Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system
We present a novel in vitro model in which to investigate the efficacy of experimental drugs for the promotion of axon regeneration in the central nervous system. We co-cultured rat hippocampal neurons and cerebral cortical oligodendrocytes, and tested the co-culture system using a Nogo-66 receptor...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=10;spage=1612;epage=1616;aulast=Gang |
| _version_ | 1818195379718455296 |
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| author | Lin Gang Yu-chen Yao Ying-fu Liu Yi-peng Li Kai Yang Lei Lu Yuan-chi Cheng Xu-yi Chen Yue Tu |
| author_facet | Lin Gang Yu-chen Yao Ying-fu Liu Yi-peng Li Kai Yang Lei Lu Yuan-chi Cheng Xu-yi Chen Yue Tu |
| author_sort | Lin Gang |
| collection | DOAJ |
| description | We present a novel in vitro model in which to investigate the efficacy of experimental drugs for the promotion of axon regeneration in the central nervous system. We co-cultured rat hippocampal neurons and cerebral cortical oligodendrocytes, and tested the co-culture system using a Nogo-66 receptor antagonist peptide (NEP1-40), which promotes axonal growth. Primary cultured oligodendrocytes suppressed axonal growth in the rat hippocampus, but NEP1-40 stimulated axonal growth in the co-culture system. Our results confirm the validity of the neuron-oligodendrocyte co-culture system as an assay for the evaluation of drugs for axon regeneration in the central nervous system. |
| first_indexed | 2024-12-12T01:17:15Z |
| format | Article |
| id | doaj.art-200ab79b598547cdbf02f5ce4a0bdebd |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-12T01:17:15Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-200ab79b598547cdbf02f5ce4a0bdebd2022-12-22T00:43:19ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742015-01-0110101612161610.4103/1673-5374.167759Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous systemLin GangYu-chen YaoYing-fu LiuYi-peng LiKai YangLei LuYuan-chi ChengXu-yi ChenYue TuWe present a novel in vitro model in which to investigate the efficacy of experimental drugs for the promotion of axon regeneration in the central nervous system. We co-cultured rat hippocampal neurons and cerebral cortical oligodendrocytes, and tested the co-culture system using a Nogo-66 receptor antagonist peptide (NEP1-40), which promotes axonal growth. Primary cultured oligodendrocytes suppressed axonal growth in the rat hippocampus, but NEP1-40 stimulated axonal growth in the co-culture system. Our results confirm the validity of the neuron-oligodendrocyte co-culture system as an assay for the evaluation of drugs for axon regeneration in the central nervous system.http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=10;spage=1612;epage=1616;aulast=Gangnerve regeneration; experimental models; NEP1-40; oligodendrocytes; neurons; axon regeneration; Nogo; PC12 cells; neural regeneration |
| spellingShingle | Lin Gang Yu-chen Yao Ying-fu Liu Yi-peng Li Kai Yang Lei Lu Yuan-chi Cheng Xu-yi Chen Yue Tu Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system Neural Regeneration Research nerve regeneration; experimental models; NEP1-40; oligodendrocytes; neurons; axon regeneration; Nogo; PC12 cells; neural regeneration |
| title | Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system |
| title_full | Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system |
| title_fullStr | Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system |
| title_full_unstemmed | Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system |
| title_short | Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system |
| title_sort | co culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system |
| topic | nerve regeneration; experimental models; NEP1-40; oligodendrocytes; neurons; axon regeneration; Nogo; PC12 cells; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=10;spage=1612;epage=1616;aulast=Gang |
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