Lack of expression of hepatitis C virus core protein in human monocyte-erived dendritic cells using recombinant semliki forest virus
Hepatitis C Virus belongs to the Flaviviridae family. One proposed mechanism of HCV persistence in the ability to infect hematopoietic cells, including Dendritic cells (DCs). HCV infection of DCs could impair their functions that represent one of the mechanisms, thus hampering viral clearance by th...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universidad Nacional de Colombia
2019-09-01
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| Series: | Acta Biológica Colombiana |
| Subjects: | |
| Online Access: | https://revistas.unal.edu.co/index.php/actabiol/article/view/79368 |
| _version_ | 1818789328687464448 |
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| author | Maria-Cristina Navas Françoise Stoll-Keller Jovan Pavlovic |
| author_facet | Maria-Cristina Navas Françoise Stoll-Keller Jovan Pavlovic |
| author_sort | Maria-Cristina Navas |
| collection | DOAJ |
| description | Hepatitis C Virus belongs to the Flaviviridae family. One proposed mechanism of HCV persistence in the ability to infect hematopoietic cells, including Dendritic cells (DCs). HCV infection of DCs could impair their functions that represent one of the mechanisms, thus hampering viral clearance by the host immune system. Among HCV-encoded proteins, the highly conserved Core protein has been suggested to be responsible for the immunomodulatory properties of this Hepacivirus. Recombinant viral vectors expressing the HCV Core protein and allowing its transduction and therefore the expression of the protein into DCs could be useful tools for the analysis of the properties of the Core protein. Vaccinia Virus and retrovirus have been used to transduce human DCs. Likewise, gene transfer into DCs using Semliki Forest Virus has been reported. This study aimed to express the HCV Core protein in human monocyte-derived DCs using an SFV vector, in which the subgenomic RNA encoding the structural proteins was replaced by the HCV Core sequence and then analyze the effects of its expression on DCs functions. |
| first_indexed | 2024-12-18T14:37:49Z |
| format | Article |
| id | doaj.art-202b3f2b0d034e03a96462f159cf1f30 |
| institution | Directory Open Access Journal |
| issn | 0120-548X 1900-1649 |
| language | English |
| last_indexed | 2024-12-18T14:37:49Z |
| publishDate | 2019-09-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Acta Biológica Colombiana |
| spelling | doaj.art-202b3f2b0d034e03a96462f159cf1f302022-12-21T21:04:28ZengUniversidad Nacional de ColombiaActa Biológica Colombiana0120-548X1900-16492019-09-0124310.15446/abc.v24n3.7936851439Lack of expression of hepatitis C virus core protein in human monocyte-erived dendritic cells using recombinant semliki forest virusMaria-Cristina Navas0Françoise Stoll-Keller1Jovan Pavlovic2Universidad de Antioquia - Facultad de Medicina - Grupo GastrohepatologíaStrasbourg University Hospitals - Virology LaboratoryUniversity of Zürich - Institute of Medical VirologyHepatitis C Virus belongs to the Flaviviridae family. One proposed mechanism of HCV persistence in the ability to infect hematopoietic cells, including Dendritic cells (DCs). HCV infection of DCs could impair their functions that represent one of the mechanisms, thus hampering viral clearance by the host immune system. Among HCV-encoded proteins, the highly conserved Core protein has been suggested to be responsible for the immunomodulatory properties of this Hepacivirus. Recombinant viral vectors expressing the HCV Core protein and allowing its transduction and therefore the expression of the protein into DCs could be useful tools for the analysis of the properties of the Core protein. Vaccinia Virus and retrovirus have been used to transduce human DCs. Likewise, gene transfer into DCs using Semliki Forest Virus has been reported. This study aimed to express the HCV Core protein in human monocyte-derived DCs using an SFV vector, in which the subgenomic RNA encoding the structural proteins was replaced by the HCV Core sequence and then analyze the effects of its expression on DCs functions.https://revistas.unal.edu.co/index.php/actabiol/article/view/79368Dendritic cellsHCVSFVviral vector |
| spellingShingle | Maria-Cristina Navas Françoise Stoll-Keller Jovan Pavlovic Lack of expression of hepatitis C virus core protein in human monocyte-erived dendritic cells using recombinant semliki forest virus Acta Biológica Colombiana Dendritic cells HCV SFV viral vector |
| title | Lack of expression of hepatitis C virus core protein in human monocyte-erived dendritic cells using recombinant semliki forest virus |
| title_full | Lack of expression of hepatitis C virus core protein in human monocyte-erived dendritic cells using recombinant semliki forest virus |
| title_fullStr | Lack of expression of hepatitis C virus core protein in human monocyte-erived dendritic cells using recombinant semliki forest virus |
| title_full_unstemmed | Lack of expression of hepatitis C virus core protein in human monocyte-erived dendritic cells using recombinant semliki forest virus |
| title_short | Lack of expression of hepatitis C virus core protein in human monocyte-erived dendritic cells using recombinant semliki forest virus |
| title_sort | lack of expression of hepatitis c virus core protein in human monocyte erived dendritic cells using recombinant semliki forest virus |
| topic | Dendritic cells HCV SFV viral vector |
| url | https://revistas.unal.edu.co/index.php/actabiol/article/view/79368 |
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