Characterization of the Pyrroloquinoline Quinone Producing <i>Rhodopseudomonas palustris</i> as a Plant Growth-Promoting Bacterium under Photoautotrophic and Photoheterotrophic Culture Conditions
<i>Rhodopseudomonas palustris</i> is a purple non-sulfide bacterium (PNSB), and some strains have been proven to promote plant growth. However, the mechanism underlying the effect of these PNSBs remains limited. Based on genetic information, <i>R. palustris</i> possesses the...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| author | Shou-Chen Lo Shang-Yieng Tsai Wei-Hsiang Chang I-Chen Wu Nga-Lai Sou Shih-Hsun Walter Hung En-Pei Isabel Chiang Chieh-Chen Huang |
| author_facet | Shou-Chen Lo Shang-Yieng Tsai Wei-Hsiang Chang I-Chen Wu Nga-Lai Sou Shih-Hsun Walter Hung En-Pei Isabel Chiang Chieh-Chen Huang |
| author_sort | Shou-Chen Lo |
| collection | DOAJ |
| description | <i>Rhodopseudomonas palustris</i> is a purple non-sulfide bacterium (PNSB), and some strains have been proven to promote plant growth. However, the mechanism underlying the effect of these PNSBs remains limited. Based on genetic information, <i>R. palustris</i> possesses the ability to produce pyrroloquinoline quinone (PQQ). PQQ is known to play a crucial role in stimulating plant growth, facilitating phosphorous solubilization, and acting as a reactive oxygen species scavenger. However, it is still uncertain whether growth conditions influence <i>R. palustris</i>’s production of PQQ and other characteristics. In the present study, it was found that <i>R. palustris</i> exhibited a higher expression of genes related to PQQ synthesis under autotrophic culture conditions as compared to acetate culture conditions. Moreover, similar patterns were observed for phosphorous solubilization and siderophore activity, both of which are recognized to contribute to plant-growth benefits. However, these PNSB culture conditions did not show differences in <i>Arabidopsis</i> growth experiments, indicating that there may be other factors influencing plant growth in addition to PQQ content. Furthermore, the endophytic bacterial strains isolated from <i>Arabidopsis</i> exhibited differences according to the PNSB culture conditions. These findings imply that, depending on the PNSB’s growing conditions, it may interact with various soil bacteria and facilitate their infiltration into plants. |
| first_indexed | 2024-03-10T22:40:00Z |
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| id | doaj.art-1ff6169fbeb14eb3a49631af79aebdfd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:40:00Z |
| publishDate | 2023-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1ff6169fbeb14eb3a49631af79aebdfd2023-11-19T11:07:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181408010.3390/ijms241814080Characterization of the Pyrroloquinoline Quinone Producing <i>Rhodopseudomonas palustris</i> as a Plant Growth-Promoting Bacterium under Photoautotrophic and Photoheterotrophic Culture ConditionsShou-Chen Lo0Shang-Yieng Tsai1Wei-Hsiang Chang2I-Chen Wu3Nga-Lai Sou4Shih-Hsun Walter Hung5En-Pei Isabel Chiang6Chieh-Chen Huang7Department of Life Sciences, National Chung Hsing University, Taichung 402202, TaiwanDepartment of Life Sciences, National Chung Hsing University, Taichung 402202, TaiwanDepartment of Life Sciences, National Chung Hsing University, Taichung 402202, TaiwanDepartment of Life Sciences, National Chung Hsing University, Taichung 402202, TaiwanDepartment of Food Science and Biotechnology, National Chung Hsing University, Taichung 402202, TaiwanDepartment of Life Sciences, National Chung Hsing University, Taichung 402202, TaiwanDepartment of Food Science and Biotechnology, National Chung Hsing University, Taichung 402202, TaiwanDepartment of Life Sciences, National Chung Hsing University, Taichung 402202, Taiwan<i>Rhodopseudomonas palustris</i> is a purple non-sulfide bacterium (PNSB), and some strains have been proven to promote plant growth. However, the mechanism underlying the effect of these PNSBs remains limited. Based on genetic information, <i>R. palustris</i> possesses the ability to produce pyrroloquinoline quinone (PQQ). PQQ is known to play a crucial role in stimulating plant growth, facilitating phosphorous solubilization, and acting as a reactive oxygen species scavenger. However, it is still uncertain whether growth conditions influence <i>R. palustris</i>’s production of PQQ and other characteristics. In the present study, it was found that <i>R. palustris</i> exhibited a higher expression of genes related to PQQ synthesis under autotrophic culture conditions as compared to acetate culture conditions. Moreover, similar patterns were observed for phosphorous solubilization and siderophore activity, both of which are recognized to contribute to plant-growth benefits. However, these PNSB culture conditions did not show differences in <i>Arabidopsis</i> growth experiments, indicating that there may be other factors influencing plant growth in addition to PQQ content. Furthermore, the endophytic bacterial strains isolated from <i>Arabidopsis</i> exhibited differences according to the PNSB culture conditions. These findings imply that, depending on the PNSB’s growing conditions, it may interact with various soil bacteria and facilitate their infiltration into plants.https://www.mdpi.com/1422-0067/24/18/14080<i>Rhodopseudomonas palustris</i>purple non-sulfide bacteriumpyrroloquinoline quinone<i>Arabidopsis</i>autotrophic cultureendophyte |
| spellingShingle | Shou-Chen Lo Shang-Yieng Tsai Wei-Hsiang Chang I-Chen Wu Nga-Lai Sou Shih-Hsun Walter Hung En-Pei Isabel Chiang Chieh-Chen Huang Characterization of the Pyrroloquinoline Quinone Producing <i>Rhodopseudomonas palustris</i> as a Plant Growth-Promoting Bacterium under Photoautotrophic and Photoheterotrophic Culture Conditions International Journal of Molecular Sciences <i>Rhodopseudomonas palustris</i> purple non-sulfide bacterium pyrroloquinoline quinone <i>Arabidopsis</i> autotrophic culture endophyte |
| title | Characterization of the Pyrroloquinoline Quinone Producing <i>Rhodopseudomonas palustris</i> as a Plant Growth-Promoting Bacterium under Photoautotrophic and Photoheterotrophic Culture Conditions |
| title_full | Characterization of the Pyrroloquinoline Quinone Producing <i>Rhodopseudomonas palustris</i> as a Plant Growth-Promoting Bacterium under Photoautotrophic and Photoheterotrophic Culture Conditions |
| title_fullStr | Characterization of the Pyrroloquinoline Quinone Producing <i>Rhodopseudomonas palustris</i> as a Plant Growth-Promoting Bacterium under Photoautotrophic and Photoheterotrophic Culture Conditions |
| title_full_unstemmed | Characterization of the Pyrroloquinoline Quinone Producing <i>Rhodopseudomonas palustris</i> as a Plant Growth-Promoting Bacterium under Photoautotrophic and Photoheterotrophic Culture Conditions |
| title_short | Characterization of the Pyrroloquinoline Quinone Producing <i>Rhodopseudomonas palustris</i> as a Plant Growth-Promoting Bacterium under Photoautotrophic and Photoheterotrophic Culture Conditions |
| title_sort | characterization of the pyrroloquinoline quinone producing i rhodopseudomonas palustris i as a plant growth promoting bacterium under photoautotrophic and photoheterotrophic culture conditions |
| topic | <i>Rhodopseudomonas palustris</i> purple non-sulfide bacterium pyrroloquinoline quinone <i>Arabidopsis</i> autotrophic culture endophyte |
| url | https://www.mdpi.com/1422-0067/24/18/14080 |
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