Metabolic Markers Associated with Progression of Type 2 Diabetes Induced by High-Fat Diet and Single Low Dose Streptozotocin in Rats
Science is still searching for readily available, cost-effective biomarkers to assess metabolic disorders occurring before the onset and during the development of type-2 diabetes (T2DM). The aim of the present study was to induce T2DM in rats through a high-fat diet, followed by a single administrat...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/7/431 |
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| author | Maria Andonova Petko Dzhelebov Krastina Trifonova Penka Yonkova Nikola Kostadinov Krasimira Nancheva Veselin Ivanov Krasimira Gospodinova Nikola Nizamov Ilia Tsachev Chavdar Chernev |
| author_facet | Maria Andonova Petko Dzhelebov Krastina Trifonova Penka Yonkova Nikola Kostadinov Krasimira Nancheva Veselin Ivanov Krasimira Gospodinova Nikola Nizamov Ilia Tsachev Chavdar Chernev |
| author_sort | Maria Andonova |
| collection | DOAJ |
| description | Science is still searching for readily available, cost-effective biomarkers to assess metabolic disorders occurring before the onset and during the development of type-2 diabetes (T2DM). The aim of the present study was to induce T2DM in rats through a high-fat diet, followed by a single administration of low dose streptozotocin (STZ), and make an assessment of the development of the disease. The rats were divided into two groups—experimental and control—and were monitored for a period of 10 days. Changes in anthropometric parameters, glucose, insulin, lipids, uric acid, advanced oxidation protein products (AOPP), as well as the histological changes in the liver and pancreas, were recorded. To assess insulin resistance, we used the Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) and beta cell function (HOMA-β) and visceral obesity—adiposity index (AI). The data demonstrate that the increasing values of glucose, HOMA-IR, AI, total cholesterol, triacylglycerols, low- and very-low-density lipoproteins are important markers of the pre-diabetic state. The stable hyperglycemia and increased levels of TC, TG, VLDL, LDL, uric acid and AOPP in experimental rats strongly suggest the development of T2DM. HOMA-IR, HOMA-β, AI, and uric acid are reliable criteria for T2DM in rats. |
| first_indexed | 2024-03-11T00:33:56Z |
| format | Article |
| id | doaj.art-200721f50ae1407293750ffa12512723 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T00:33:56Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-200721f50ae1407293750ffa125127232023-11-18T21:42:28ZengMDPI AGVeterinary Sciences2306-73812023-07-0110743110.3390/vetsci10070431Metabolic Markers Associated with Progression of Type 2 Diabetes Induced by High-Fat Diet and Single Low Dose Streptozotocin in RatsMaria Andonova0Petko Dzhelebov1Krastina Trifonova2Penka Yonkova3Nikola Kostadinov4Krasimira Nancheva5Veselin Ivanov6Krasimira Gospodinova7Nikola Nizamov8Ilia Tsachev9Chavdar Chernev10Department of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, BulgariaDepartment of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, BulgariaDepartment of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, BulgariaDepartment of Veterinary Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, BulgariaDepartment of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, BulgariaClinical Laboratory, University Multiprofile Hospital for Active Treatment “Professor Stoyan Kirkovich”, Stara Zagora 6000, BulgariaDepartment of Social Medicine, Health Management and Disaster Medicine, Faculty of Medicine, Trakia University, Stara Zagora 6000, BulgariaDepartment of Veterinary Microbiology, Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, BulgariaDepartment of Veterinary Microbiology, Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, BulgariaDepartment of Veterinary Microbiology, Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, BulgariaIndependent Researcher, Surrey CR3 5ZD, UKScience is still searching for readily available, cost-effective biomarkers to assess metabolic disorders occurring before the onset and during the development of type-2 diabetes (T2DM). The aim of the present study was to induce T2DM in rats through a high-fat diet, followed by a single administration of low dose streptozotocin (STZ), and make an assessment of the development of the disease. The rats were divided into two groups—experimental and control—and were monitored for a period of 10 days. Changes in anthropometric parameters, glucose, insulin, lipids, uric acid, advanced oxidation protein products (AOPP), as well as the histological changes in the liver and pancreas, were recorded. To assess insulin resistance, we used the Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) and beta cell function (HOMA-β) and visceral obesity—adiposity index (AI). The data demonstrate that the increasing values of glucose, HOMA-IR, AI, total cholesterol, triacylglycerols, low- and very-low-density lipoproteins are important markers of the pre-diabetic state. The stable hyperglycemia and increased levels of TC, TG, VLDL, LDL, uric acid and AOPP in experimental rats strongly suggest the development of T2DM. HOMA-IR, HOMA-β, AI, and uric acid are reliable criteria for T2DM in rats.https://www.mdpi.com/2306-7381/10/7/431ratsdiabetes type 2high-fat dietstreptozotocininsulin resistanceobesity |
| spellingShingle | Maria Andonova Petko Dzhelebov Krastina Trifonova Penka Yonkova Nikola Kostadinov Krasimira Nancheva Veselin Ivanov Krasimira Gospodinova Nikola Nizamov Ilia Tsachev Chavdar Chernev Metabolic Markers Associated with Progression of Type 2 Diabetes Induced by High-Fat Diet and Single Low Dose Streptozotocin in Rats Veterinary Sciences rats diabetes type 2 high-fat diet streptozotocin insulin resistance obesity |
| title | Metabolic Markers Associated with Progression of Type 2 Diabetes Induced by High-Fat Diet and Single Low Dose Streptozotocin in Rats |
| title_full | Metabolic Markers Associated with Progression of Type 2 Diabetes Induced by High-Fat Diet and Single Low Dose Streptozotocin in Rats |
| title_fullStr | Metabolic Markers Associated with Progression of Type 2 Diabetes Induced by High-Fat Diet and Single Low Dose Streptozotocin in Rats |
| title_full_unstemmed | Metabolic Markers Associated with Progression of Type 2 Diabetes Induced by High-Fat Diet and Single Low Dose Streptozotocin in Rats |
| title_short | Metabolic Markers Associated with Progression of Type 2 Diabetes Induced by High-Fat Diet and Single Low Dose Streptozotocin in Rats |
| title_sort | metabolic markers associated with progression of type 2 diabetes induced by high fat diet and single low dose streptozotocin in rats |
| topic | rats diabetes type 2 high-fat diet streptozotocin insulin resistance obesity |
| url | https://www.mdpi.com/2306-7381/10/7/431 |
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