The Cell Wall-Derived Xyloglucan Is a New DAMP Triggering Plant Immunity in Vitis vinifera and Arabidopsis thaliana
Damage-associated molecular patterns (DAMPs) are endogenous molecules that can activate the plant innate immunity. DAMPs can derive from the plant cell wall, which is composed of a complex mixture of cellulose, hemicellulose, and pectin polysaccharides. Fragments of pectin, called oligogalacturonide...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.01725/full |
| _version_ | 1819027508083818496 |
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| author | Justine Claverie Suzanne Balacey Christelle Lemaître-Guillier Daphnée Brulé Annick Chiltz Lucie Granet Elodie Noirot Xavier Daire Benoît Darblade Marie-Claire Héloir Benoit Poinssot |
| author_facet | Justine Claverie Suzanne Balacey Christelle Lemaître-Guillier Daphnée Brulé Annick Chiltz Lucie Granet Elodie Noirot Xavier Daire Benoît Darblade Marie-Claire Héloir Benoit Poinssot |
| author_sort | Justine Claverie |
| collection | DOAJ |
| description | Damage-associated molecular patterns (DAMPs) are endogenous molecules that can activate the plant innate immunity. DAMPs can derive from the plant cell wall, which is composed of a complex mixture of cellulose, hemicellulose, and pectin polysaccharides. Fragments of pectin, called oligogalacturonides (OG), can be released after wounding or by pathogen-encoded cell wall degrading enzymes (CWDEs) such as polygalacturonases (PGs). OG are known to induce innate immune responses, including the activation of mitogen-activated protein kinases (MAPKs), production of H2O2, defense gene activation, and callose deposition. Thus, we hypothesized that xyloglucans (Xh), derived from the plant cell wall hemicellulose, could also act as an endogenous elicitor and trigger a signaling cascade similar to OG. Our results indicate that purified Xh elicit MAPK activation and immune gene expression in grapevine (Vitis vinifera) and Arabidopsis (Arabidopsis thaliana) to trigger induced resistance against necrotrophic (Botrytis cinerea) or biotrophic (Hyaloperonospora arabidopsidis) pathogens. Xh also induce resveratrol production in grapevine cell suspension and callose deposition in Arabidopsis which depends on the callose synthase PMR4. In addition, we characterized some signaling components of Xh-induced immunity using Arabidopsis mutants. Our data suggest that Xh-induced resistance against B. cinerea is dependent on the phytoalexin, salicylate, jasmonate, and ethylene pathways. |
| first_indexed | 2024-12-21T05:43:35Z |
| format | Article |
| id | doaj.art-20128bb36a704654a382a3455d820140 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T05:43:35Z |
| publishDate | 2018-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-20128bb36a704654a382a3455d8201402022-12-21T19:14:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-11-01910.3389/fpls.2018.01725419889The Cell Wall-Derived Xyloglucan Is a New DAMP Triggering Plant Immunity in Vitis vinifera and Arabidopsis thalianaJustine Claverie0Suzanne Balacey1Christelle Lemaître-Guillier2Daphnée Brulé3Annick Chiltz4Lucie Granet5Elodie Noirot6Xavier Daire7Benoît Darblade8Marie-Claire Héloir9Benoit Poinssot10Agroécologie, Agrosup Dijon, INRA, Université Bourgogne Franche-Comté, CNRS ERL, Dijon, FranceAgroécologie, Agrosup Dijon, INRA, Université Bourgogne Franche-Comté, CNRS ERL, Dijon, FranceAgroécologie, Agrosup Dijon, INRA, Université Bourgogne Franche-Comté, CNRS ERL, Dijon, FranceAgroécologie, Agrosup Dijon, INRA, Université Bourgogne Franche-Comté, CNRS ERL, Dijon, FranceAgroécologie, Agrosup Dijon, INRA, Université Bourgogne