Molecular characterization of <it>Histoplasma capsulatum </it>isolated from an outbreak in treasure hunters <it>Histoplasma capsulatum </it>in treasure hunters
<p>Abstract</p> <p>Background</p> <p>In Mexico, primary pulmonary histoplasmosis is the most relevant clinical form of the disease. The geographical distribution of specific strains of <it>Histoplasma capsulatum </it>circulating in Mexico has not been fully...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-09-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/10/264 |
| _version_ | 1830281850186104832 |
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| author | Muñoz Bertha Martínez María Á Palma Gabriel Ramírez Amado Frías María G Reyes María R Taylor María L Higuera Anjarath L Corcho Alexander Manjarrez María E |
| author_facet | Muñoz Bertha Martínez María Á Palma Gabriel Ramírez Amado Frías María G Reyes María R Taylor María L Higuera Anjarath L Corcho Alexander Manjarrez María E |
| author_sort | Muñoz Bertha |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In Mexico, primary pulmonary histoplasmosis is the most relevant clinical form of the disease. The geographical distribution of specific strains of <it>Histoplasma capsulatum </it>circulating in Mexico has not been fully established. Outbreaks must be reported in order to have current, updated information on this disease, identifying new endemic areas, manner of exposure to the fungi, and molecular characterization of the causative agents. We report a recent outbreak of histoplasmosis in treasure hunters and the molecular characterization of two isolates obtained from these patients.</p> <p>Methods</p> <p>Six patients admitted to the National Institute of Respiratory Diseases (INER) in Mexico City presented severe respiratory symptoms suggestive of histoplasmosis. They acquired the infection in the Veracruz (VZ) endemic zone. Diagnosis was made by X-ray and Computed tomography (CT), liver function, immunological techniques, and culture. Identification of <it>H. capsulatum </it>isolates was confirmed by using Polymerase chain reaction (PCR) was conducted with a probe from the M antigen, and the isolates were characterized by means of Random amplification of polymorphic DNA (RAPD)-PCR employed the 1253 oligonucleotide and a mixture of oligonucleotides 1281 and 1283. These were compared to eight reference strain isolates from neighboring areas.</p> <p>Results</p> <p>X-ray and CT revealed disseminated micronodular images throughout lung parenchyma, as well as bilateral retrocaval, prevascular, subcarinal, and hilar adenopathies, hepatosplenomegaly, and altered liver function tests. Five of the six patients developed disseminated histoplasmosis. Two <it>H. capsulatum </it>strains were isolated. The same band profile was detected in both strains, indicating that both isolates corresponded to the sole <it>H. capsulatum </it>strain. Molecular characterization of the isolates was similar in 100% with the EH-53 Hidalgo human (HG) strain (reference strain integrated into the LAm A clade described for Latin America).</p> <p>Conclusions</p> <p>The two isolates appeared to possess the same polymorphic pattern; they are indistinguishable from each other and from EH-53. It is important to remain updated on recent outbreaks of histoplasmosis, the manner of exposure to the fungi, as well as the molecular characterization of the isolates. The severity of cases indicates that this strain is highly virulent and that it is probably prevalent in Hidalgo and Veracruz states.</p> |
| first_indexed | 2024-12-19T02:25:45Z |
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| id | doaj.art-1ffb2b94af2c4bdfb9f9f1a06198f460 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-19T02:25:45Z |
| publishDate | 2010-09-01 |
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| series | BMC Infectious Diseases |
