Bombyx mori Silk Fibroin Regeneration in Solution of Lanthanide Ions: A Systematic Investigation
Silk Fibroin (SF) obtained from Bombyx mori is a very attractive biopolymer that can be useful for many technological applications, from optoelectronics and photonics to biomedicine. It can be processed from aqueous solutions to obtain many scaffolds. SF dissolution is possible only with the mediati...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-06-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.653033/full |
| _version_ | 1828961839719907328 |
|---|---|
| author | Giorgio Rizzo Marco Lo Presti Cinzia Giannini Teresa Sibillano Antonella Milella Giulia Guidetti Roberta Musio Fiorenzo G. Omenetto Gianluca M. Farinola Gianluca M. Farinola |
| author_facet | Giorgio Rizzo Marco Lo Presti Cinzia Giannini Teresa Sibillano Antonella Milella Giulia Guidetti Roberta Musio Fiorenzo G. Omenetto Gianluca M. Farinola Gianluca M. Farinola |
| author_sort | Giorgio Rizzo |
| collection | DOAJ |
| description | Silk Fibroin (SF) obtained from Bombyx mori is a very attractive biopolymer that can be useful for many technological applications, from optoelectronics and photonics to biomedicine. It can be processed from aqueous solutions to obtain many scaffolds. SF dissolution is possible only with the mediation of chaotropic salts that disrupt the secondary structure of the protein. As a consequence, recovered materials have disordered structures. In a previous paper, it was shown that, by modifying the standard Ajisawa’s method by using a lanthanide salt, CeCl3, as the chaotropic agent, it is possible to regenerate SF as a fibrous material with a very ordered structure, similar to that of the pristine fiber, and doped with Ce+3 ions. Since SF exhibits a moderate fluorescence which can be enhanced by the incorporation of organic molecules, ions and nanoparticles, the possibility of doping it with lanthanide ions could be an appealing approach for the development of new photonic systems. Here, a systematic investigation of the behavior of degummed SF in the presence of all lanthanide ions, Ln+3, is reported. It has been found that all lanthanide chlorides are chaotropic salts for solubilizing SF. Ln+3 ions at the beginning and the end of the series (La+3, Pr+3, Er+3, Tm+3, Yb+3, Lu+3) favor the reprecipitation of fibrous SF as already found for Ce+3. In most cases, the obtained fiber preserves the morphological and structural features of the pristine SF. With the exception of SF treated with La+3, Tm+3, and Lu+3, for all the fibers re-precipitated a concentration of Ln+3 between 0.2 and 0.4% at was measured, comparable to that measured for Ce+3-doped SF. |
| first_indexed | 2024-12-14T09:53:01Z |
| format | Article |
| id | doaj.art-1ff78fd887f94e06b8ecb562803fa12f |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-14T09:53:01Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-1ff78fd887f94e06b8ecb562803fa12f2022-12-21T23:07:28ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-06-01910.3389/fbioe.2021.653033653033Bombyx mori Silk Fibroin Regeneration in Solution of Lanthanide Ions: A Systematic InvestigationGiorgio Rizzo0Marco Lo Presti1Cinzia Giannini2Teresa Sibillano3Antonella Milella4Giulia Guidetti5Roberta Musio6Fiorenzo G. Omenetto7Gianluca M. Farinola8Gianluca M. Farinola9Dipartimento di Chimica, Università degli Studi di Bari “Aldo Moro”, Bari, ItalySilklab, Department of Biomedical Engineering, Tufts University, Medford, MA, United StatesCNR IC–Institute of Crystallography, Bari, ItalyCNR IC–Institute of Crystallography, Bari, ItalyDipartimento di Chimica, Università degli Studi di Bari “Aldo Moro”, Bari, ItalySilklab, Department of Biomedical Engineering, Tufts University, Medford, MA, United StatesDipartimento di Chimica, Università degli Studi di Bari “Aldo Moro”, Bari, ItalySilklab, Department of Biomedical Engineering, Tufts University, Medford, MA, United StatesDipartimento di Chimica, Università degli Studi di Bari “Aldo Moro”, Bari, ItalySilklab, Department of Biomedical Engineering, Tufts University, Medford, MA, United StatesSilk Fibroin (SF) obtained from