Extraction Process Optimization and Antioxidant Activity of Green Tea Polysaccharides
Objective: To optimize the extraction process of green tea polysaccharide (GTP), purify the polysaccharide and analyze the antioxidant activity in vitro. Methods: The single factor experiments combined with response surface analysis was used to optimize the extraction process of polysaccharides. Hig...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-10-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021120287 |
| _version_ | 1811224424797437952 |
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| author | Yibin ZHANG Peng LIU Haiyu JI Xiaoyu JIA Ling TIAN |
| author_facet | Yibin ZHANG Peng LIU Haiyu JI Xiaoyu JIA Ling TIAN |
| author_sort | Yibin ZHANG |
| collection | DOAJ |
| description | Objective: To optimize the extraction process of green tea polysaccharide (GTP), purify the polysaccharide and analyze the antioxidant activity in vitro. Methods: The single factor experiments combined with response surface analysis was used to optimize the extraction process of polysaccharides. High performance gel permeation chromatography (HPGPC), gas chromatography (GC) and Fourier transform infrared spectroscopy (FTIR) were used to analyze the preliminary structure of polysaccharides, and the antioxidant activity of polysaccharides was studied by in vitro antioxidant experiments. Results: The optimal extraction conditions for GTP were determined as follows: Liquid/solid ratio 30:1 mL/g, extraction temperature 60 ℃, extraction time 70 min. Under these conditions, the yield of GTP reached to 10.56%. Structural analysis results showed that the total sugar, protein and uronic acid contents in GTP were 90.75%±3.69%, 0.92%±0.09%, and 0.82%±0.07%, respectively, and the average molecular weight was about 7.3 kDa. In vitro antioxidant test results showed that GTP had scavenging rates of 39.8%, 72.2% and 32.5% on ABTS+ free radical, DPPH free radical and hydroxyl free radical (OH·) at 2 mg/mL, respectively. Conclusion: In this process, green tea polysaccharides had presented high yield, low molecular weight and high antioxidant activity. |
| first_indexed | 2024-04-12T08:48:01Z |
| format | Article |
| id | doaj.art-2021a61d271744b19282326970fb9a38 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-12T08:48:01Z |
| publishDate | 2022-10-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-2021a61d271744b19282326970fb9a382022-12-22T03:39:38ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-10-01432022022710.13386/j.issn1002-0306.20211202872021120287-20Extraction Process Optimization and Antioxidant Activity of Green Tea PolysaccharidesYibin ZHANG0Peng LIU1Haiyu JI2Xiaoyu JIA3Ling TIAN4College of Biological Engineering, Tianjin Modern Vocational Technology College, Tianjin 300451, ChinaCollege of Biological Engineering, Tianjin Modern Vocational Technology College, Tianjin 300451, ChinaCollege of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, ChinaInstitute of Agricultural Products Preservation and Processing Technology, Tianjin Academy of Agricultural Sciences, Tianjin 300384, ChinaTangshan Shengchuan Agricultural Products Co., Ltd., Tangshan 064105, ChinaObjective: To optimize the extraction process of green tea polysaccharide (GTP), purify the polysaccharide and analyze the antioxidant activity in vitro. Methods: The single factor experiments combined with response surface analysis was used to optimize the extraction process of polysaccharides. High performance gel permeation chromatography (HPGPC), gas chromatography (GC) and Fourier transform infrared spectroscopy (FTIR) were used to analyze the preliminary structure of polysaccharides, and the antioxidant activity of polysaccharides was studied by in vitro antioxidant experiments. Results: The optimal extraction conditions for GTP were determined as follows: Liquid/solid ratio 30:1 mL/g, extraction temperature 60 ℃, extraction time 70 min. Under these conditions, the yield of GTP reached to 10.56%. Structural analysis results showed that the total sugar, protein and uronic acid contents in GTP were 90.75%±3.69%, 0.92%±0.09%, and 0.82%±0.07%, respectively, and the average molecular weight was about 7.3 kDa. In vitro antioxidant test results showed that GTP had scavenging rates of 39.8%, 72.2% and 32.5% on ABTS+ free radical, DPPH free radical and hydroxyl free radical (OH·) at 2 mg/mL, respectively. Conclusion: In this process, green tea polysaccharides had presented high yield, low molecular weight and high antioxidant activity.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021120287green tea polysaccharidesextraction processstructural analysisantioxidant activity |
| spellingShingle | Yibin ZHANG Peng LIU Haiyu JI Xiaoyu JIA Ling TIAN Extraction Process Optimization and Antioxidant Activity of Green Tea Polysaccharides Shipin gongye ke-ji green tea polysaccharides extraction process structural analysis antioxidant activity |
| title | Extraction Process Optimization and Antioxidant Activity of Green Tea Polysaccharides |
| title_full | Extraction Process Optimization and Antioxidant Activity of Green Tea Polysaccharides |
| title_fullStr | Extraction Process Optimization and Antioxidant Activity of Green Tea Polysaccharides |
| title_full_unstemmed | Extraction Process Optimization and Antioxidant Activity of Green Tea Polysaccharides |
| title_short | Extraction Process Optimization and Antioxidant Activity of Green Tea Polysaccharides |
| title_sort | extraction process optimization and antioxidant activity of green tea polysaccharides |
| topic | green tea polysaccharides extraction process structural analysis antioxidant activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021120287 |
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