| spelling | doaj.art-1ffb2b94af2c4bdfb9f9f1a06198f4602022-12-21T20:39:54ZengBMCBMC Infectious Diseases1471-23342010-09-0110126410.1186/1471-2334-10-264Molecular characterization of <it>Histoplasma capsulatum </it>isolated from an outbreak in treasure hunters <it>Histoplasma capsulatum </it>in treasure huntersMuñoz BerthaMartínez María ÁPalma GabrielRamírez AmadoFrías María GReyes María RTaylor María LHiguera Anjarath LCorcho AlexanderManjarrez María E<p>Abstract</p> <p>Background</p> <p>In Mexico, primary pulmonary histoplasmosis is the most relevant clinical form of the disease. The geographical distribution of specific strains of <it>Histoplasma capsulatum </it>circulating in Mexico has not been fully established. Outbreaks must be reported in order to have current, updated information on this disease, identifying new endemic areas, manner of exposure to the fungi, and molecular characterization of the causative agents. We report a recent outbreak of histoplasmosis in treasure hunters and the molecular characterization of two isolates obtained from these patients.</p> <p>Methods</p> <p>Six patients admitted to the National Institute of Respiratory Diseases (INER) in Mexico City presented severe respiratory symptoms suggestive of histoplasmosis. They acquired the infection in the Veracruz (VZ) endemic zone. Diagnosis was made by X-ray and Computed tomography (CT), liver function, immunological techniques, and culture. Identification of <it>H. capsulatum </it>isolates was confirmed by using Polymerase chain reaction (PCR) was conducted with a probe from the M antigen, and the isolates were characterized by means of Random amplification of polymorphic DNA (RAPD)-PCR employed the 1253 oligonucleotide and a mixture of oligonucleotides 1281 and 1283. These were compared to eight reference strain isolates from neighboring areas.</p> <p>Results</p> <p>X-ray and CT revealed disseminated micronodular images throughout lung parenchyma, as well as bilateral retrocaval, prevascular, subcarinal, and hilar adenopathies, hepatosplenomegaly, and altered liver function tests. Five of the six patients developed disseminated histoplasmosis. Two <it>H. capsulatum </it>strains were isolated. The same band profile was detected in both strains, indicating that both isolates corresponded to the sole <it>H. capsulatum </it>strain. Molecular characterization of the isolates was similar in 100% with the EH-53 Hidalgo human (HG) strain (reference strain integrated into the LAm A clade described for Latin America).</p> <p>Conclusions</p> <p>The two isolates appeared to possess the same polymorphic pattern; they are indistinguishable from each other and from EH-53. It is important to remain updated on recent outbreaks of histoplasmosis, the manner of exposure to the fungi, as well as the molecular characterization of the isolates. The severity of cases indicates that this strain is highly virulent and that it is probably prevalent in Hidalgo and Veracruz states.</p>http://www.biomedcentral.com/1471-2334/10/264 |
| spellingShingle | Muñoz Bertha Martínez María Á Palma Gabriel Ramírez Amado Frías María G Reyes María R Taylor María L Higuera Anjarath L Corcho Alexander Manjarrez María E Molecular characterization of <it>Histoplasma capsulatum </it>isolated from an outbreak in treasure hunters <it>Histoplasma capsulatum </it>in treasure hunters BMC Infectious Diseases |
| title | Molecular characterization of <it>Histoplasma capsulatum </it>isolated from an outbreak in treasure hunters <it>Histoplasma capsulatum </it>in treasure hunters |
| title_full | Molecular characterization of <it>Histoplasma capsulatum </it>isolated from an outbreak in treasure hunters <it>Histoplasma capsulatum </it>in treasure hunters |
| title_fullStr | Molecular characterization of <it>Histoplasma capsulatum </it>isolated from an outbreak in treasure hunters <it>Histoplasma capsulatum </it>in treasure hunters |
| title_full_unstemmed | Molecular characterization of <it>Histoplasma capsulatum </it>isolated from an outbreak in treasure hunters <it>Histoplasma capsulatum </it>in treasure hunters |
| title_short | Molecular characterization of <it>Histoplasma capsulatum </it>isolated from an outbreak in treasure hunters <it>Histoplasma capsulatum </it>in treasure hunters |
| title_sort | molecular characterization of it histoplasma capsulatum it isolated from an outbreak in treasure hunters it histoplasma capsulatum it in treasure hunters |
| url | http://www.biomedcentral.com/1471-2334/10/264 |
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