Bioactive engineered scaffolds based on PCL-PEG-PCL and tumor cell-derived exosomes to minimize the foreign body reaction
Long-term presence of M1 macrophages causes serious foreign body reaction (FBR), which is the main reason for the failure of biological scaffold integration. Inducing M2 polarization of macrophages near scaffolds to reduce foreign body response has been widely researched. In this work, inspired by t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-08-01
|
| Series: | Biomaterials and Biosystems |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666534422000174 |
| _version_ | 1828422660121427968 |
|---|---|
| author | Zehong Xiang Xinghua Guan Zhifang Ma Qiang Shi Mikhail Panteleev Fazly I. Ataullakhanov |
| author_facet | Zehong Xiang Xinghua Guan Zhifang Ma Qiang Shi Mikhail Panteleev Fazly I. Ataullakhanov |
| author_sort | Zehong Xiang |
| collection | DOAJ |
| description | Long-term presence of M1 macrophages causes serious foreign body reaction (FBR), which is the main reason for the failure of biological scaffold integration. Inducing M2 polarization of macrophages near scaffolds to reduce foreign body response has been widely researched. In this work, inspired by the special capability of tumor exosomes in macrophages M2 polarization, we integrate tumor-derived exosomes into biological scaffolds to minimize the FBR. In brief, breast cancer cell-derived exosomes are loaded into polycaprolactone-b-polyethylene glycol-b-polycaprolactone (PCL-PEG-PCL) fiber scaffold through physical adsorption and entrapment to constructed bioactive engineered scaffold. In cellular experiments, we demonstrate bioactive engineered scaffold based on PCL-PEG-PCL and exosomes can promote the transformation of macrophages from M1 to M2 through the PI3K/Akt signaling pathway. In addition, the exosomes release gradually from scaffolds and act on the macrophages around the scaffolds to reduce FBR in a subcutaneous implant mouse model. Compared with PCL-PEG-PCL scaffolds without exosomes, bioactive engineered scaffolds reduce significantly inflammation and fibrosis of tissues around the scaffolds. Therefore, cancer cell-derived exosomes show the potential for constructing engineered scaffolds in inhibiting the excessive inflammation and facilitating tissue formation. |
| first_indexed | 2024-12-10T15:51:22Z |
| format | Article |
| id | doaj.art-201d82f2ba7643f2853adcf4bebcb746 |
| institution | Directory Open Access Journal |
| issn | 2666-5344 |
| language | English |
| last_indexed | 2024-12-10T15:51:22Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomaterials and Biosystems |
| spelling | doaj.art-201d82f2ba7643f2853adcf4bebcb7462022-12-22T01:42:48ZengElsevierBiomaterials and Biosystems2666-53442022-08-017100055Bioactive engineered scaffolds based on PCL-PEG-PCL and tumor cell-derived exosomes to minimize the foreign body reactionZehong Xiang0Xinghua Guan1Zhifang Ma2Qiang Shi3Mikhail Panteleev4Fazly I. Ataullakhanov5State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; University of Science and Technology of China, Hefei, Anhui 230026, ChinaState Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; University of Science and Technology of China, Hefei, Anhui 230026, ChinaState Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, ChinaState Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; University of Science and Technology of China, Hefei, Anhui 230026, China; Key Laboratory of Polymeric Materials Design and Synthesis for Biomedical Function, Soochow University, Suzhou 215123, China; Corresponding author at: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.Dmitry Rogachev Natl Res Ctr Pediat Hematol Oncol, 1 Samory Mashela St, Moscow, 117198, Russia; Faculty of Physics, Lomonosov Moscow State University, Leninskie Gory, 1, build. 