Nanopore Sequencing Technology as an Emerging Tool for Diversity Studies of Plant Organellar Genomes
In this comprehensive review, we explore the significant role that nanopore sequencing technology plays in the study of plant organellar genomes, particularly mitochondrial and chloroplast DNA. To date, the application of nanopore sequencing has led to the successful sequencing of over 100 plant mit...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Diversity |
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| Online Access: | https://www.mdpi.com/1424-2818/16/3/173 |
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| author | Jakub Sawicki Katarzyna Krawczyk Łukasz Paukszto Mateusz Maździarz Mateusz Kurzyński Joanna Szablińska-Piernik Monika Szczecińska |
| author_facet | Jakub Sawicki Katarzyna Krawczyk Łukasz Paukszto Mateusz Maździarz Mateusz Kurzyński Joanna Szablińska-Piernik Monika Szczecińska |
| author_sort | Jakub Sawicki |
| collection | DOAJ |
| description | In this comprehensive review, we explore the significant role that nanopore sequencing technology plays in the study of plant organellar genomes, particularly mitochondrial and chloroplast DNA. To date, the application of nanopore sequencing has led to the successful sequencing of over 100 plant mitochondrial genomes and around 80 chloroplast genomes. These figures not only demonstrate the technology’s robustness but also mark a substantial advancement in the field, highlighting its efficacy in decoding the complex and dynamic nature of these genomes. Nanopore sequencing, known for its long-read capabilities, significantly surpasses traditional sequencing techniques, especially in addressing challenges like structural complexity and sequence repetitiveness in organellar DNA. This review delves into the nuances of nanopore sequencing, elaborating on its benefits compared to conventional methods and the groundbreaking applications it has fostered in plant organellar genomics. While its transformative impact is clear, the technology’s limitations, including error rates and computational requirements, are discussed, alongside potential solutions and prospects for technological refinement. |
| first_indexed | 2024-04-24T18:23:11Z |
| format | Article |
| id | doaj.art-20088cd5827b4641baf965e2b2b701d5 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-04-24T18:23:11Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-20088cd5827b4641baf965e2b2b701d52024-03-27T13:33:49ZengMDPI AGDiversity1424-28182024-03-0116317310.3390/d16030173Nanopore Sequencing Technology as an Emerging Tool for Diversity Studies of Plant Organellar GenomesJakub Sawicki0Katarzyna Krawczyk1Łukasz Paukszto2Mateusz Maździarz3Mateusz Kurzyński4Joanna Szablińska-Piernik5Monika Szczecińska6Department of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-1719 Olsztyn, PolandDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-1719 Olsztyn, PolandDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-1719 Olsztyn, PolandDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-1719 Olsztyn, PolandDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-1719 Olsztyn, PolandDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-1719 Olsztyn, PolandDepartment of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-1719 Olsztyn, PolandIn this comprehensive review, we explore the significant role that nanopore sequencing technology plays in the study of plant organellar genomes, particularly mitochondrial and chloroplast DNA. To date, the application of nanopore sequencing has led to the successful sequencing of over 100 plant mitochondrial genomes and around 80 chloroplast genomes. These figures not only demonstrate the technology’s robustness but also mark a substantial advancement in the field, highlighting its efficacy in decoding the complex and dynamic nature of these genomes. Nanopore sequencing, known for its long-read capabilities, significantly surpasses traditional sequencing techniques, especially in addressing challenges like structural complexity and sequence repetitiveness in organellar DNA. This review delves into the nuances of nanopore sequencing, elaborating on its benefits compared to conventional methods and the groundbreaking applications it has fostered in plant organellar genomics. While its transformative impact is clear, the technology’s limitations, including error rates and computational requirements, are discussed, alongside potential solutions and prospects for technological refinement.https://www.mdpi.com/1424-2818/16/3/173plastomeplant mitogenomenanopore sequencingtarget enrichmentadaptive sampling |
| spellingShingle | Jakub Sawicki Katarzyna Krawczyk Łukasz Paukszto Mateusz Maździarz Mateusz Kurzyński Joanna Szablińska-Piernik Monika Szczecińska Nanopore Sequencing Technology as an Emerging Tool for Diversity Studies of Plant Organellar Genomes Diversity plastome plant mitogenome nanopore sequencing target enrichment adaptive sampling |
| title | Nanopore Sequencing Technology as an Emerging Tool for Diversity Studies of Plant Organellar Genomes |
| title_full | Nanopore Sequencing Technology as an Emerging Tool for Diversity Studies of Plant Organellar Genomes |
| title_fullStr | Nanopore Sequencing Technology as an Emerging Tool for Diversity Studies of Plant Organellar Genomes |
| title_full_unstemmed | Nanopore Sequencing Technology as an Emerging Tool for Diversity Studies of Plant Organellar Genomes |
| title_short | Nanopore Sequencing Technology as an Emerging Tool for Diversity Studies of Plant Organellar Genomes |
| title_sort | nanopore sequencing technology as an emerging tool for diversity studies of plant organellar genomes |
| topic | plastome plant mitogenome nanopore sequencing target enrichment adaptive sampling |
| url | https://www.mdpi.com/1424-2818/16/3/173 |
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