Using a zebrafish model to understand adherent-invasive Escherichia coli infection

Using a zebrafish model to understand adherent-invasive Escherichia coli infection

Summary: A zebrafish model was developed to study AIEC colonization, invasion, and inflammation. This model can also be used to study the beneficial effects of a probiotic on AIEC infection of adult zebrafish. Bacteria are grown in vitro and then fish are infected with AIEC by immersion. Subsequentl...

Full description

Bibliographic Details
Main Authors: Dhrubajyoti Nag, Dustin Farr, Saumya Raychaudhuri, Jeffrey H. Withey
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Immunology
Microbiology
Model organisms
Molecular biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006906
Description
Summary:Summary: A zebrafish model was developed to study AIEC colonization, invasion, and inflammation. This model can also be used to study the beneficial effects of a probiotic on AIEC infection of adult zebrafish. Bacteria are grown in vitro and then fish are infected with AIEC by immersion. Subsequently, colonization and inflammation can be assessed. Exposing fish to probiotic at different time points relative to AIEC can determine beneficial effects of probiotics as prophylactics or therapeutics against AIEC.For complete details on the use and execution of this protocol, please refer to Nag et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

Similar Items