Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair
<p>Abstract</p> <p>Background</p> <p>The highly homologous PE_PGRS (Proline-glutamic acid_polymorphic GC-rich repetitive sequence) genes are members of the PE multigene family which is found only in mycobacteria. PE genes are particularly abundant within the genomes of...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2006-12-01
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| Series: | BMC Evolutionary Biology |
| Online Access: | http://www.biomedcentral.com/1471-2148/6/107 |
| _version_ | 1811191116468322304 |
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| author | Kurepina Natalia Huard Richard C Cataldi Angel Fabre Michel Suffys Philip Rastogi Nalin Sola Christophe Vincent Véronique Namouchi Amine Gey van Pittius Nicolaas C Karboul Anis Kreiswirth Barry Ho John L Gutierrez M Cristina Mardassi Helmi |
| author_facet | Kurepina Natalia Huard Richard C Cataldi Angel Fabre Michel Suffys Philip Rastogi Nalin Sola Christophe Vincent Véronique Namouchi Amine Gey van Pittius Nicolaas C Karboul Anis Kreiswirth Barry Ho John L Gutierrez M Cristina Mardassi Helmi |
| author_sort | Kurepina Natalia |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The highly homologous PE_PGRS (Proline-glutamic acid_polymorphic GC-rich repetitive sequence) genes are members of the PE multigene family which is found only in mycobacteria. PE genes are particularly abundant within the genomes of pathogenic mycobacteria where they seem to have expanded as a result of gene duplication events. PE_PGRS genes are characterized by their high GC content and extensive repetitive sequences, making them prone to recombination events and genetic variability.</p> <p>Results</p> <p>Comparative sequence analysis of <it>Mycobacterium tuberculosis </it>genes PE_PGRS17 (Rv0978c) and PE_PGRS18 (Rv0980c) revealed a striking genetic variation associated with this typical tandem duplicate. In comparison to the <it>M. tuberculosis </it>reference strain H37Rv, the variation (named the 12/40 polymorphism) consists of an in-frame 12-bp insertion invariably accompanied by a set of 40 single nucleotide polymorphisms (SNPs) that occurs either in PE_PGRS17 or in both genes. Sequence analysis of the paralogous genes in a representative set of worldwide distributed tubercle bacilli isolates revealed data which supported previously proposed evolutionary scenarios for the <it>M. tuberculosis </it>complex (MTBC) and confirmed the very ancient origin of "<it>M. canettii</it>" and other smooth tubercle bacilli. Strikingly, the identified polymorphism appears to be coincident with the emergence of the post-bottleneck successful clone from which the MTBC expanded. Furthermore, the findings provide direct and clear evidence for the natural occurrence of gene conversion in mycobacteria, which appears to be restricted to modern <it>M. tuberculosis </it>strains.</p> <p>Conclusion</p> <p>This study provides a new perspective to explore the molecular events that accompanied the evolution, clonal expansion, and recent diversification of tubercle bacilli.</p> |
| first_indexed | 2024-04-11T15:01:22Z |
| format | Article |
| id | doaj.art-1ff1110e80454a47a9f30c1941a22f2e |
| institution | Directory Open Access Journal |
| issn | 1471-2148 |
| language | English |
| last_indexed | 2024-04-11T15:01:22Z |
| publishDate | 2006-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Evolutionary Biology |
| spelling | doaj.art-1ff1110e80454a47a9f30c1941a22f2e2022-12-22T04:16:57ZengBMCBMC Evolutionary Biology1471-21482006-12-016110710.1186/1471-2148-6-107Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pairKurepina NataliaHuard Richard CCataldi AngelFabre MichelSuffys PhilipRastogi NalinSola ChristopheVincent VéroniqueNamouchi AmineGey van Pittius Nicolaas CKarboul AnisKreiswirth BarryHo John LGutierrez M CristinaMardassi Helmi<p>Abstract</p> <p>Background</p> <p>The highly homologous PE_PGRS (Proline-glutamic acid_polymorphic GC-rich repetitive sequence) genes are members of the PE multigene family which is found only in mycobacteria. PE genes are particularly abundant within the genomes of pathogenic mycobacteria where they seem to have expanded as a result of gene duplication events. PE_PGRS genes are characterized by their high GC content and extensive repetitive sequences, making them prone to recombination events and genetic variability.</p> <p>Results</p> <p>Comparative sequence analysis of <it>Mycobacterium tuberculosis </it>genes PE_PGRS17 (Rv0978c) and PE_PGRS18 (Rv0980c) revealed a striking genetic variation associated with this typical tandem duplicate. In comparison to the <it>M. tuberculosis </it>reference strain H37Rv, the variation (named the 12/40 polymorphism) consists of an in-frame 12-bp insertion invariably accompanied by a set of 40 single nucleotide polymorphisms (SNPs) that occurs either in PE_PGRS17 or in both genes. Sequence analysis of the paralogous genes in a representative set of worldwide distributed tubercle bacilli isolates revealed data which supported previously proposed evolutionary scenarios for the <it>M. tuberculosis </it>complex (MTBC) and confirmed the very ancient origin of "<it>M. canettii</it>" and other smooth tubercle bacilli. Strikingly, the identified polymorphism appears to be coincident with the emergence of the post-bottleneck successful clone from which the MTBC expanded. Furthermore, the findings provide direct and clear evidence for the natural occurrence of gene conversion in mycobacteria, which appears to be restricted to modern <it>M. tuberculosis </it>strains.</p> <p>Conclusion</p> <p>This study provides a new perspective to explore the molecular events that accompanied the evolution, clonal expansion, and recent diversification of tubercle bacilli.</p>http://www.biomedcentral.com/1471-2148/6/107 |
| spellingShingle | Kurepina Natalia Huard Richard C Cataldi Angel Fabre Michel Suffys Philip Rastogi Nalin Sola Christophe Vincent Véronique Namouchi Amine Gey van Pittius Nicolaas C Karboul Anis Kreiswirth Barry Ho John L Gutierrez M Cristina Mardassi Helmi Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair BMC Evolutionary Biology |
| title | Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair |
| title_full | Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair |
| title_fullStr | Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair |
| title_full_unstemmed | Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair |
| title_short | Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair |
| title_sort | insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a pe pgrs duplicated gene pair |
| url | http://www.biomedcentral.com/1471-2148/6/107 |
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