Franche-Comté, CNRS ERL, Dijon, FranceAgroécologie, Agrosup Dijon, INRA, Université Bourgogne Franche-Comté, CNRS ERL, Dijon, FranceAgroécologie, Agrosup Dijon, INRA, Université Bourgogne Franche-Comté, CNRS ERL, Dijon, FranceAgroécologie, Agrosup Dijon, INRA, Université Bourgogne Franche-Comté, CNRS ERL, Dijon, FranceElicityl, Crolles, FranceAgroécologie, Agrosup Dijon, INRA, Université Bourgogne Franche-Comté, CNRS ERL, Dijon, FranceAgroécologie, Agrosup Dijon, INRA, Université Bourgogne Franche-Comté, CNRS ERL, Dijon, FranceDamage-associated molecular patterns (DAMPs) are endogenous molecules that can activate the plant innate immunity. DAMPs can derive from the plant cell wall, which is composed of a complex mixture of cellulose, hemicellulose, and pectin polysaccharides. Fragments of pectin, called oligogalacturonides (OG), can be released after wounding or by pathogen-encoded cell wall degrading enzymes (CWDEs) such as polygalacturonases (PGs). OG are known to induce innate immune responses, including the activation of mitogen-activated protein kinases (MAPKs), production of H2O2, defense gene activation, and callose deposition. Thus, we hypothesized that xyloglucans (Xh), derived from the plant cell wall hemicellulose, could also act as an endogenous elicitor and trigger a signaling cascade similar to OG. Our results indicate that purified Xh elicit MAPK activation and immune gene expression in grapevine (Vitis vinifera) and Arabidopsis (Arabidopsis thaliana) to trigger induced resistance against necrotrophic (Botrytis cinerea) or biotrophic (Hyaloperonospora arabidopsidis) pathogens. Xh also induce resveratrol production in grapevine cell suspension and callose deposition in Arabidopsis which depends on the callose synthase PMR4. In addition, we characterized some signaling components of Xh-induced immunity using Arabidopsis mutants. Our data suggest that Xh-induced resistance against B. cinerea is dependent on the phytoalexin, salicylate, jasmonate, and ethylene pathways.https://www.frontiersin.org/article/10.3389/fpls.2018.01725/fullDAMP-triggered immunityinduced resistancemitogen-activated protein kinaseBotrytis cinereaelicitor |
| spellingShingle | Justine Claverie Suzanne Balacey Christelle Lemaître-Guillier Daphnée Brulé Annick Chiltz Lucie Granet Elodie Noirot Xavier Daire Benoît Darblade Marie-Claire Héloir Benoit Poinssot The Cell Wall-Derived Xyloglucan Is a New DAMP Triggering Plant Immunity in Vitis vinifera and Arabidopsis thaliana Frontiers in Plant Science DAMP-triggered immunity induced resistance mitogen-activated protein kinase Botrytis cinerea elicitor |
| title | The Cell Wall-Derived Xyloglucan Is a New DAMP Triggering Plant Immunity in Vitis vinifera and Arabidopsis thaliana |
| title_full | The Cell Wall-Derived Xyloglucan Is a New DAMP Triggering Plant Immunity in Vitis vinifera and Arabidopsis thaliana |
| title_fullStr | The Cell Wall-Derived Xyloglucan Is a New DAMP Triggering Plant Immunity in Vitis vinifera and Arabidopsis thaliana |
| title_full_unstemmed | The Cell Wall-Derived Xyloglucan Is a New DAMP Triggering Plant Immunity in Vitis vinifera and Arabidopsis thaliana |
| title_short | The Cell Wall-Derived Xyloglucan Is a New DAMP Triggering Plant Immunity in Vitis vinifera and Arabidopsis thaliana |
| title_sort | cell wall derived xyloglucan is a new damp triggering plant immunity in vitis vinifera and arabidopsis thaliana |
| topic | DAMP-triggered immunity induced resistance mitogen-activated protein kinase Botrytis cinerea elicitor |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.01725/full |
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