Detection of Yersinia ruckeri in naturally infected rainbow trout (Oncorhynchus mykiss Walbaum, 1792) from trout farms in Anatolia, Turkey
The present study aimed to detect Yersinia ruckeri bacteria in fish samples collected from the rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) farms in the South Eastern Anatolia Region of Turkey through bacteriological culturing and polymerase chain reaction. Separate visits were made to trout...
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Universidad del Zulia
2023-07-01
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| Series: | Revista Científica |
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| Online Access: | http://www.produccioncientificaluz.org/index.php/cientifica/article/view/40607 |
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| author | Filiz Özcan Neval Berrin Arserim Muzaffer Aydın Ketani Kasım Aydin Nurdan Karacan–Sever Nida Özcan Burçak Aslan–Çelik |
| author_facet | Filiz Özcan Neval Berrin Arserim Muzaffer Aydın Ketani Kasım Aydin Nurdan Karacan–Sever Nida Özcan Burçak Aslan–Çelik |
| author_sort | Filiz Özcan |
| collection | DOAJ |
| description |
The present study aimed to detect Yersinia ruckeri bacteria in fish samples collected from the rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) farms in the South Eastern Anatolia Region of Turkey through bacteriological culturing and polymerase chain reaction. Separate visits were made to trout farming facilities in Diyarbakır, Adıyaman, Şanlıurfa, and Batman in January and February 2021, and 30 fish with an average live weight of 200–250 g were collected from each facility. A total of 1,200 samples were taken from 40 trout farms. Liver, spleen, kidney, and tissue specimens were collected from rainbow trout. Yersinia ruckeri was isolated from the kidney, liver and muscle tissue of fish on McConkey Agar for bacteriological examinations. Isolated strains were identified by MALDİ–TOF and PCR. It was detected the bacteria in the liver, kidney, and muscle tissue samples of fish from 23 out of 40 farms. In conclusion, these bacteria were detected in local farms. These farms must implement vaccination, minimize stress factors affecting the fish, and avoid overstocking. The best methods to protect against infection include avoiding overstocking, low oxygen levels, and underqualified labor force.
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| first_indexed | 2024-03-12T21:14:35Z |
| format | Article |
| id | doaj.art-1ffc63a2a6a14e388132c9e3228c1abe |
| institution | Directory Open Access Journal |
| issn | 0798-2259 2521-9715 |
| language | English |
| last_indexed | 2024-03-12T21:14:35Z |
| publishDate | 2023-07-01 |
| publisher | Universidad del Zulia |
| record_format | Article |
| series | Revista Científica |
| spelling | doaj.art-1ffc63a2a6a14e388132c9e3228c1abe2023-07-29T15:22:08ZengUniversidad del ZuliaRevista Científica0798-22592521-97152023-07-0133210.52973/rcfcv-e33280Detection of Yersinia ruckeri in naturally infected rainbow trout (Oncorhynchus mykiss Walbaum, 1792) from trout farms in Anatolia, TurkeyFiliz Özcan0Neval Berrin Arserim1Muzaffer Aydın Ketani2Kasım Aydin3Nurdan Karacan–Sever4Nida Özcan5Burçak Aslan–Çelik6Dicle University, Veterinary Faculty, Fisheries and Fisheries Diseases Department. Diyarbakır, TurkeyDicle University, Veterinary Faculty, Department of Microbiology. Diyarbakır, TurkeyDicle University, Veterinary Faculty, Department of Histology and Embryology. Diyarbakır, TurkeyMetropolitan Municipality of Diyarbakır. Diyarbakır, TurkeyDicle University, Veterinary Faculty, Department of Microbiology. Diyarbakır, TurkeyDicle University, Medical Faculty, Department of Medical Microbiology. Diyarbakır, TurkeySiirt University, Veterinary Faculty, Department of Parasitology. Siirt, Turkey The present study aimed to detect Yersinia ruckeri bacteria in fish samples collected from the rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) farms in the South Eastern Anatolia Region of Turkey through bacteriological culturing and polymerase chain reaction. Separate visits were made to trout farming facilities in Diyarbakır, Adıyaman, Şanlıurfa, and Batman in January and February 2021, and 30 fish with an average live weight of 200–250 g were collected from each facility. A total of 1,200 samples were taken from 40 trout farms. Liver, spleen, kidney, and tissue specimens were collected from rainbow trout. Yersinia ruckeri was isolated from the kidney, liver and muscle tissue of fish on McConkey Agar for bacteriological examinations. Isolated strains were identified by MALDİ–TOF and PCR. It was detected the bacteria in the liver, kidney, and muscle tissue samples of fish from 23 out of 40 farms. In conclusion, these bacteria were detected in local farms. These farms must implement vaccination, minimize stress factors affecting the fish, and avoid overstocking. The best methods to protect against infection include avoiding overstocking, low oxygen levels, and underqualified labor force. http://www.produccioncientificaluz.org/index.php/cientifica/article/view/40607MALDI–TOFOncorhynchus mykissPCRYersinia ruckeri |
| spellingShingle | Filiz Özcan Neval Berrin Arserim Muzaffer Aydın Ketani Kasım Aydin Nurdan Karacan–Sever Nida Özcan Burçak Aslan–Çelik Detection of Yersinia ruckeri in naturally infected rainbow trout (Oncorhynchus mykiss Walbaum, 1792) from trout farms in Anatolia, Turkey Revista Científica MALDI–TOF Oncorhynchus mykiss PCR Yersinia ruckeri |
| title | Detection of Yersinia ruckeri in naturally infected rainbow trout (Oncorhynchus mykiss Walbaum, 1792) from trout farms in Anatolia, Turkey |
| title_full | Detection of Yersinia ruckeri in naturally infected rainbow trout (Oncorhynchus mykiss Walbaum, 1792) from trout farms in Anatolia, Turkey |
| title_fullStr | Detection of Yersinia ruckeri in naturally infected rainbow trout (Oncorhynchus mykiss Walbaum, 1792) from trout farms in Anatolia, Turkey |
| title_full_unstemmed | Detection of Yersinia ruckeri in naturally infected rainbow trout (Oncorhynchus mykiss Walbaum, 1792) from trout farms in Anatolia, Turkey |
| title_short | Detection of Yersinia ruckeri in naturally infected rainbow trout (Oncorhynchus mykiss Walbaum, 1792) from trout farms in Anatolia, Turkey |
| title_sort | detection of yersinia ruckeri in naturally infected rainbow trout oncorhynchus mykiss walbaum 1792 from trout farms in anatolia turkey |
| topic | MALDI–TOF Oncorhynchus mykiss PCR Yersinia ruckeri |
| url | http://www.produccioncientificaluz.org/index.php/cientifica/article/view/40607 |
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