The commercial pig as a model of spontaneously-occurring osteoarthritis
Abstract Background Preclinical osteoarthritis models where damage occurs spontaneously may better reflect the initiation and development of human osteoarthritis. The aim was to assess the commercial pig as a model of spontaneous osteoarthritis development by examining pain-associated behaviour, joi...
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| Format: | Article |
| Language: | English |
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BMC
2019-02-01
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| Series: | BMC Musculoskeletal Disorders |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12891-019-2452-0 |
| _version_ | 1818277121549664256 |
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| author | Mhairi A. Macfadyen Zoe Daniel Sara Kelly Tim Parr John M. Brameld Andrew J. Murton Simon W. Jones |
| author_facet | Mhairi A. Macfadyen Zoe Daniel Sara Kelly Tim Parr John M. Brameld Andrew J. Murton Simon W. Jones |
| author_sort | Mhairi A. Macfadyen |
| collection | DOAJ |
| description | Abstract Background Preclinical osteoarthritis models where damage occurs spontaneously may better reflect the initiation and development of human osteoarthritis. The aim was to assess the commercial pig as a model of spontaneous osteoarthritis development by examining pain-associated behaviour, joint cartilage integrity, as well as the use of porcine cartilage explants and isolated chondrocytes and osteoblasts for ex vivo and in vitro studies. Methods Female pigs (Large white x Landrace x Duroc) were examined at different ages from 6 weeks to 3–4 years old. Lameness was assessed as a marker of pain-associated behaviour. Femorotibial joint cartilage integrity was determined by chondropathy scoring and histological staining of proteoglycan. IL-6 production and proteoglycan degradation was assessed in cartilage explants and primary porcine chondrocytes by ELISA and DMMB assay. Primary porcine osteoblasts from damaged and non-damaged joints, as determined by chondropathy scoring, were assessed for mineralisation, proliferative and mitochondrial function as a marker of metabolic capacity. Results Pigs aged 80 weeks and older exhibited lameness. Osteoarthritic lesions in femoral condyle and tibial plateau cartilage were apparent from 40 weeks and increased in severity with age up to 3–4 years old. Cartilage from damaged joints exhibited proteoglycan loss, which positively correlated with chondropathy score. Stimulation of porcine cartilage explants and primary chondrocytes with either IL-1β or visfatin induced IL-6 production and proteoglycan degradation. Primary porcine osteoblasts from damaged joints exhibited reduced proliferative, mineralisation, and metabolic capacity. Conclusion In conclusion, the commercial pig represents an alternative model of spontaneous osteoarthritis and an excellent source of tissue for in vitro and ex vivo studies. |
| first_indexed | 2024-12-12T22:56:30Z |
| format | Article |
| id | doaj.art-200b0c7c84724efabd63898558c07d43 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-12T22:56:30Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-200b0c7c84724efabd63898558c07d432022-12-22T00:08:56ZengBMCBMC Musculoskeletal Disorders1471-24742019-02-0120111210.1186/s12891-019-2452-0The commercial pig as a model of spontaneously-occurring osteoarthritisMhairi A. Macfadyen0Zoe Daniel1Sara Kelly2Tim Parr3John M. Brameld4Andrew J. Murton5Simon W. Jones6MRC-ARUK Centre for Musculoskeletal Ageing Research, School of Biosciences, University of NottinghamMRC-ARUK Centre for Musculoskeletal Ageing Research, School of Biosciences, University of NottinghamMRC-ARUK Centre for Musculoskeletal Ageing Research, School of Biosciences, University of NottinghamMRC-ARUK Centre for Musculoskeletal Ageing Research, School of Biosciences, University of NottinghamMRC-ARUK Centre for Musculoskeletal Ageing Research, School of Biosciences, University of NottinghamMRC-ARUK Centre for Musculoskeletal Ageing Research, School of Biosciences, University of NottinghamInstitute of Inflammation and Ageing, MRC-ARUK Centre for Musculoskeletal Ageing Research, School of Immunity, University of BirminghamAbstract Background Preclinical osteoarthritis models where damage occurs spontaneously may better reflect the initiation and development of human osteoarthritis. The aim was to assess the commercial pig as a model of spontaneous osteoarthritis development by examining pain-associated behaviour, joint cartilage integrity, as well as the use of porcine cartilage explants and isolated chondrocytes and osteoblasts for ex vivo and in vitro studies. Methods Female pigs (Large white x Landrace x Duroc) were examined at different ages from 6 weeks to 3–4 years old. Lameness was assessed as a marker of pain-associated behaviour. Femorotibial joint cartilage integrity was determined by chondropathy scoring and histological staining of proteoglycan. IL-6 production and proteoglycan degradation was assessed in cartilage explants and primary porcine chondrocytes by ELISA and DMMB assay. Primary porcine osteoblasts from damaged and non-damaged joints, as determined by chondropathy scoring, were assessed for mineralisation, proliferative and mitochondrial function as a marker of metabolic capacity. Results Pigs aged 80 weeks and older exhibited lameness. Osteoarthritic lesions in femoral condyle and tibial plateau cartilage were apparent from 40 weeks and increased in severity with age up to 3–4 years old. Cartilage from damaged joints exhibited proteoglycan loss, which positively correlated with chondropathy score. Stimulation of porcine cartilage explants and primary chondrocytes with either IL-1β or visfatin induced IL-6 production and proteoglycan degradation. Primary porcine osteoblasts from damaged joints exhibited reduced proliferative, mineralisation, and metabolic capacity. Conclusion In conclusion, the commercial pig represents an alternative model of spontaneous osteoarthritis and an excellent source of tissue for in vitro and ex vivo studies.http://link.springer.com/article/10.1186/s12891-019-2452-0OsteoarthritisOsteoblastChondropathyPigChondrocyte |
| spellingShingle | Mhairi A. Macfadyen Zoe Daniel Sara Kelly Tim Parr John M. Brameld Andrew J. Murton Simon W. Jones The commercial pig as a model of spontaneously-occurring osteoarthritis BMC Musculoskeletal Disorders Osteoarthritis Osteoblast Chondropathy Pig Chondrocyte |
| title | The commercial pig as a model of spontaneously-occurring osteoarthritis |
| title_full | The commercial pig as a model of spontaneously-occurring osteoarthritis |
| title_fullStr | The commercial pig as a model of spontaneously-occurring osteoarthritis |
| title_full_unstemmed | The commercial pig as a model of spontaneously-occurring osteoarthritis |
| title_short | The commercial pig as a model of spontaneously-occurring osteoarthritis |
| title_sort | commercial pig as a model of spontaneously occurring osteoarthritis |
| topic | Osteoarthritis Osteoblast Chondropathy Pig Chondrocyte |
| url | http://link.springer.com/article/10.1186/s12891-019-2452-0 |
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