(GTG)5-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso
Abstract Background Escherichia coli (E. coli) is the most common bacterial species implicated in various types of infections including septicemia, gastroenteritis, urinary tract infections, meningitis and others pathologies. These involve several bacterial clones with multidrug resistance making th...
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-022-02537-7 |
| _version_ | 1811289664424771584 |
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| author | Boukaré Kaboré Ganamé Abasse Ouédraogo Hama Cissé Henri S. Ouédraogo Emmanuel Sampo Koudbi Jacob Zongo Boukaré Zeba Yves Traoré Olivier Gnankiné Idrissa Sanou Aly Savadogo |
| author_facet | Boukaré Kaboré Ganamé Abasse Ouédraogo Hama Cissé Henri S. Ouédraogo Emmanuel Sampo Koudbi Jacob Zongo Boukaré Zeba Yves Traoré Olivier Gnankiné Idrissa Sanou Aly Savadogo |
| author_sort | Boukaré Kaboré |
| collection | DOAJ |
| description | Abstract Background Escherichia coli (E. coli) is the most common bacterial species implicated in various types of infections including septicemia, gastroenteritis, urinary tract infections, meningitis and others pathologies. These involve several bacterial clones with multidrug resistance making them difficult to treat. The aims of this study was to perform molecular typing of E. coli strains using universal primer (GTG)5. In this study, 53 E. coli strains were collected from inpatients and outpatients. The test of antimicrobial sensibility was realized using CA-SFM /EUCAST method and strains were identified by conventional microbiological tests. The carbapenemase-producing strains were demonstrated by phenotypic method. Bacterial DNA was extracted by boiling method. (GTG)5-PCR was used for strain subtyping. The DendroUPGMA software was used for grouping of strains from the genetic fingerprints obtained by (GTG)5-PCR. Results Antibiotic susceptibility test revealed that all strains were multi-drug resistant (MDR). Its strains showed resistance to at least three different families of antibiotics. Of this MDR strains, only one was a metallo-β-lactamase producer. The dendrogram obtained using genetic fingerprinting allowed the E. coli strains to be grouped into 22 clusters (G1 to G22). Conclusion The (GTG) 5-PCR assay enabled rapid molecular typing of E. coli strains. The strains of E. coli typed in this study would belong to different clones. |
| first_indexed | 2024-04-13T03:58:27Z |
| format | Article |
| id | doaj.art-2018163e6456469196f2bb76dd3ed673 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-13T03:58:27Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-2018163e6456469196f2bb76dd3ed6732022-12-22T03:03:32ZengBMCBMC Microbiology1471-21802022-04-012211810.1186/s12866-022-02537-7(GTG)5-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina FasoBoukaré Kaboré0Ganamé Abasse Ouédraogo1Hama Cissé2Henri S. Ouédraogo3Emmanuel Sampo4Koudbi Jacob Zongo5Boukaré Zeba6Yves Traoré7Olivier Gnankiné8Idrissa Sanou9Aly Savadogo10Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBODepartment of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBODepartment of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBODepartment of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBOSPS of SchiphraDepartment of Biochemistry and Microbiology, Faculty of Applied Science and Technology, University of DedougouDepartment of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBODepartment of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBODepartment of Biology and Physiology Aminale, University Joseph KI-ZERBOUFR Health Sciences, University Joseph KI-ZERBODepartment of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBOAbstract Background Escherichia coli (E. coli) is the most common bacterial species implicated in various types of infections including septicemia, gastroenteritis, urinary tract infections, meningitis and others pathologies. These involve several bacterial clones with multidrug resistance making them difficult to treat. The aims of this study was to perform molecular typing of E. coli strains using universal primer (GTG)5. In this study, 53 E. coli strains were collected from inpatients and outpatients. The test of antimicrobial sensibility was realized using CA-SFM /EUCAST method and strains were identified by conventional microbiological tests. The carbapenemase-producing strains were demonstrated by phenotypic method. Bacterial DNA was extracted by boiling method. (GTG)5-PCR was used for strain subtyping. The DendroUPGMA software was used for grouping of strains from the genetic fingerprints obtained by (GTG)5-PCR. Results Antibiotic susceptibility test revealed that all strains were multi-drug resistant (MDR). Its strains showed resistance to at least three different families of antibiotics. Of this MDR strains, only one was a metallo-β-lactamase producer. The dendrogram obtained using genetic fingerprinting allowed the E. coli strains to be grouped into 22 clusters (G1 to G22). Conclusion The (GTG) 5-PCR assay enabled rapid molecular typing of E. coli strains. The strains of E. coli typed in this study would belong to different clones.https://doi.org/10.1186/s12866-022-02537-7E. coliMultidrug resistance(GTG)5-PCRMolecular typing |
| spellingShingle | Boukaré Kaboré Ganamé Abasse Ouédraogo Hama Cissé Henri S. Ouédraogo Emmanuel Sampo Koudbi Jacob Zongo Boukaré Zeba Yves Traoré Olivier Gnankiné Idrissa Sanou Aly Savadogo (GTG)5-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso BMC Microbiology E. coli Multidrug resistance (GTG)5-PCR Molecular typing |
| title | (GTG)5-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso |
| title_full | (GTG)5-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso |
| title_fullStr | (GTG)5-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso |
| title_full_unstemmed | (GTG)5-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso |
| title_short | (GTG)5-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso |
| title_sort | gtg 5 pcr fingerprinting of multi drug resistant escherichia coli bacteria isolates from hospital in ouagadougou burkina faso |
| topic | E. coli Multidrug resistance (GTG)5-PCR Molecular typing |
| url | https://doi.org/10.1186/s12866-022-02537-7 |
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