Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS
Beans, a globally significant economic and nutritional food crop, are rich in polyphenolic chemicals with potential health advantages, providing high protein, fiber, minerals, and vitamins. However, studies on the global profiling of lipids in beans are limited. We applied a non-targeted lipidomic a...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/14/2784 |
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| author | Rachana M. Gangadhara Siddabasave Gowda B. Gowda Divyavani Gowda Ken Inui Shu-Ping Hui |
| author_facet | Rachana M. Gangadhara Siddabasave Gowda B. Gowda Divyavani Gowda Ken Inui Shu-Ping Hui |
| author_sort | Rachana M. Gangadhara |
| collection | DOAJ |
| description | Beans, a globally significant economic and nutritional food crop, are rich in polyphenolic chemicals with potential health advantages, providing high protein, fiber, minerals, and vitamins. However, studies on the global profiling of lipids in beans are limited. We applied a non-targeted lipidomic approach based on high-performance liquid chromatography coupled with linear ion trap–Orbitrap mass spectrometry (HPLC/LTQ-Orbitrap-MS) to comprehensively profile and compare the lipids in six distinct bean cultivars, namely, adzuki red beans—adzuki cultivar (ARB-AC), adzuki red beans—Benidainagon cultivar (ARB-BC), adzuki red beans—Erimoshouzu cultivar (ARB-EC), soybean—Fukuyutaka cultivar 2021 (SB-FC21), soybean—Fukuyutaka cultivar 2022 (SB-FC22), and soybean—Oosuzu cultivar (SB-OC). MS/MS analysis defined 144 molecular species from four main lipid groups. Multivariate principal component analysis indicated unique lipid compositions in the cultivars except for ARB-BC and ARB-EC. Evaluation of the concentrations of polyunsaturated fatty acid to saturated fatty acid ratio among all the cultivars showed that SB-FC21 and SB-FC22 had the highest value, suggesting they are the most beneficial for health. Furthermore, lipids such as acyl sterol glycosides were detected and characterized for the first time in these bean cultivars. Hierarchical cluster correlations revealed the predominance of ceramides in ARB-EC, lysophospholipids in SB-FC21, and glycerophospholipids in SB-OC. This study comprehensively investigated lipids and their compositions in beans, indicating their potential utility in the nutritional evaluation of beans as functional foods. |
| first_indexed | 2024-03-11T01:04:20Z |
| format | Article |
| id | doaj.art-2015c1edf7084c5fa4339ced0482a4bf |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T01:04:20Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-2015c1edf7084c5fa4339ced0482a4bf2023-11-18T19:21:26ZengMDPI AGFoods2304-81582023-07-011214278410.3390/foods12142784Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MSRachana M. Gangadhara0Siddabasave Gowda B. Gowda1Divyavani Gowda2Ken Inui3Shu-Ping Hui4Graduate School of Global Food Resources, Hokkaido University, Kita-9, Nishi-9, Kita-Ku, Sapporo 060-0809, JapanGraduate School of Global Food Resources, Hokkaido University, Kita-9, Nishi-9, Kita-Ku, Sapporo 060-0809, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanHIRYU Co., Ltd., Chuo-Cho 2-32, Kashiwa-shi 277-0021, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanBeans, a globally significant economic and nutritional food crop, are rich in polyphenolic chemicals with potential health advantages, providing high protein, fiber, minerals, and vitamins. However, studies on the global profiling of lipids in beans are limited. We applied a non-targeted lipidomic approach based on high-performance liquid chromatography coupled with linear ion trap–Orbitrap mass spectrometry (HPLC/LTQ-Orbitrap-MS) to comprehensively profile and compare the lipids in six distinct bean cultivars, namely, adzuki red beans—adzuki cultivar (ARB-AC), adzuki red beans—Benidainagon cultivar (ARB-BC), adzuki red beans—Erimoshouzu cultivar (ARB-EC), soybean—Fukuyutaka cultivar 2021 (SB-FC21), soybean—Fukuyutaka cultivar 2022 (SB-FC22), and soybean—Oosuzu cultivar (SB-OC). MS/MS analysis defined 144 molecular species from four main lipid groups. Multivariate principal component analysis indicated unique lipid compositions in the cultivars except for ARB-BC and ARB-EC. Evaluation of the concentrations of polyunsaturated fatty acid to saturated fatty acid ratio among all the cultivars showed that SB-FC21 and SB-FC22 had the highest value, suggesting they are the most beneficial for health. Furthermore, lipids such as acyl sterol glycosides were detected and characterized for the first time in these bean cultivars. Hierarchical cluster correlations revealed the predominance of ceramides in ARB-EC, lysophospholipids in SB-FC21, and glycerophospholipids in SB-OC. This study comprehensively investigated lipids and their compositions in beans, indicating their potential utility in the nutritional evaluation of beans as functional foods.https://www.mdpi.com/2304-8158/12/14/2784liquid chromatographymass spectrometrybean sampleslipid analysiscluster correlation |
| spellingShingle | Rachana M. Gangadhara Siddabasave Gowda B. Gowda Divyavani Gowda Ken Inui Shu-Ping Hui Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS Foods liquid chromatography mass spectrometry bean samples lipid analysis cluster correlation |
| title | Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS |
| title_full | Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS |
| title_fullStr | Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS |
| title_full_unstemmed | Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS |
| title_short | Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS |
| title_sort | lipid composition analysis and characterization of acyl sterol glycosides in adzuki and soybean cultivars by non targeted lc ms |
| topic | liquid chromatography mass spectrometry bean samples lipid analysis cluster correlation |
| url | https://www.mdpi.com/2304-8158/12/14/2784 |
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