Serological reactivity of inactivated SARS-CoV-2 vaccine based on an S-RBD neutralizing antibody assay
Objectives: This study aimed to explore the regularity of S-RBD domain antibody reactivity after immunization with inactivated SARS-CoV-2 vaccine and evaluate the effect of this vaccine on the immune response. Design or methods: Venous blood samples were collected from 1156 healthcare workers who pa...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971222000698 |
| _version_ | 1830211457389690880 |
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| author | Hui Zhang Xingtian Liu Qingzhi Liu Hongshuang Mei Yashu Wang Gang Cui Shuping Zhao |
| author_facet | Hui Zhang Xingtian Liu Qingzhi Liu Hongshuang Mei Yashu Wang Gang Cui Shuping Zhao |
| author_sort | Hui Zhang |
| collection | DOAJ |
| description | Objectives: This study aimed to explore the regularity of S-RBD domain antibody reactivity after immunization with inactivated SARS-CoV-2 vaccine and evaluate the effect of this vaccine on the immune response. Design or methods: Venous blood samples were collected from 1156 healthcare workers who participated in the phase III clinical trial of the SARS-CoV-2 inactivated vaccine. The S-RBD domain antibody levels in the serum were detected by ELISA 14 days after the first and second active immunization, respectively. Results: The positive rates after inoculation of the first and second vaccination of S-RBD domain antibody against SARS-CoV-2 were 28.03% and 86.76%, respectively. The mean inhibition rate of S-RBD domain antibody against positive samples was 57.18 ± 18.87% after the second vaccination at 14 days. Sex and age had no effects on the positive rate. The positive rate was decreased in the high BMI group. Single-factor logistic analysis showed that there was no significant correlation between age and positive rate. BMI was negatively correlated with the positive rate. Conclusions: After 2 immunizations, the positive rate of SARS-CoV-2 S-RBD domain antibody was high, and the vaccine had good immunogenicity. The improvement of the immune strategy should focus on the effects of BMI and other factors. |
| first_indexed | 2024-12-18T05:44:43Z |
| format | Article |
| id | doaj.art-201970650ded48f09e838dcd857c0cb9 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-18T05:44:43Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-201970650ded48f09e838dcd857c0cb92022-12-21T21:19:05ZengElsevierInternational Journal of Infectious Diseases1201-97122022-04-01117169173Serological reactivity of inactivated SARS-CoV-2 vaccine based on an S-RBD neutralizing antibody assayHui Zhang0Xingtian Liu1Qingzhi Liu2Hongshuang Mei3Yashu Wang4Gang Cui5Shuping Zhao6Department of Clinic Lab Center, Taian City Central Hospital, Taian, 271000, ChinaDepartment of Clinic Lab Center, Taian City Central Hospital, Taian, 271000, ChinaDepartment of Clinic Lab Center, Taian City Central Hospital, Taian, 271000, ChinaDepartment of Clinic Lab Center, Taian City Central Hospital, Taian, 271000, ChinaDepartment of Clinic Lab Center, Taian City Central Hospital, Taian, 271000, ChinaDepartment of Clinic Lab Center, Taian City Central Hospital, Taian, 271000, ChinaCorresponding Author: Shuping Zhao, Department of Clinic Lab Center, Taian City Central Hospital, 29 Longtan Road, Taian, 271000, Shandong, China.; Department of Clinic Lab Center, Taian City Central Hospital, Taian, 271000, ChinaObjectives: This study aimed to explore the regularity of S-RBD domain antibody reactivity after immunization with inactivated SARS-CoV-2 vaccine and evaluate the effect of this vaccine on the immune response. Design or methods: Venous blood samples were collected from 1156 healthcare workers who participated in the phase III clinical trial of the SARS-CoV-2 inactivated vaccine. The S-RBD domain antibody levels in the serum were detected by ELISA 14 days after the first and second active immunization, respectively. Results: The positive rates after inoculation of the first and second vaccination of S-RBD domain antibody against SARS-CoV-2 were 28.03% and 86.76%, respectively. The mean inhibition rate of S-RBD domain antibody against positive samples was 57.18 ± 18.87% after the second vaccination at 14 days. Sex and age had no effects on the positive rate. The positive rate was decreased in the high BMI group. Single-factor logistic analysis showed that there was no significant correlation between age and positive rate. BMI was negatively correlated with the positive rate. Conclusions: After 2 immunizations, the positive rate of SARS-CoV-2 S-RBD domain antibody was high, and the vaccine had good immunogenicity. The improvement of the immune strategy should focus on the effects of BMI and other factors.http://www.sciencedirect.com/science/article/pii/S1201971222000698COVID-19Inactivated SARS-CoV-2 vaccineS-RBD domain antibodyImmunogenicityBMI |
| spellingShingle | Hui Zhang Xingtian Liu Qingzhi Liu Hongshuang Mei Yashu Wang Gang Cui Shuping Zhao Serological reactivity of inactivated SARS-CoV-2 vaccine based on an S-RBD neutralizing antibody assay International Journal of Infectious Diseases COVID-19 Inactivated SARS-CoV-2 vaccine S-RBD domain antibody Immunogenicity BMI |
| title | Serological reactivity of inactivated SARS-CoV-2 vaccine based on an S-RBD neutralizing antibody assay |
| title_full | Serological reactivity of inactivated SARS-CoV-2 vaccine based on an S-RBD neutralizing antibody assay |
| title_fullStr | Serological reactivity of inactivated SARS-CoV-2 vaccine based on an S-RBD neutralizing antibody assay |
| title_full_unstemmed | Serological reactivity of inactivated SARS-CoV-2 vaccine based on an S-RBD neutralizing antibody assay |
| title_short | Serological reactivity of inactivated SARS-CoV-2 vaccine based on an S-RBD neutralizing antibody assay |
| title_sort | serological reactivity of inactivated sars cov 2 vaccine based on an s rbd neutralizing antibody assay |
| topic | COVID-19 Inactivated SARS-CoV-2 vaccine S-RBD domain antibody Immunogenicity BMI |
| url | http://www.sciencedirect.com/science/article/pii/S1201971222000698 |
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