Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen presenting cell compartment
Dendritic cells (DCs) and monocytes are critical regulators and effectors of innate and adaptive immune responses. Monocyte expansion has been described in many pathological states while monocyte and DC deficiency syndromes are relatively recent additions to the catalogue of human primary immunodef...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2013-12-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00495/full |
| _version_ | 1818322351538831360 |
|---|---|
| author | Laura eJardine Dawn eBarge Ashley eAmes-Draycott Sarah ePagan Sharon eCookson Gavin eSpickett Muzlifah eHaniffa Matthew eCollin Venetia eBigley |
| author_facet | Laura eJardine Dawn eBarge Ashley eAmes-Draycott Sarah ePagan Sharon eCookson Gavin eSpickett Muzlifah eHaniffa Matthew eCollin Venetia eBigley |
| author_sort | Laura eJardine |
| collection | DOAJ |
| description | Dendritic cells (DCs) and monocytes are critical regulators and effectors of innate and adaptive immune responses. Monocyte expansion has been described in many pathological states while monocyte and DC deficiency syndromes are relatively recent additions to the catalogue of human primary immunodeficiency disorders. Clinically applicable screening tests to diagnose and monitor these conditions are lacking. Conventional strategies for identifying human DCs and monocytes have been based on the use of a lineage gate to exclude lymphocytes, thus preventing simultaneous detection of DCs, monocytes and lymphocyte subsets. Here we demonstrate that CD4 is a reliable lineage marker for the human peripheral blood antigen presenting cell compartment that can be used to identify DCs and monocytes in parallel with lymphocytes. Based on this principle, simple modification of a standard lymphocyte phenotyping assay permits simultaneous enumeration of four lymphocyte and five DC/monocyte populations from a single sample. This approach is applicable to clinical samples and facilitates the diagnosis of DC and monocyte disorders in a wide range of clinical settings, including genetic deficiency, neoplasia and inflammation. |
| first_indexed | 2024-12-13T10:55:25Z |
| format | Article |
| id | doaj.art-200580ef476a4305b9f20e1d9c3db32f |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-13T10:55:25Z |
| publishDate | 2013-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-200580ef476a4305b9f20e1d9c3db32f2022-12-21T23:49:35ZengFrontiers Media S.A.Frontiers in Immunology1664-32242013-12-01410.3389/fimmu.2013.0049571035Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen presenting cell compartmentLaura eJardine0Dawn eBarge1Ashley eAmes-Draycott2Sarah ePagan3Sharon eCookson4Gavin eSpickett5Muzlifah eHaniffa6Matthew eCollin7Venetia eBigley8Newcastle UniversityNewcastle upon Tyne Hospitals NHS Foundation TrustNewcastle UniversityNewcastle UniversityNewcastle UniversityNewcastle upon Tyne Hospitals NHS Foundation TrustNewcastle UniversityNewcastle UniversityNewcastle UniversityDendritic cells (DCs) and monocytes are critical regulators and effectors of innate and adaptive immune responses. Monocyte expansion has been described in many pathological states while monocyte and DC deficiency syndromes are relatively recent additions to the catalogue of human primary immunodeficiency disorders. Clinically applicable screening tests to diagnose and monitor these conditions are lacking. Conventional strategies for identifying human DCs and monocytes have been based on the use of a lineage gate to exclude lymphocytes, thus preventing simultaneous detection of DCs, monocytes and lymphocyte subsets. Here we demonstrate that CD4 is a reliable lineage marker for the human peripheral blood antigen presenting cell compartment that can be used to identify DCs and monocytes in parallel with lymphocytes. Based on this principle, simple modification of a standard lymphocyte phenotyping assay permits simultaneous enumeration of four lymphocyte and five DC/monocyte populations from a single sample. This approach is applicable to clinical samples and facilitates the diagnosis of DC and monocyte disorders in a wide range of clinical settings, including genetic deficiency, neoplasia and inflammation.http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00495/fullDendritic CellsFlow CytometryHumansMonocytesimmunodeficiencyCD4 |
| spellingShingle | Laura eJardine Dawn eBarge Ashley eAmes-Draycott Sarah ePagan Sharon eCookson Gavin eSpickett Muzlifah eHaniffa Matthew eCollin Venetia eBigley Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen presenting cell compartment Frontiers in Immunology Dendritic Cells Flow Cytometry Humans Monocytes immunodeficiency CD4 |
| title | Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen presenting cell compartment |
| title_full | Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen presenting cell compartment |
| title_fullStr | Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen presenting cell compartment |
| title_full_unstemmed | Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen presenting cell compartment |
| title_short | Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen presenting cell compartment |
| title_sort | rapid detection of dendritic cell and monocyte disorders using cd4 as a lineage marker of the human peripheral blood antigen presenting cell compartment |
| topic | Dendritic Cells Flow Cytometry Humans Monocytes immunodeficiency CD4 |
| url | http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00495/full |
| work_keys_str_mv | AT lauraejardine rapiddetectionofdendriticcellandmonocytedisordersusingcd4asalineagemarkerofthehumanperipheralbloodantigenpresentingcellcompartment AT dawnebarge rapiddetectionofdendriticcellandmonocytedisordersusingcd4asalineagemarkerofthehumanperipheralbloodantigenpresentingcellcompartment AT ashleyeamesdraycott rapiddetectionofdendriticcellandmonocytedisordersusingcd4asalineagemarkerofthehumanperipheralbloodantigenpresentingcellcompartment AT sarahepagan rapiddetectionofdendriticcellandmonocytedisordersusingcd4asalineagemarkerofthehumanperipheralbloodantigenpresentingcellcompartment AT sharonecookson rapiddetectionofdendriticcellandmonocytedisordersusingcd4asalineagemarkerofthehumanperipheralbloodantigenpresentingcellcompartment AT gavinespickett rapiddetectionofdendriticcellandmonocytedisordersusingcd4asalineagemarkerofthehumanperipheralbloodantigenpresentingcellcompartment AT muzlifahehaniffa rapiddetectionofdendriticcellandmonocytedisordersusingcd4asalineagemarkerofthehumanperipheralbloodantigenpresentingcellcompartment AT matthewecollin rapiddetectionofdendriticcellandmonocytedisordersusingcd4asalineagemarkerofthehumanperipheralbloodantigenpresentingcellcompartment AT venetiaebigley rapiddetectionofdendriticcellandmonocytedisordersusingcd4asalineagemarkerofthehumanperipheralbloodantigenpresentingcellcompartment |