Bombyx mori is a very attractive biopolymer that can be useful for many technological applications, from optoelectronics and photonics to biomedicine. It can be processed from aqueous solutions to obtain many scaffolds. SF dissolution is possible only with the mediation of chaotropic salts that disrupt the secondary structure of the protein. As a consequence, recovered materials have disordered structures. In a previous paper, it was shown that, by modifying the standard Ajisawa’s method by using a lanthanide salt, CeCl3, as the chaotropic agent, it is possible to regenerate SF as a fibrous material with a very ordered structure, similar to that of the pristine fiber, and doped with Ce+3 ions. Since SF exhibits a moderate fluorescence which can be enhanced by the incorporation of organic molecules, ions and nanoparticles, the possibility of doping it with lanthanide ions could be an appealing approach for the development of new photonic systems. Here, a systematic investigation of the behavior of degummed SF in the presence of all lanthanide ions, Ln+3, is reported. It has been found that all lanthanide chlorides are chaotropic salts for solubilizing SF. Ln+3 ions at the beginning and the end of the series (La+3, Pr+3, Er+3, Tm+3, Yb+3, Lu+3) favor the reprecipitation of fibrous SF as already found for Ce+3. In most cases, the obtained fiber preserves the morphological and structural features of the pristine SF. With the exception of SF treated with La+3, Tm+3, and Lu+3, for all the fibers re-precipitated a concentration of Ln+3 between 0.2 and 0.4% at was measured, comparable to that measured for Ce+3-doped SF.https://www.frontiersin.org/articles/10.3389/fbioe.2021.653033/fullsilk fibroinlanthanide doped fibroinsilk fibroin dissolutionsilk fibroin recoverybiomaterials |
| spellingShingle | Giorgio Rizzo Marco Lo Presti Cinzia Giannini Teresa Sibillano Antonella Milella Giulia Guidetti Roberta Musio Fiorenzo G. Omenetto Gianluca M. Farinola Gianluca M. Farinola Bombyx mori Silk Fibroin Regeneration in Solution of Lanthanide Ions: A Systematic Investigation Frontiers in Bioengineering and Biotechnology silk fibroin lanthanide doped fibroin silk fibroin dissolution silk fibroin recovery biomaterials |
| title | Bombyx mori Silk Fibroin Regeneration in Solution of Lanthanide Ions: A Systematic Investigation |
| title_full | Bombyx mori Silk Fibroin Regeneration in Solution of Lanthanide Ions: A Systematic Investigation |
| title_fullStr | Bombyx mori Silk Fibroin Regeneration in Solution of Lanthanide Ions: A Systematic Investigation |
| title_full_unstemmed | Bombyx mori Silk Fibroin Regeneration in Solution of Lanthanide Ions: A Systematic Investigation |
| title_short | Bombyx mori Silk Fibroin Regeneration in Solution of Lanthanide Ions: A Systematic Investigation |
| title_sort | bombyx mori silk fibroin regeneration in solution of lanthanide ions a systematic investigation |
| topic | silk fibroin lanthanide doped fibroin silk fibroin dissolution silk fibroin recovery biomaterials |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.653033/full |
| work_keys_str_mv | AT giorgiorizzo bombyxmorisilkfibroinregenerationinsolutionoflanthanideionsasystematicinvestigation AT marcolopresti bombyxmorisilkfibroinregenerationinsolutionoflanthanideionsasystematicinvestigation AT cinziagiannini bombyxmorisilkfibroinregenerationinsolutionoflanthanideionsasystematicinvestigation AT teresasibillano bombyxmorisilkfibroinregenerationinsolutionoflanthanideionsasystematicinvestigation AT antonellamilella bombyxmorisilkfibroinregenerationinsolutionoflanthanideionsasystematicinvestigation AT giuliaguidetti bombyxmorisilkfibroinregenerationinsolutionoflanthanideionsasystematicinvestigation AT robertamusio bombyxmorisilkfibroinregenerationinsolutionoflanthanideionsasystematicinvestigation AT fiorenzogomenetto bombyxmorisilkfibroinregenerationinsolutionoflanthanideionsasystematicinvestigation AT gianlucamfarinola bombyxmorisilkfibroinregenerationinsolutionoflanthanideionsasystematicinvestigation AT gianlucamfarinola bombyxmorisilkfibroinregenerationinsolutionoflanthanideionsasystematicinvestigation |