Analysis of D1853N ATM Polymorphism in Radiosensitive Patients with Cervical Carcinoma
Clinical oncologists have been focusing their efforts on attempting to define risk groups of patients with unusual biological reactions to the recommended therapy regimens using molecular biology techniques. The aims of our study were: (i) to find a design and validate a method for fast and reliable...
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| Format: | Article |
| Language: | English |
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Karolinum Press
2011-01-01
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| Series: | Acta Medica |
| Subjects: | |
| Online Access: | https://actamedica.lfhk.cuni.cz/54/3/0111/ |
| _version_ | 1818298069370798080 |
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| author | Martin Beránek Monika Drastíková Simona Paulíková Igor Sirák Milan Vošmik Jiří Petera |
| author_facet | Martin Beránek Monika Drastíková Simona Paulíková Igor Sirák Milan Vošmik Jiří Petera |
| author_sort | Martin Beránek |
| collection | DOAJ |
| description | Clinical oncologists have been focusing their efforts on attempting to define risk groups of patients with unusual biological reactions to the recommended therapy regimens using molecular biology techniques. The aims of our study were: (i) to find a design and validate a method for fast and reliable analysis of the D1853N (5557G>A) genetic polymorphism in the ATM (ataxia-telangiectasia mutated) gene; (ii) to use side-directed mutagenesis to generate ATM 5557A-positive DNA (reference ATM5557A DNA); and (iii) to analyze a group of patients suffering from cervical carcinoma with adverse responses to radiotherapy. The 5557A variant was found in three of twenty women (15%). Our data show that the prevalence of the 5557A allelic variant in cervical cancer subjects with adverse responses after irradiation probably does not differ from the prevalence common in Caucasians. A larger population study should confirm these preliminary results. |
| first_indexed | 2024-12-13T04:29:28Z |
| format | Article |
| id | doaj.art-20012835f96240ccbd174916fbcf15f9 |
| institution | Directory Open Access Journal |
| issn | 1211-4286 1805-9694 |
| language | English |
| last_indexed | 2024-12-13T04:29:28Z |
| publishDate | 2011-01-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Acta Medica |
| spelling | doaj.art-20012835f96240ccbd174916fbcf15f92022-12-21T23:59:37ZengKarolinum PressActa Medica1211-42861805-96942011-01-0154311111610.14712/18059694.2016.31Analysis of D1853N ATM Polymorphism in Radiosensitive Patients with Cervical CarcinomaMartin Beránek0Monika Drastíková1Simona Paulíková2Igor Sirák3Milan Vošmik4Jiří Petera5Charles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Institute of Clinical Biochemistry and Diagnostics, Czech RepublicCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Institute of Clinical Biochemistry and Diagnostics, Czech RepublicCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Clinical Oncology, Czech RepublicCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Clinical Oncology, Czech RepublicCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Clinical Oncology, Czech RepublicCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Clinical Oncology, Czech RepublicClinical oncologists have been focusing their efforts on attempting to define risk groups of patients with unusual biological reactions to the recommended therapy regimens using molecular biology techniques. The aims of our study were: (i) to find a design and validate a method for fast and reliable analysis of the D1853N (5557G>A) genetic polymorphism in the ATM (ataxia-telangiectasia mutated) gene; (ii) to use side-directed mutagenesis to generate ATM 5557A-positive DNA (reference ATM5557A DNA); and (iii) to analyze a group of patients suffering from cervical carcinoma with adverse responses to radiotherapy. The 5557A variant was found in three of twenty women (15%). Our data show that the prevalence of the 5557A allelic variant in cervical cancer subjects with adverse responses after irradiation probably does not differ from the prevalence common in Caucasians. A larger population study should confirm these preliminary results.https://actamedica.lfhk.cuni.cz/54/3/0111/ATMMutationGenotypingOncologyDiagnosticsTreatment |
| spellingShingle | Martin Beránek Monika Drastíková Simona Paulíková Igor Sirák Milan Vošmik Jiří Petera Analysis of D1853N ATM Polymorphism in Radiosensitive Patients with Cervical Carcinoma Acta Medica ATM Mutation Genotyping Oncology Diagnostics Treatment |
| title | Analysis of D1853N ATM Polymorphism in Radiosensitive Patients with Cervical Carcinoma |
| title_full | Analysis of D1853N ATM Polymorphism in Radiosensitive Patients with Cervical Carcinoma |
| title_fullStr | Analysis of D1853N ATM Polymorphism in Radiosensitive Patients with Cervical Carcinoma |
| title_full_unstemmed | Analysis of D1853N ATM Polymorphism in Radiosensitive Patients with Cervical Carcinoma |
| title_short | Analysis of D1853N ATM Polymorphism in Radiosensitive Patients with Cervical Carcinoma |
| title_sort | analysis of d1853n atm polymorphism in radiosensitive patients with cervical carcinoma |
| topic | ATM Mutation Genotyping Oncology Diagnostics Treatment |
| url | https://actamedica.lfhk.cuni.cz/54/3/0111/ |
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