Virome Analysis of <i>Aconitum carmichaelii</i> Reveals Infection by Eleven Viruses, including Two Potentially New Species
<i>Aconitum carmichaelii</i> is a herbaceous herb indigenous to China that has been cultivated for traditional medicine for centuries. Virus-like symptoms of <i>A. carmichaelii</i> plants were observed on leaves in some <i>A. carmichaelii</i> plantations in Zhanyi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/21/15558 |
| Summary: | <i>Aconitum carmichaelii</i> is a herbaceous herb indigenous to China that has been cultivated for traditional medicine for centuries. Virus-like symptoms of <i>A. carmichaelii</i> plants were observed on leaves in some <i>A. carmichaelii</i> plantations in Zhanyi and Wuding Counties, Yunnan Province, southwest China. High-throughput sequencing (HTS) was performed on 28 symptomatic plants, and the results revealed infection with 11 viruses, including 2 novel viruses and 9 previously described viruses: Aconitum amalgavirus 1 (AcoAV-1), aconite virus A (AcVA), cucumber mosaic virus (CMV), currant latent virus (CuLV), apple stem grooving virus (ASGV), chilli veinal mottle virus (ChiVMV), tomato spotted wilt orthotospovirus (TSWV), tobacco vein distorting virus (TVDV), and potato leafroll virus (PLRV). Two novel viruses tentatively named Aconitum potyvirus 1 and Aconitum betapartitivirus 1, were supported by sequence and phylogenetic analysis results of their genomes. We proposed the names <i>Potyvirus aconiti</i> and <i>Betapartitivirus aconiti</i>. RT-PCR assays of 142 plants revealed the predominance and widespread distribution of CMV, AcVA, and AcoPV-1 in plantations. The detection of isolates of CuLV, ASGV, ChiVMV, TSWV, TVDV, and PLRV infections for the first time in <i>A. carmichaelii</i> expands their known host ranges. |
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| ISSN: | 1661-6596 1422-0067 |