Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells
The sperm of Russian sturgeon (Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833) was the research object. Acoustic-mechanical influence on sturgeon semen improves fish-biological and reproductive indices of the seminal fluid. The most optimal indices for reproductive cells of male Russian sturgeo...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
EDP Sciences
2023-01-01
|
Series: | E3S Web of Conferences |
Subjects: | |
Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/18/e3sconf_aquaculture2023_01074.pdf |
_version_ | 1797843622891094016 |
---|---|
author | Ponomareva Elena Firsova Angelina Kovalenko Matvey Polovinkina Mariya Kuzov Anton Alexandrova Uliyana Pakhomov Victor |
author_facet | Ponomareva Elena Firsova Angelina Kovalenko Matvey Polovinkina Mariya Kuzov Anton Alexandrova Uliyana Pakhomov Victor |
author_sort | Ponomareva Elena |
collection | DOAJ |
description | The sperm of Russian sturgeon (Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833) was the research object. Acoustic-mechanical influence on sturgeon semen improves fish-biological and reproductive indices of the seminal fluid. The most optimal indices for reproductive cells of male Russian sturgeon are 500 Hz, 90 V with the exposure duration of 1 minute. It has been shown that when a piezo-actuator with a frequency of 500 Hz and an amplitude of 90 V is applied to the sperm mixture with the cryoprotectant, ice crystals are formed later, which may influence the survival of cells after defrosting. |
first_indexed | 2024-04-09T17:08:16Z |
format | Article |
id | doaj.art-202423b4640246ec90dae9c57cf24886 |
institution | Directory Open Access Journal |
issn | 2267-1242 |
language | English |
last_indexed | 2024-04-09T17:08:16Z |
publishDate | 2023-01-01 |
publisher | EDP Sciences |
record_format | Article |
series | E3S Web of Conferences |
spelling | doaj.art-202423b4640246ec90dae9c57cf248862023-04-20T08:23:15ZengEDP SciencesE3S Web of Conferences2267-12422023-01-013810107410.1051/e3sconf/202338101074e3sconf_aquaculture2023_01074Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cellsPonomareva Elena0Firsova Angelina1Kovalenko Matvey2Polovinkina Mariya3Kuzov Anton4Alexandrova Uliyana5Pakhomov Victor6Federal Research Centre the Southern Scientific Centre of The Russian Academy of SciencesFederal Research Centre the Southern Scientific Centre of The Russian Academy of SciencesFederal Research Centre the Southern Scientific Centre of The Russian Academy of SciencesFederal Research Centre the Southern Scientific Centre of The Russian Academy of SciencesFederal Research Centre the Southern Scientific Centre of The Russian Academy of SciencesFederal Research Centre the Southern Scientific Centre of The Russian Academy of SciencesDon State Technical UniversityThe sperm of Russian sturgeon (Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833) was the research object. Acoustic-mechanical influence on sturgeon semen improves fish-biological and reproductive indices of the seminal fluid. The most optimal indices for reproductive cells of male Russian sturgeon are 500 Hz, 90 V with the exposure duration of 1 minute. It has been shown that when a piezo-actuator with a frequency of 500 Hz and an amplitude of 90 V is applied to the sperm mixture with the cryoprotectant, ice crystals are formed later, which may influence the survival of cells after defrosting.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/18/e3sconf_aquaculture2023_01074.pdfcryopreservationreproductive cells of fishsturgeonacoustic-mechanical impactfreezingcracking icemicroparticles |
spellingShingle | Ponomareva Elena Firsova Angelina Kovalenko Matvey Polovinkina Mariya Kuzov Anton Alexandrova Uliyana Pakhomov Victor Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells E3S Web of Conferences cryopreservation reproductive cells of fish sturgeon acoustic-mechanical impact freezing cracking ice microparticles |
title | Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells |
title_full | Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells |
title_fullStr | Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells |
title_full_unstemmed | Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells |
title_short | Application of piezoactuators in the technology of low-temperature preservation of fish reproductive cells |
title_sort | application of piezoactuators in the technology of low temperature preservation of fish reproductive cells |
topic | cryopreservation reproductive cells of fish sturgeon acoustic-mechanical impact freezing cracking ice microparticles |
url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/18/e3sconf_aquaculture2023_01074.pdf |
work_keys_str_mv | AT ponomarevaelena applicationofpiezoactuatorsinthetechnologyoflowtemperaturepreservationoffishreproductivecells AT firsovaangelina applicationofpiezoactuatorsinthetechnologyoflowtemperaturepreservationoffishreproductivecells AT kovalenkomatvey applicationofpiezoactuatorsinthetechnologyoflowtemperaturepreservationoffishreproductivecells AT polovinkinamariya applicationofpiezoactuatorsinthetechnologyoflowtemperaturepreservationoffishreproductivecells AT kuzovanton applicationofpiezoactuatorsinthetechnologyoflowtemperaturepreservationoffishreproductivecells AT alexandrovauliyana applicationofpiezoactuatorsinthetechnologyoflowtemperaturepreservationoffishreproductivecells AT pakhomovvictor applicationofpiezoactuatorsinthetechnologyoflowtemperaturepreservationoffishreproductivecells |