Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles
Although nanotoxicology has become a large research field, assessment of cytotoxicity is often reduced to analysis of one cell line only. Cytotoxicity of nanoparticles is complex and should, preferentially, be evaluated in several cell lines with different methods and on multiple nanoparticle batche...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/18/11/2454 |
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| author | Einar Sulheim Tore-Geir Iversen Vu To Nakstad Geir Klinkenberg Håvard Sletta Ruth Schmid Anne Rein Hatletveit Ane Marit Wågbø Anders Sundan Tore Skotland Kirsten Sandvig Ýrr Mørch |
| author_facet | Einar Sulheim Tore-Geir Iversen Vu To Nakstad Geir Klinkenberg Håvard Sletta Ruth Schmid Anne Rein Hatletveit Ane Marit Wågbø Anders Sundan Tore Skotland Kirsten Sandvig Ýrr Mørch |
| author_sort | Einar Sulheim |
| collection | DOAJ |
| description | Although nanotoxicology has become a large research field, assessment of cytotoxicity is often reduced to analysis of one cell line only. Cytotoxicity of nanoparticles is complex and should, preferentially, be evaluated in several cell lines with different methods and on multiple nanoparticle batches. Here we report the toxicity of poly(alkyl cyanoacrylate) nanoparticles in 12 different cell lines after synthesizing and analyzing 19 different nanoparticle batches and report that large variations were obtained when using different cell lines or various toxicity assays. Surprisingly, we found that nanoparticles with intermediate degradation rates were less toxic than particles that were degraded faster or more slowly in a cell-free system. The toxicity did not vary significantly with either the three different combinations of polyethylene glycol surfactants or with particle size (range 100–200 nm). No acute pro- or anti-inflammatory activity on cells in whole blood was observed. |
| first_indexed | 2024-04-13T00:19:55Z |
| format | Article |
| id | doaj.art-202d45f8c0644fa68206a5cb1e219976 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T00:19:55Z |
| publishDate | 2017-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-202d45f8c0644fa68206a5cb1e2199762022-12-22T03:10:49ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-11-011811245410.3390/ijms18112454ijms18112454Cytotoxicity of Poly(Alkyl Cyanoacrylate) NanoparticlesEinar Sulheim0Tore-Geir Iversen1Vu To Nakstad2Geir Klinkenberg3Håvard Sletta4Ruth Schmid5Anne Rein Hatletveit6Ane Marit Wågbø7Anders Sundan8Tore Skotland9Kirsten Sandvig10Ýrr Mørch11SINTEF Materials and Chemistry, Sem Sælands vei 2A, 7034 Trondheim, NorwayDepartment of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital—The Norwegian Radium Hospital, 0379 Oslo, NorwaySINTEF Materials and Chemistry, Sem Sælands vei 2A, 7034 Trondheim, NorwaySINTEF Materials and Chemistry, Sem Sælands vei 2A, 7034 Trondheim, NorwaySINTEF Materials and Chemistry, Sem Sælands vei 2A, 7034 Trondheim, NorwaySINTEF Materials and Chemistry, Sem Sælands vei 2A, 7034 Trondheim, NorwaySINTEF Materials and Chemistry, Sem Sælands vei 2A, 7034 Trondheim, NorwaySINTEF Materials and Chemistry, Sem Sælands vei 2A, 7034 Trondheim, NorwayDepartment of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, 8905 MH, 7491 Trondheim, NorwayDepartment of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital—The Norwegian Radium Hospital, 0379 Oslo, NorwayDepartment of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital—The Norwegian Radium Hospital, 0379 Oslo, NorwaySINTEF Materials and Chemistry, Sem Sælands vei 2A, 7034 Trondheim, NorwayAlthough nanotoxicology has become a large research field, assessment of cytotoxicity is often reduced to analysis of one cell line only. Cytotoxicity of nanoparticles is complex and should, preferentially, be evaluated in several cell lines with different methods and on multiple nanoparticle batches. Here we report the toxicity of poly(alkyl cyanoacrylate) nanoparticles in 12 different cell lines after synthesizing and analyzing 19 different nanoparticle batches and report that large variations were obtained when using different cell lines or various toxicity assays. Surprisingly, we found that nanoparticles with intermediate degradation rates were less toxic than particles that were degraded faster or more slowly in a cell-free system. The toxicity did not vary significantly with either the three different combinations of polyethylene glycol surfactants or with particle size (range 100–200 nm). No acute pro- or anti-inflammatory activity on cells in whole blood was observed.https://www.mdpi.com/1422-0067/18/11/2454nanoparticlesPACAcytotoxicitynanotoxicologyhigh-throughput screening |
| spellingShingle | Einar Sulheim Tore-Geir Iversen Vu To Nakstad Geir Klinkenberg Håvard Sletta Ruth Schmid Anne Rein Hatletveit Ane Marit Wågbø Anders Sundan Tore Skotland Kirsten Sandvig Ýrr Mørch Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles International Journal of Molecular Sciences nanoparticles PACA cytotoxicity nanotoxicology high-throughput screening |
| title | Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles |
| title_full | Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles |
| title_fullStr | Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles |
| title_full_unstemmed | Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles |
| title_short | Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles |
| title_sort | cytotoxicity of poly alkyl cyanoacrylate nanoparticles |
| topic | nanoparticles PACA cytotoxicity nanotoxicology high-throughput screening |
| url | https://www.mdpi.com/1422-0067/18/11/2454 |
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