Novel bio compactable silver nanowires and nanocubes: An effective treatment against carbapenem and vancomycin resistant strains isolated from cancer patients
Blood stream infections in the cancer patients are a critical problem which leads to considerable rate of mortality and morbidity. In view of this herein, we account the response of Poly Vinyl Pyrrolidone (PVP) coated silver nanowires (Ag-NWs) and silver nano cubes (Ag-NCs) towards carbapenem (Esche...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-12-01
|
| Series: | Journal of Saudi Chemical Society |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319610319300717 |
| _version_ | 1819035133665083392 |
|---|---|
| author | Jiya Jose Anju K. Nair Nandakumar Kalarikkal Oluwatobi Oluwafemi Sabu Thomas |
| author_facet | Jiya Jose Anju K. Nair Nandakumar Kalarikkal Oluwatobi Oluwafemi Sabu Thomas |
| author_sort | Jiya Jose |
| collection | DOAJ |
| description | Blood stream infections in the cancer patients are a critical problem which leads to considerable rate of mortality and morbidity. In view of this herein, we account the response of Poly Vinyl Pyrrolidone (PVP) coated silver nanowires (Ag-NWs) and silver nano cubes (Ag-NCs) towards carbapenem (Escherichia coli) and vancomycin (Staphylococcus aureus) resistant strains as well as to human epithelial cells. The prepared PVP capped silver nanomaterials were in the range of 80 nm (Ag-NC) and 25 nm (Ag-NW) as observed from Transmission Electron Microscopy (TEM). Though the selected strains were resistant to carbapenem and vancomycin, PVP capped Ag-NWs and Ag-NCs could inhibit the growth of these strains. These nanomaterials could break the cell wall and damage the genetic material of these strains as observed by death rate assay and alkaline comet assay. Furthermore, we have shown that toxicity of Ag-NWs and Ag-NCs precisely follows the dose retort pattern. Even though the resistant strains were susceptible to a concentration of 10 µg ml−1 of silver nanomaterials the epithelial cells were not affected by the same concentration. It is also confirmed with live dead staining assay and observed that metabolic activities of epithelial cells were not affected by a concentration of 10 µg ml−1. Overall, this work suggests that these nanomaterials can be utilized to treat the multiple drug resistant strains from cancer patients. Keywords: Silver nanomaterials, Multiple drug resistance, Septicaemia, Genotoxicity, Biocompatibility, Cancer |
| first_indexed | 2024-12-21T07:44:47Z |
| format | Article |
| id | doaj.art-20197ee9fa2e4b76b0f8aa125571aade |
| institution | Directory Open Access Journal |
| issn | 1319-6103 |
| language | English |
| last_indexed | 2024-12-21T07:44:47Z |
| publishDate | 2019-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Saudi Chemical Society |
| spelling | doaj.art-20197ee9fa2e4b76b0f8aa125571aade2022-12-21T19:11:14ZengElsevierJournal of Saudi Chemical Society1319-61032019-12-0123810901101Novel bio compactable silver nanowires and nanocubes: An effective treatment against carbapenem and vancomycin resistant strains isolated from cancer patientsJiya Jose0Anju K. Nair1Nandakumar Kalarikkal2Oluwatobi Oluwafemi3Sabu Thomas4International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala 686560, IndiaInternational and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala 686560, India; Department of Physics, St. Teresa’s College, Ernakulam, Kerala, IndiaInternational and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala 686560, India; School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, Kerala 686560, IndiaDepartment of Applied Chemistry, University of Johannesburg, P. O. Box 17011, Doornfontein 2028, Johannesburg, South Africa; Centre for Nanomaterials Science Research, University of Johannesburg, Johannesburg, South AfricaInternational and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala 686560, India; School of Chemical Sciences, Mahatma Gandhi University, Kottayam, Kerala 686560, India; Corresponding author.Blood stream infections in the cancer patients are a critical problem which leads to considerable rate of mortality and morbidity. In view of this herein, we account the response of Poly Vinyl Pyrrolidone (PVP) coated silver nanowires (Ag-NWs) and silver nano cubes (Ag-NCs) towards carbapenem (Escherichia coli) and vancomycin (Staphylococcus aureus) resistant strains as well as to human epithelial cells. The prepared PVP capped silver nanomaterials were in the range of 80 nm (Ag-NC) and 25 nm (Ag-NW) as observed from Transmission Electron Microscopy (TEM). Though the selected strains were resistant to carbapenem and vancomycin, PVP capped Ag-NWs and Ag-NCs could inhibit the growth of these strains. These nanomaterials could break the cell wall and damage the genetic material of these strains as observed by death rate assay and alkaline comet assay. Furthermore, we have shown that toxicity of Ag-NWs and Ag-NCs precisely follows the dose retort pattern. Even though the resistant strains were susceptible to a concentration of 10 µg ml−1 of silver nanomaterials the epithelial cells were not affected by the same concentration. It is also confirmed with live dead staining assay and observed that metabolic activities of epithelial cells were not affected by a concentration of 10 µg ml−1. Overall, this work suggests that these nanomaterials can be utilized to treat the multiple drug resistant strains from cancer patients. Keywords: Silver nanomaterials, Multiple drug resistance, Septicaemia, Genotoxicity, Biocompatibility, Cancerhttp://www.sciencedirect.com/science/article/pii/S1319610319300717 |
| spellingShingle | Jiya Jose Anju K. Nair Nandakumar Kalarikkal Oluwatobi Oluwafemi Sabu Thomas Novel bio compactable silver nanowires and nanocubes: An effective treatment against carbapenem and vancomycin resistant strains isolated from cancer patients Journal of Saudi Chemical Society |
| title | Novel bio compactable silver nanowires and nanocubes: An effective treatment against carbapenem and vancomycin resistant strains isolated from cancer patients |
| title_full | Novel bio compactable silver nanowires and nanocubes: An effective treatment against carbapenem and vancomycin resistant strains isolated from cancer patients |
| title_fullStr | Novel bio compactable silver nanowires and nanocubes: An effective treatment against carbapenem and vancomycin resistant strains isolated from cancer patients |
| title_full_unstemmed | Novel bio compactable silver nanowires and nanocubes: An effective treatment against carbapenem and vancomycin resistant strains isolated from cancer patients |
| title_short | Novel bio compactable silver nanowires and nanocubes: An effective treatment against carbapenem and vancomycin resistant strains isolated from cancer patients |
| title_sort | novel bio compactable silver nanowires and nanocubes an effective treatment against carbapenem and vancomycin resistant strains isolated from cancer patients |
| url | http://www.sciencedirect.com/science/article/pii/S1319610319300717 |
| work_keys_str_mv | AT jiyajose novelbiocompactablesilvernanowiresandnanocubesaneffectivetreatmentagainstcarbapenemandvancomycinresistantstrainsisolatedfromcancerpatients AT anjuknair novelbiocompactablesilvernanowiresandnanocubesaneffectivetreatmentagainstcarbapenemandvancomycinresistantstrainsisolatedfromcancerpatients AT nandakumarkalarikkal novelbiocompactablesilvernanowiresandnanocubesaneffectivetreatmentagainstcarbapenemandvancomycinresistantstrainsisolatedfromcancerpatients AT oluwatobioluwafemi novelbiocompactablesilvernanowiresandnanocubesaneffectivetreatmentagainstcarbapenemandvancomycinresistantstrainsisolatedfromcancerpatients AT sabuthomas novelbiocompactablesilvernanowiresandnanocubesaneffectivetreatmentagainstcarbapenemandvancomycinresistantstrainsisolatedfromcancerpatients |