Virome Analysis of <i>Aconitum carmichaelii</i> Reveals Infection by Eleven Viruses, including Two Potentially New Species
<i>Aconitum carmichaelii</i> is a herbaceous herb indigenous to China that has been cultivated for traditional medicine for centuries. Virus-like symptoms of <i>A. carmichaelii</i> plants were observed on leaves in some <i>A. carmichaelii</i> plantations in Zhanyi...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/21/15558 |
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| author | Jie Yang Ping-Xiu Lan Yun Wang Jin-Ming Li Ruhui Li Steve Wylie Xiao-Jiao Chen Gen-Hua Yang Hong Cai Fan Li |
| author_facet | Jie Yang Ping-Xiu Lan Yun Wang Jin-Ming Li Ruhui Li Steve Wylie Xiao-Jiao Chen Gen-Hua Yang Hong Cai Fan Li |
| author_sort | Jie Yang |
| collection | DOAJ |
| description | <i>Aconitum carmichaelii</i> is a herbaceous herb indigenous to China that has been cultivated for traditional medicine for centuries. Virus-like symptoms of <i>A. carmichaelii</i> plants were observed on leaves in some <i>A. carmichaelii</i> plantations in Zhanyi and Wuding Counties, Yunnan Province, southwest China. High-throughput sequencing (HTS) was performed on 28 symptomatic plants, and the results revealed infection with 11 viruses, including 2 novel viruses and 9 previously described viruses: Aconitum amalgavirus 1 (AcoAV-1), aconite virus A (AcVA), cucumber mosaic virus (CMV), currant latent virus (CuLV), apple stem grooving virus (ASGV), chilli veinal mottle virus (ChiVMV), tomato spotted wilt orthotospovirus (TSWV), tobacco vein distorting virus (TVDV), and potato leafroll virus (PLRV). Two novel viruses tentatively named Aconitum potyvirus 1 and Aconitum betapartitivirus 1, were supported by sequence and phylogenetic analysis results of their genomes. We proposed the names <i>Potyvirus aconiti</i> and <i>Betapartitivirus aconiti</i>. RT-PCR assays of 142 plants revealed the predominance and widespread distribution of CMV, AcVA, and AcoPV-1 in plantations. The detection of isolates of CuLV, ASGV, ChiVMV, TSWV, TVDV, and PLRV infections for the first time in <i>A. carmichaelii</i> expands their known host ranges. |
| first_indexed | 2024-03-11T11:29:25Z |
| format | Article |
| id | doaj.art-201784a7f36c4d19ac8c12b107217f57 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T11:29:25Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-201784a7f36c4d19ac8c12b107217f572023-11-10T15:04:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124211555810.3390/ijms242115558Virome Analysis of <i>Aconitum carmichaelii</i> Reveals Infection by Eleven Viruses, including Two Potentially New SpeciesJie Yang0Ping-Xiu Lan1Yun Wang2Jin-Ming Li3Ruhui Li4Steve Wylie5Xiao-Jiao Chen6Gen-Hua Yang7Hong Cai8Fan Li9State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaUSDA-ARS, National Germplasm Resources Laboratory, Beltsville, MD 20705, USAPlant Biotechnology Research Group (Virology), Western Australian State Agricultural Biotechnology Centre, Murdoch University, Murdoch, WA 6150, AustraliaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China<i>Aconitum carmichaelii</i> is a herbaceous herb indigenous to China that has been cultivated for traditional medicine for centuries. Virus-like symptoms of <i>A. carmichaelii</i> plants were observed on leaves in some <i>A. carmichaelii</i> plantations in Zhanyi and Wuding Counties, Yunnan Province, southwest China. High-throughput sequencing (HTS) was performed on 28 symptomatic plants, and the results revealed infection with 11 viruses, including 2 novel viruses and 9 previously described viruses: Aconitum amalgavirus 1 (AcoAV-1), aconite virus A (AcVA), cucumber mosaic virus (CMV), currant latent virus (CuLV), apple stem grooving virus (ASGV), chilli veinal mottle virus (ChiVMV), tomato spotted wilt orthotospovirus (TSWV), tobacco vein distorting virus (TVDV), and potato leafroll virus (PLRV). Two novel viruses tentatively named Aconitum potyvirus 1 and Aconitum betapartitivirus 1, were supported by sequence and phylogenetic analysis results of their genomes. We proposed the names <i>Potyvirus aconiti</i> and <i>Betapartitivirus aconiti</i>. RT-PCR assays of 142 plants revealed the predominance and widespread distribution of CMV, AcVA, and AcoPV-1 in plantations. The detection of isolates of CuLV, ASGV, ChiVMV, TSWV, TVDV, and PLRV infections for the first time in <i>A. carmichaelii</i> expands their known host ranges.https://www.mdpi.com/1422-0067/24/21/15558traditional Chinese medicinevirus discoveryviromehigh-throughput sequencing<i>Potyvirus aconiti</i><i>Betapartitivirus aconiti</i> |
| spellingShingle | Jie Yang Ping-Xiu Lan Yun Wang Jin-Ming Li Ruhui Li Steve Wylie Xiao-Jiao Chen Gen-Hua Yang Hong Cai Fan Li Virome Analysis of <i>Aconitum carmichaelii</i> Reveals Infection by Eleven Viruses, including Two Potentially New Species International Journal of Molecular Sciences traditional Chinese medicine virus discovery virome high-throughput sequencing <i>Potyvirus aconiti</i> <i>Betapartitivirus aconiti</i> |
| title | Virome Analysis of <i>Aconitum carmichaelii</i> Reveals Infection by Eleven Viruses, including Two Potentially New Species |
| title_full | Virome Analysis of <i>Aconitum carmichaelii</i> Reveals Infection by Eleven Viruses, including Two Potentially New Species |
| title_fullStr | Virome Analysis of <i>Aconitum carmichaelii</i> Reveals Infection by Eleven Viruses, including Two Potentially New Species |
| title_full_unstemmed | Virome Analysis of <i>Aconitum carmichaelii</i> Reveals Infection by Eleven Viruses, including Two Potentially New Species |
| title_short | Virome Analysis of <i>Aconitum carmichaelii</i> Reveals Infection by Eleven Viruses, including Two Potentially New Species |
| title_sort | virome analysis of i aconitum carmichaelii i reveals infection by eleven viruses including two potentially new species |
| topic | traditional Chinese medicine virus discovery virome high-throughput sequencing <i>Potyvirus aconiti</i> <i>Betapartitivirus aconiti</i> |
| url | https://www.mdpi.com/1422-0067/24/21/15558 |
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