Functional characterisation of semen in honeybee queen (A.m.ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination

Differences over time in the quality of semen present in the honey bee (Apis mellifera ligustica) queen spermatheca were<br />studied. An increase in the non-vital spermatozoa was shown to be evident (P>0.05) between the 12th and 24th month.<br />The study of semen viability demonstra...

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Main Authors: Andrea Galli, Donatella Balduzzi, Marco Lodesani
Format: Article
Language:English
Published: Taylor & Francis Group 2010-01-01
Series:Italian Journal of Animal Science
Subjects:
Online Access:http://www.aspajournal.it/index.php/ijas/article/view/214
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author Andrea Galli
Donatella Balduzzi
Marco Lodesani
author_facet Andrea Galli
Donatella Balduzzi
Marco Lodesani
author_sort Andrea Galli
collection DOAJ
description Differences over time in the quality of semen present in the honey bee (Apis mellifera ligustica) queen spermatheca were<br />studied. An increase in the non-vital spermatozoa was shown to be evident (P>0.05) between the 12th and 24th month.<br />The study of semen viability demonstrated that the passage of the semen to the spermatheca is due to sperm motility.<br />In the queen inseminated with non-viable spermatozoa, no semen was detected in the spermatheca. Queens inseminated<br />twice with a Hyes solution/semen mixture (1:1) stored as many spermatozoa in their spermatheca as those inseminated<br />once with the classic technique. Queen replacement, oviposition and other functional characteristics were similar<br />to those observed in the classic insemination procedure.
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spelling doaj.art-20272ebb22154252ae9faba3933b0f9c2022-12-22T03:54:44ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2010-01-013438539210.4081/ijas.2004.385Functional characterisation of semen in honeybee queen (A.m.ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental inseminationAndrea GalliDonatella BalduzziMarco LodesaniDifferences over time in the quality of semen present in the honey bee (Apis mellifera ligustica) queen spermatheca were<br />studied. An increase in the non-vital spermatozoa was shown to be evident (P>0.05) between the 12th and 24th month.<br />The study of semen viability demonstrated that the passage of the semen to the spermatheca is due to sperm motility.<br />In the queen inseminated with non-viable spermatozoa, no semen was detected in the spermatheca. Queens inseminated<br />twice with a Hyes solution/semen mixture (1:1) stored as many spermatozoa in their spermatheca as those inseminated<br />once with the classic technique. Queen replacement, oviposition and other functional characteristics were similar<br />to those observed in the classic insemination procedure.http://www.aspajournal.it/index.php/ijas/article/view/214Apis mellifera ligustica, Queen honey bee, Spermatheca, Spermatozoa, Instrumental insemination
spellingShingle Andrea Galli
Donatella Balduzzi
Marco Lodesani
Functional characterisation of semen in honeybee queen (A.m.ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination
Italian Journal of Animal Science
Apis mellifera ligustica, Queen honey bee, Spermatheca, Spermatozoa, Instrumental insemination
title Functional characterisation of semen in honeybee queen (A.m.ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination
title_full Functional characterisation of semen in honeybee queen (A.m.ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination
title_fullStr Functional characterisation of semen in honeybee queen (A.m.ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination
title_full_unstemmed Functional characterisation of semen in honeybee queen (A.m.ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination
title_short Functional characterisation of semen in honeybee queen (A.m.ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination
title_sort functional characterisation of semen in honeybee queen a m ligustica s spermatheca and efficiency of the diluted semen technique in instrumental insemination
topic Apis mellifera ligustica, Queen honey bee, Spermatheca, Spermatozoa, Instrumental insemination
url http://www.aspajournal.it/index.php/ijas/article/view/214
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