2, GSP-1, Moscow 119991, RussiaDmitry Rogachev Natl Res Ctr Pediat Hematol Oncol, 1 Samory Mashela St, Moscow, 117198, Russia; Faculty of Physics, Lomonosov Moscow State University, Leninskie Gory, 1, build. 2, GSP-1, Moscow 119991, RussiaLong-term presence of M1 macrophages causes serious foreign body reaction (FBR), which is the main reason for the failure of biological scaffold integration. Inducing M2 polarization of macrophages near scaffolds to reduce foreign body response has been widely researched. In this work, inspired by the special capability of tumor exosomes in macrophages M2 polarization, we integrate tumor-derived exosomes into biological scaffolds to minimize the FBR. In brief, breast cancer cell-derived exosomes are loaded into polycaprolactone-b-polyethylene glycol-b-polycaprolactone (PCL-PEG-PCL) fiber scaffold through physical adsorption and entrapment to constructed bioactive engineered scaffold. In cellular experiments, we demonstrate bioactive engineered scaffold based on PCL-PEG-PCL and exosomes can promote the transformation of macrophages from M1 to M2 through the PI3K/Akt signaling pathway. In addition, the exosomes release gradually from scaffolds and act on the macrophages around the scaffolds to reduce FBR in a subcutaneous implant mouse model. Compared with PCL-PEG-PCL scaffolds without exosomes, bioactive engineered scaffolds reduce significantly inflammation and fibrosis of tissues around the scaffolds. Therefore, cancer cell-derived exosomes show the potential for constructing engineered scaffolds in inhibiting the excessive inflammation and facilitating tissue formation.http://www.sciencedirect.com/science/article/pii/S2666534422000174ExosomesMacrophagesInflammationPolarizationEngineered scaffolds |
| spellingShingle | Zehong Xiang Xinghua Guan Zhifang Ma Qiang Shi Mikhail Panteleev Fazly I. Ataullakhanov Bioactive engineered scaffolds based on PCL-PEG-PCL and tumor cell-derived exosomes to minimize the foreign body reaction Biomaterials and Biosystems Exosomes Macrophages Inflammation Polarization Engineered scaffolds |
| title | Bioactive engineered scaffolds based on PCL-PEG-PCL and tumor cell-derived exosomes to minimize the foreign body reaction |
| title_full | Bioactive engineered scaffolds based on PCL-PEG-PCL and tumor cell-derived exosomes to minimize the foreign body reaction |
| title_fullStr | Bioactive engineered scaffolds based on PCL-PEG-PCL and tumor cell-derived exosomes to minimize the foreign body reaction |
| title_full_unstemmed | Bioactive engineered scaffolds based on PCL-PEG-PCL and tumor cell-derived exosomes to minimize the foreign body reaction |
| title_short | Bioactive engineered scaffolds based on PCL-PEG-PCL and tumor cell-derived exosomes to minimize the foreign body reaction |
| title_sort | bioactive engineered scaffolds based on pcl peg pcl and tumor cell derived exosomes to minimize the foreign body reaction |
| topic | Exosomes Macrophages Inflammation Polarization Engineered scaffolds |
| url | http://www.sciencedirect.com/science/article/pii/S2666534422000174 |
| work_keys_str_mv | AT zehongxiang bioactiveengineeredscaffoldsbasedonpclpegpclandtumorcellderivedexosomestominimizetheforeignbodyreaction AT xinghuaguan bioactiveengineeredscaffoldsbasedonpclpegpclandtumorcellderivedexosomestominimizetheforeignbodyreaction AT zhifangma bioactiveengineeredscaffoldsbasedonpclpegpclandtumorcellderivedexosomestominimizetheforeignbodyreaction AT qiangshi bioactiveengineeredscaffoldsbasedonpclpegpclandtumorcellderivedexosomestominimizetheforeignbodyreaction AT mikhailpanteleev bioactiveengineeredscaffoldsbasedonpclpegpclandtumorcellderivedexosomestominimizetheforeignbodyreaction AT fazlyiataullakhanov bioactiveengineeredscaffoldsbasedonpclpegpclandtumorcellderivedexosomestominimizetheforeignbodyreaction |