Advancements in CRISPR-Based Biosensing for Next-Gen Point of Care Diagnostic Application
With the move of molecular tests from diagnostic labs to on-site testing becoming more common, there is a sudden rise in demand for nucleic acid-based diagnostic tools that are selective, sensitive, flexible to terrain changes, and cost-effective to assist in point-of-care systems for large-scale sc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/2/202 |
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| author | Akash Kumaran Nathan Jude Serpes Tisha Gupta Abija James Avinash Sharma Deepak Kumar Rupak Nagraik Vaneet Kumar Sadanand Pandey |
| author_facet | Akash Kumaran Nathan Jude Serpes Tisha Gupta Abija James Avinash Sharma Deepak Kumar Rupak Nagraik Vaneet Kumar Sadanand Pandey |
| author_sort | Akash Kumaran |
| collection | DOAJ |
| description | With the move of molecular tests from diagnostic labs to on-site testing becoming more common, there is a sudden rise in demand for nucleic acid-based diagnostic tools that are selective, sensitive, flexible to terrain changes, and cost-effective to assist in point-of-care systems for large-scale screening and to be used in remote locations in cases of outbreaks and pandemics. CRISPR-based biosensors comprise a promising new approach to nucleic acid detection, which uses Cas effector proteins (Cas9, Cas12, and Cas13) as extremely specialized identification components that may be used in conjunction with a variety of readout approaches (such as fluorescence, colorimetry, potentiometry, lateral flow assay, etc.) for onsite analysis. In this review, we cover some technical aspects of integrating the CRISPR Cas system with traditional biosensing readout methods and amplification technologies such as polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), and recombinase polymerase amplification (RPA) and continue to elaborate on the prospects of the developed biosensor in the detection of some major viral and bacterial diseases. Within the scope of this article, we also discuss the recent COVID pandemic and the numerous CRISPR biosensors that have undergone development since its advent. Finally, we discuss some challenges and future prospects of CRISPR Cas systems in point-of-care testing. |
| first_indexed | 2024-03-11T09:04:44Z |
| format | Article |
| id | doaj.art-200a76fe97db4a50b7d5dd842b1c9320 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T09:04:44Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-200a76fe97db4a50b7d5dd842b1c93202023-11-16T19:25:29ZengMDPI AGBiosensors2079-63742023-01-0113220210.3390/bios13020202Advancements in CRISPR-Based Biosensing for Next-Gen Point of Care Diagnostic ApplicationAkash Kumaran0Nathan Jude Serpes1Tisha Gupta2Abija James3Avinash Sharma4Deepak Kumar5Rupak Nagraik6Vaneet Kumar7Sadanand Pandey8Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan 173229, Himachal Pradesh, IndiaFaculty of Applied Sciences and Biotechnology, Shoolini University, Solan 173229, Himachal Pradesh, IndiaFaculty of Applied Sciences and Biotechnology, Shoolini University, Solan 173229, Himachal Pradesh, IndiaFaculty of Applied Sciences and Biotechnology, Shoolini University, Solan 173229, Himachal Pradesh, IndiaFaculty of Applied Sciences and Biotechnology, Shoolini University, Solan 173229, Himachal Pradesh, IndiaDepartment of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Shoolini University, Solan 173229, Himachal Pradesh, IndiaFaculty of Applied Sciences and Biotechnology, Shoolini University, Solan 173229, Himachal Pradesh, IndiaDepartment of Natural Science, CT University, Ludhiana 142024, Punjab, IndiaDepartment of Chemistry, College of Natural Sciences, Yeungnam University, Gyeongsan 38541, Gyeongbuk, Republic of KoreaWith the move of molecular tests from diagnostic labs to on-site testing becoming more common, there is a sudden rise in demand for nucleic acid-based diagnostic tools that are selective, sensitive, flexible to terrain changes, and cost-effective to assist in point-of-care systems for large-scale screening and to be used in remote locations in cases of outbreaks and pandemics. CRISPR-based biosensors comprise a promising new approach to nucleic acid detection, which uses Cas effector proteins (Cas9, Cas12, and Cas13) as extremely specialized identification components that may be used in conjunction with a variety of readout approaches (such as fluorescence, colorimetry, potentiometry, lateral flow assay, etc.) for onsite analysis. In this review, we cover some technical aspects of integrating the CRISPR Cas system with traditional biosensing readout methods and amplification technologies such as polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), and recombinase polymerase amplification (RPA) and continue to elaborate on the prospects of the developed biosensor in the detection of some major viral and bacterial diseases. Within the scope of this article, we also discuss the recent COVID pandemic and the numerous CRISPR biosensors that have undergone development since its advent. Finally, we discuss some challenges and future prospects of CRISPR Cas systems in point-of-care testing.https://www.mdpi.com/2079-6374/13/2/202CRISPRCas effector proteinsbiosensordiseasesE-CRISPR |
| spellingShingle | Akash Kumaran Nathan Jude Serpes Tisha Gupta Abija James Avinash Sharma Deepak Kumar Rupak Nagraik Vaneet Kumar Sadanand Pandey Advancements in CRISPR-Based Biosensing for Next-Gen Point of Care Diagnostic Application Biosensors CRISPR Cas effector proteins biosensor diseases E-CRISPR |
| title | Advancements in CRISPR-Based Biosensing for Next-Gen Point of Care Diagnostic Application |
| title_full | Advancements in CRISPR-Based Biosensing for Next-Gen Point of Care Diagnostic Application |
| title_fullStr | Advancements in CRISPR-Based Biosensing for Next-Gen Point of Care Diagnostic Application |
| title_full_unstemmed | Advancements in CRISPR-Based Biosensing for Next-Gen Point of Care Diagnostic Application |
| title_short | Advancements in CRISPR-Based Biosensing for Next-Gen Point of Care Diagnostic Application |
| title_sort | advancements in crispr based biosensing for next gen point of care diagnostic application |
| topic | CRISPR Cas effector proteins biosensor diseases E-CRISPR |
| url | https://www.mdpi.com/2079-6374/13/2/202 |
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