Subzero Nonfreezing Hypothermia with Insect Antifreeze Protein Dramatically Improves Survival Rate of Mammalian Cells
Cells for therapeutic use are often preserved at +4 °C, and the storage period is generally limited to 2–3 days. Here, we report that the survival rate (%) of mammalian cells is improved to 10–20 days when they are preserved with a subzero supercooled solution containing the antifreeze protein (AFP)...
Main Authors: | Akari Yamauchi, Ai Miura, Hidemasa Kondo, Tatsuya Arai, Yuji C. Sasaki, Sakae Tsuda |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-11-01
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Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/22/23/12680 |
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