03-01T06:48:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.10961811096181Plastid DNA sequences and oospore characters of some European taxa of Tolypella section Tolypella (Characeae) identify five clusters, including one new cryptic Tolypella taxon from Sardinia, but they do not coincide with current morphological descriptionsAnja Holzhausen0Anja Holzhausen1Petra Nowak2Andreas Ballot3Ralf Becker4Jasmina Gebert5Thomas Gregor6Kenneth G. Karol7Elisabeth Lambert8William Pérez9Uwe Raabe10Susanne C. Schneider11Nick Stewart12Klaus van de Weyer13Volker Wilde14Hendrik Schubert15Institute of Biological Sciences, Aquatic Ecology, University Rostock, Rostock, GermanyDepartment of Biology, Plant Cell Biology, University Marburg, Marburg, GermanyInstitute of Biological Sciences, Aquatic Ecology, University Rostock, Rostock, GermanyNorwegian Institute for Water Research, Oslo, NorwayIndependent Researcher, Oldenburg, GermanyInstitute of Biological Sciences, Aquatic Ecology, University Rostock, Rostock, GermanySenckenberg Forschungsinstitut und Naturmuseum, Botanik und Molekulare Evolutionsforschung, Frankfurt am Main, GermanyProgram for Molecular Systematics Studies, The New York Botanical Garden, Bronx, NY, United StatesDépartement de Biologie Environnement, Faculté des Sciences, Université Catholique de l’Ouest, Angers, FranceProgram for Molecular Systematics Studies, The New York Botanical Garden, Bronx, NY, United StatesIndependent Researcher, Marl, GermanyNorwegian Institute for Water Research, Oslo, NorwayIndependent Researcher, Glastonbury, United Kingdom0lanaplan GbR, Nettetal, Germany1Paläontologie und Historische Geologie, Paläobotanik, Senkenberg Forschungsinstitut und Naturmuseum, Frankfurt am Main, GermanyInstitute of Biological Sciences, Aquatic Ecology, University Rostock, Rostock, GermanyIn Europe, the genus Tolypella (Characeae) comprises four to eight Tolypella taxa in sections Rothia and Tolypella that have been distinguished by vegetative morphology and gametangial characters such as antheridial size and oospore wall ornamentation. However, morphological differentiation is difficult in some cases due to overlapping and variable vegetative features, which in many cases are difficult to observe clearly. To clarify the taxonomic status of the five European taxa of Tolypella in section Tolypella, sequence data of the plastid genes atpB, rbcL and psbC for Tolypella glomerata (Desv.) Leonh., Tolypella hispanica Allen, Tolypella nidifica (O.F. Müll.) A. Braun, Tolypella normaniana (Nordst.) Nordst. and Tolypella salina Cor. were combined with data on oospore morphology, including oospore wall ornamentation. Gene sequence data identified five distinct clusters, but they were not consistent with the morphologically identified five taxa. T. glomerata consisted of some of the samples morphologically identified as T. glomerata and seven samples of T. normaniana, while the remaining T. glomerata samples clustered with specimens of unclear affiliation (Tolypella sp.). We identified two clusters of T. hispanica within the European material: cluster T. hispanica I consisted of samples from various locations, whereas the second cluster (T. hispanica II) consisted of samples of T. hispanica from Sardinia Island. The remaining cluster consisted of all the specimens that had been determined as T. salina or T. nidifica in addition to two specimens of T. normaniana. Oospore morphology was most clearly distinguishable for T. glomerata. Oospore characteristics for all other taxa were not as informative but showed some geographical and/or environmentally influenced differences, especially for T. nidifica and T. salina. Our results suggest the need to further check the different taxonomy of Tolypella sect. Tolypella in which specimens normally identified as T. glomerata might be two different taxa, T. glomerata and an unidentified taxon; T. nidifica and T. salina are not separate taxa; T. normaniana is a diminutive variant of two different Tolypella taxa; and T. hispanica comprises two different taxa, one from the Mediterranean island Sardinia.https://www.frontiersin.org/articles/10.3389/fpls.2023.1096181/fullcharophytesgenetic diversityoospore morphologyphylogenyTolypellataxonomic concepts |
| spellingShingle | Anja Holzhausen Anja Holzhausen Petra Nowak Andreas Ballot Ralf Becker Jasmina Gebert Thomas Gregor Kenneth G. Karol Elisabeth Lambert William Pérez Uwe Raabe Susanne C. Schneider Nick Stewart Klaus van de Weyer Volker Wilde Hendrik Schubert Plastid DNA sequences and oospore characters of some European taxa of Tolypella section Tolypella (Characeae) identify five clusters, including one new cryptic Tolypella taxon from Sardinia, but they do not coincide with current morphological descriptions Frontiers in Plant Science charophytes genetic diversity oospore morphology phylogeny Tolypella taxonomic concepts |
| title | Plastid DNA sequences and oospore characters of some European taxa of Tolypella section Tolypella (Characeae) identify five clusters, including one new cryptic Tolypella taxon from Sardinia, but they do not coincide with current morphological descriptions |
| title_full | Plastid DNA sequences and oospore characters of some European taxa of Tolypella section Tolypella (Characeae) identify five clusters, including one new cryptic Tolypella taxon from Sardinia, but they do not coincide with current morphological descriptions |
| title_fullStr | Plastid DNA sequences and oospore characters of some European taxa of Tolypella section Tolypella (Characeae) identify five clusters, including one new cryptic Tolypella taxon from Sardinia, but they do not coincide with current morphological descriptions |
| title_full_unstemmed | Plastid DNA sequences and oospore characters of some European taxa of Tolypella section Tolypella (Characeae) identify five clusters, including one new cryptic Tolypella taxon from Sardinia, but they do not coincide with current morphological descriptions |
| title_short | Plastid DNA sequences and oospore characters of some European taxa of Tolypella section Tolypella (Characeae) identify five clusters, including one new cryptic Tolypella taxon from Sardinia, but they do not coincide with current morphological descriptions |
| title_sort | plastid dna sequences and oospore characters of some european taxa of tolypella section tolypella characeae identify five clusters including one new cryptic tolypella taxon from sardinia but they do not coincide with current morphological descriptions |
| topic | charophytes genetic diversity oospore morphology phylogeny Tolypella taxonomic concepts |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1096181/full |
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