Development of a ferroptosis-based molecular markers for predicting RFS in prostate cancer patients
Abstract The goal of this study was to develop a ferroptosis-based molecular signature that can predict recurrence-free survival (RFS) in patients with prostate cancer (PCa). In this study, we obtained ferroptosis-related genes (FRGs) in FerrDb database and clinical transcriptome data in TCGA databa...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-50205-1 |
| _version_ | 1827399510963257344 |
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| author | Jinquan Chen Longbin Zhang Yiling Luo Chao Tan Huang Hu Yuling Jiang Na Xi Qinghai Zeng H. Peng |
| author_facet | Jinquan Chen Longbin Zhang Yiling Luo Chao Tan Huang Hu Yuling Jiang Na Xi Qinghai Zeng H. Peng |
| author_sort | Jinquan Chen |
| collection | DOAJ |
| description | Abstract The goal of this study was to develop a ferroptosis-based molecular signature that can predict recurrence-free survival (RFS) in patients with prostate cancer (PCa). In this study, we obtained ferroptosis-related genes (FRGs) in FerrDb database and clinical transcriptome data in TCGA database and GEO database. Consensus cluster analysis was used to identify three molecular markers of ferroptosis in PCa with differential expression of 40 FRGs, including PD-L1 expression levels. We conducted a new ferroptosis-related signature for PCa RFS using four FRGs identified through univariate and multivariate Cox regression analyses. The signature was validated in the training, testing, and validation cohorts, and it demonstrated remarkable results in the area under the time-dependent receiver operating characteristic (ROC) curve of 0.757, 0.715, and 0.732, respectively. Additionally, we observed that younger patients, those with stage T III and stage T IV, stage N0, cluster 1, and cluster 2 PCa were more accurately predicted by the signature as independent predictors of RFS. DU-145 and RWPE-1 cells were successfully analyzed by qRT-PCR and Western blot for ASNS, GPT2, RRM2, and NFE2L2. In summary, we developed a novel ferroptosis-based signature for RFS in PC, utilizing four FRGs identified through univariate and multivariate Cox regression analyses. This signature was rigorously validated across training, testing, and validation cohorts, demonstrating exceptional performance as evidenced by its ROC curves. Notably, our findings indicate that this signature is particularly effective as an independent predictor of RFS in younger patients or those with stage T III and T IV, stage N0, and in clusters 1 and 2. Finally, we confirmed the expression of these four FRGs in DU-145 and RWPE-1 cell lines. |
| first_indexed | 2024-03-08T19:47:06Z |
| format | Article |
| id | doaj.art-20152251f24e41debc7b388955bcaa4c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T19:47:06Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-20152251f24e41debc7b388955bcaa4c2023-12-24T12:15:45ZengNature PortfolioScientific Reports2045-23222023-12-0113111010.1038/s41598-023-50205-1Development of a ferroptosis-based molecular markers for predicting RFS in prostate cancer patientsJinquan Chen0Longbin Zhang1Yiling Luo2Chao Tan3Huang Hu4Yuling Jiang5Na Xi6Qinghai Zeng7H. Peng8Department of Ophthalmology, The Tongnan District People’s HospitalDepartment of Ophthalmology, The Tongnan District People’s HospitalDepartment of Ophthalmology, The Tongnan District People’s HospitalDepartment of Ophthalmology, The Tongnan District People’s HospitalDepartment of Ophthalmology, The Tongnan District People’s HospitalDepartment of Ophthalmology, The Tongnan District People’s HospitalDepartment of Ophthalmology, The Tongnan District People’s HospitalDepartment of Ophthalmology, The Tongnan District People’s HospitalDepartment of Ophthalmology, The First Affiliated Hospital of Chongqing Medical UniversityAbstract The goal of this study was to develop a ferroptosis-based molecular signature that can predict recurrence-free survival (RFS) in patients with prostate cancer (PCa). In this study, we obtained ferroptosis-related genes (FRGs) in FerrDb database and clinical transcriptome data in TCGA database and GEO database. Consensus cluster analysis was used to identify three molecular markers of ferroptosis in PCa with differential expression of 40 FRGs, including PD-L1 expression levels. We conducted a new ferroptosis-related signature for PCa RFS using four FRGs identified through univariate and multivariate Cox regression analyses. The signature was validated in the training, testing, and validation cohorts, and it demonstrated remarkable results in the area under the time-dependent receiver operating characteristic (ROC) curve of 0.757, 0.715, and 0.732, respectively. Additionally, we observed that younger patients, those with stage T III and stage T IV, stage N0, cluster 1, and cluster 2 PCa were more accurately predicted by the signature as independent predictors of RFS. DU-145 and RWPE-1 cells were successfully analyzed by qRT-PCR and Western blot for ASNS, GPT2, RRM2, and NFE2L2. In summary, we developed a novel ferroptosis-based signature for RFS in PC, utilizing four FRGs identified through univariate and multivariate Cox regression analyses. This signature was rigorously validated across training, testing, and validation cohorts, demonstrating exceptional performance as evidenced by its ROC curves. Notably, our findings indicate that this signature is particularly effective as an independent predictor of RFS in younger patients or those with stage T III and T IV, stage N0, and in clusters 1 and 2. Finally, we confirmed the expression of these four FRGs in DU-145 and RWPE-1 cell lines.https://doi.org/10.1038/s41598-023-50205-1 |
| spellingShingle | Jinquan Chen Longbin Zhang Yiling Luo Chao Tan Huang Hu Yuling Jiang Na Xi Qinghai Zeng H. Peng Development of a ferroptosis-based molecular markers for predicting RFS in prostate cancer patients Scientific Reports |
| title | Development of a ferroptosis-based molecular markers for predicting RFS in prostate cancer patients |
| title_full | Development of a ferroptosis-based molecular markers for predicting RFS in prostate cancer patients |
| title_fullStr | Development of a ferroptosis-based molecular markers for predicting RFS in prostate cancer patients |
| title_full_unstemmed | Development of a ferroptosis-based molecular markers for predicting RFS in prostate cancer patients |
| title_short | Development of a ferroptosis-based molecular markers for predicting RFS in prostate cancer patients |
| title_sort | development of a ferroptosis based molecular markers for predicting rfs in prostate cancer patients |
| url | https://doi.org/10.1038/s41598-023-50205-1 |
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