Guidance of Drosophila Mushroom Body Axons Depends upon DRL-Wnt Receptor Cleavage in the Brain Dorsomedial Lineage Precursors
In vivo axon pathfinding mechanisms in the neuron-dense brain remain relatively poorly characterized. We study the Drosophila mushroom body (MB) axons, whose α and β branches connect to different brain areas. We show that the Ryk family WNT5 receptor, DRL (derailed), which is expressed in the dorsom...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-05-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124715004362 |
| _version_ | 1818336460743376896 |
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| author | Elodie Reynaud Liza L. Lahaye Ana Boulanger Iveta M. Petrova Claire Marquilly Adrien Flandre Tania Martianez Martin Privat Jasprina N. Noordermeer Lee G. Fradkin Jean-Maurice Dura |
| author_facet | Elodie Reynaud Liza L. Lahaye Ana Boulanger Iveta M. Petrova Claire Marquilly Adrien Flandre Tania Martianez Martin Privat Jasprina N. Noordermeer Lee G. Fradkin Jean-Maurice Dura |
| author_sort | Elodie Reynaud |
| collection | DOAJ |
| description | In vivo axon pathfinding mechanisms in the neuron-dense brain remain relatively poorly characterized. We study the Drosophila mushroom body (MB) axons, whose α and β branches connect to different brain areas. We show that the Ryk family WNT5 receptor, DRL (derailed), which is expressed in the dorsomedial lineages, brain structure precursors adjacent to the MBs, is required for MB α branch axon guidance. DRL acts to capture and present WNT5 to MB axons rather than transduce a WNT5 signal. DRL’s ectodomain must be cleaved and shed to guide α axons. DRL-2, another Ryk, is expressed within MB axons and functions as a repulsive WNT5 signaling receptor. Finally, our biochemical data support the existence of a ternary complex composed of the cleaved DRL ectodomain, WNT5, and DRL-2. Thus, the interaction of MB-extrinsic and -intrinsic Ryks via their common ligand acts to guide MB α axons. |
| first_indexed | 2024-12-13T14:39:41Z |
| format | Article |
| id | doaj.art-200f617be84d40088ed02af0586f1252 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-13T14:39:41Z |
| publishDate | 2015-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-200f617be84d40088ed02af0586f12522022-12-21T23:41:38ZengElsevierCell Reports2211-12472015-05-011181293130410.1016/j.celrep.2015.04.035Guidance of Drosophila Mushroom Body Axons Depends upon DRL-Wnt Receptor Cleavage in the Brain Dorsomedial Lineage PrecursorsElodie Reynaud0Liza L. Lahaye1Ana Boulanger2Iveta M. Petrova3Claire Marquilly4Adrien Flandre5Tania Martianez6Martin Privat7Jasprina N. Noordermeer8Lee G. Fradkin9Jean-Maurice Dura10Institute of Human Genetics, UPR1142, CNRS, 141, rue de la Cardonille, 34396 Montpellier, FranceDepartment of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, the NetherlandsInstitute of Human Genetics, UPR1142, CNRS, 141, rue de la Cardonille, 34396 Montpellier, FranceDepartment of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, the NetherlandsInstitute of Human Genetics, UPR1142, CNRS, 141, rue de la Cardonille, 34396 Montpellier, FranceInstitute of Human Genetics, UPR1142, CNRS, 141, rue de la Cardonille, 34396 Montpellier, FranceDepartment of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, the NetherlandsInstitute of Human Genetics, UPR1142, CNRS, 141, rue de la Cardonille, 34396 Montpellier, FranceDepartment of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, the NetherlandsDepartment of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, the NetherlandsInstitute of Human Genetics, UPR1142, CNRS, 141, rue de la Cardonille, 34396 Montpellier, FranceIn vivo axon pathfinding mechanisms in the neuron-dense brain remain relatively poorly characterized. We study the Drosophila mushroom body (MB) axons, whose α and β branches connect to different brain areas. We show that the Ryk family WNT5 receptor, DRL (derailed), which is expressed in the dorsomedial lineages, brain structure precursors adjacent to the MBs, is required for MB α branch axon guidance. DRL acts to capture and present WNT5 to MB axons rather than transduce a WNT5 signal. DRL’s ectodomain must be cleaved and shed to guide α axons. DRL-2, another Ryk, is expressed within MB axons and functions as a repulsive WNT5 signaling receptor. Finally, our biochemical data support the existence of a ternary complex composed of the cleaved DRL ectodomain, WNT5, and DRL-2. Thus, the interaction of MB-extrinsic and -intrinsic Ryks via their common ligand acts to guide MB α axons.http://www.sciencedirect.com/science/article/pii/S2211124715004362 |
| spellingShingle | Elodie Reynaud Liza L. Lahaye Ana Boulanger Iveta M. Petrova Claire Marquilly Adrien Flandre Tania Martianez Martin Privat Jasprina N. Noordermeer Lee G. Fradkin Jean-Maurice Dura Guidance of Drosophila Mushroom Body Axons Depends upon DRL-Wnt Receptor Cleavage in the Brain Dorsomedial Lineage Precursors Cell Reports |
| title | Guidance of Drosophila Mushroom Body Axons Depends upon DRL-Wnt Receptor Cleavage in the Brain Dorsomedial Lineage Precursors |
| title_full | Guidance of Drosophila Mushroom Body Axons Depends upon DRL-Wnt Receptor Cleavage in the Brain Dorsomedial Lineage Precursors |
| title_fullStr | Guidance of Drosophila Mushroom Body Axons Depends upon DRL-Wnt Receptor Cleavage in the Brain Dorsomedial Lineage Precursors |
| title_full_unstemmed | Guidance of Drosophila Mushroom Body Axons Depends upon DRL-Wnt Receptor Cleavage in the Brain Dorsomedial Lineage Precursors |
| title_short | Guidance of Drosophila Mushroom Body Axons Depends upon DRL-Wnt Receptor Cleavage in the Brain Dorsomedial Lineage Precursors |
| title_sort | guidance of drosophila mushroom body axons depends upon drl wnt receptor cleavage in the brain dorsomedial lineage precursors |
| url | http://www.sciencedirect.com/science/article/pii/S2211124715004362 |
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