Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells

The sperm of Russian sturgeon (Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833) was the research object. Acoustic-mechanical influence on sturgeon semen improves fish-biological and reproductive indices of the seminal fluid. The most optimal indices for reproductive cells of male Russian sturgeo...

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Main Authors: Ponomareva Elena, Firsova Angelina, Kovalenko Matvey, Polovinkina Mariya, Kuzov Anton, Alexandrova Uliyana, Pakhomov Victor
Format: Article
Language:English
Published: EDP Sciences 2023-01-01
Series:E3S Web of Conferences
Subjects:
Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/18/e3sconf_aquaculture2023_01074.pdf
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author Ponomareva Elena
Firsova Angelina
Kovalenko Matvey
Polovinkina Mariya
Kuzov Anton
Alexandrova Uliyana
Pakhomov Victor
author_facet Ponomareva Elena
Firsova Angelina
Kovalenko Matvey
Polovinkina Mariya
Kuzov Anton
Alexandrova Uliyana
Pakhomov Victor
author_sort Ponomareva Elena
collection DOAJ
description The sperm of Russian sturgeon (Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833) was the research object. Acoustic-mechanical influence on sturgeon semen improves fish-biological and reproductive indices of the seminal fluid. The most optimal indices for reproductive cells of male Russian sturgeon are 500 Hz, 90 V with the exposure duration of 1 minute. It has been shown that when a piezo-actuator with a frequency of 500 Hz and an amplitude of 90 V is applied to the sperm mixture with the cryoprotectant, ice crystals are formed later, which may influence the survival of cells after defrosting.
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spelling doaj.art-202423b4640246ec90dae9c57cf248862023-04-20T08:23:15ZengEDP SciencesE3S Web of Conferences2267-12422023-01-013810107410.1051/e3sconf/202338101074e3sconf_aquaculture2023_01074Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cellsPonomareva Elena0Firsova Angelina1Kovalenko Matvey2Polovinkina Mariya3Kuzov Anton4Alexandrova Uliyana5Pakhomov Victor6Federal Research Centre the Southern Scientific Centre of The Russian Academy of SciencesFederal Research Centre the Southern Scientific Centre of The Russian Academy of SciencesFederal Research Centre the Southern Scientific Centre of The Russian Academy of SciencesFederal Research Centre the Southern Scientific Centre of The Russian Academy of SciencesFederal Research Centre the Southern Scientific Centre of The Russian Academy of SciencesFederal Research Centre the Southern Scientific Centre of The Russian Academy of SciencesDon State Technical UniversityThe sperm of Russian sturgeon (Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833) was the research object. Acoustic-mechanical influence on sturgeon semen improves fish-biological and reproductive indices of the seminal fluid. The most optimal indices for reproductive cells of male Russian sturgeon are 500 Hz, 90 V with the exposure duration of 1 minute. It has been shown that when a piezo-actuator with a frequency of 500 Hz and an amplitude of 90 V is applied to the sperm mixture with the cryoprotectant, ice crystals are formed later, which may influence the survival of cells after defrosting.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/18/e3sconf_aquaculture2023_01074.pdfcryopreservationreproductive cells of fishsturgeonacoustic-mechanical impactfreezingcracking icemicroparticles
spellingShingle Ponomareva Elena
Firsova Angelina
Kovalenko Matvey
Polovinkina Mariya
Kuzov Anton
Alexandrova Uliyana
Pakhomov Victor
Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells
E3S Web of Conferences
cryopreservation
reproductive cells of fish
sturgeon
acoustic-mechanical impact
freezing
cracking ice
microparticles
title Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells
title_full Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells
title_fullStr Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells
title_full_unstemmed Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells
title_short Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells
title_sort application of piezoactuators in the technology of low temperature preservation of fish reproductive cells
topic cryopreservation
reproductive cells of fish
sturgeon
acoustic-mechanical impact
freezing
cracking ice
microparticles
url https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/18/e3sconf_aquaculture2023_01074.pdf
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