Extractive Spectrophotometric Determination of Nortriptyline Hydrochloride Using Sudan II, IV and Black B
A simple spectrophotometric methods has been developed for the determination of nortriptyline hydrochloride in pure and in pharmaceutical formulations based on the formation of ion-pair complexes with sudun II (SII), sudan (IV) (SIV) and sudan black B (SBB). The selectivity of the method was improve...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-08-01
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| Series: | Scientia Pharmaceutica |
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| Online Access: | http://www.mdpi.com/2218-0532/69/2/16 |
| _version_ | 1798039494940688384 |
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| author | A. S. Amin H. M. Saleh |
| author_facet | A. S. Amin H. M. Saleh |
| author_sort | A. S. Amin |
| collection | DOAJ |
| description | A simple spectrophotometric methods has been developed for the determination of nortriptyline hydrochloride in pure and in pharmaceutical
formulations based on the formation of ion-pair complexes with sudun II (SII), sudan (IV) (SIV) and sudan black B (SBB). The selectivity of the method was improved through extraction with chloroform. The optimum conditions for complete extracted colour development were assessed. The absorbance measurements were made at 534, 596 and 649 nm for SII, SIV and SBB complexes, respectively. The calibration graph was linear in the ranges 0.5- 280. 0.5- 37.5 and 0.5 – 31.0 μg ml−1 of the drug usiny the same reagents, respectively. The precision of the procedure was checked by calculating the relative standard deviation of ten replicate determinations on 15 μg ml−1 of nortriptyline HCI and was found to be 1.7, 1.3 and 1.55% using SII, SIV, and SBB complexes, respectively. The molar absorptivity and Sandell sensitivity for each ion-pair were calculated. The proposed methods were successfully applied to the deterniination of pure nortriptyline HCI and in pharmaceutical formulations, and the results demonstrated that the method is equally accurate, precise and reproducible as the official method. |
| first_indexed | 2024-04-11T21:54:40Z |
| format | Article |
| id | doaj.art-202ea72f32a6450987e47a4f5a7f8e8e |
| institution | Directory Open Access Journal |
| issn | 2218-0532 |
| language | English |
| last_indexed | 2024-04-11T21:54:40Z |
| publishDate | 2017-08-01 |
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| series | Scientia Pharmaceutica |
| spelling | doaj.art-202ea72f32a6450987e47a4f5a7f8e8e2022-12-22T04:01:08ZengMDPI AGScientia Pharmaceutica2218-05322017-08-0169214014910.3797/scipharm.aut-01-16scipharm.aut-01-16Extractive Spectrophotometric Determination of Nortriptyline Hydrochloride Using Sudan II, IV and Black BA. S. Amin0H. M. Saleh1Chemistry Department, Faculty of Science, Benha University, Benha, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig, EgyptA simple spectrophotometric methods has been developed for the determination of nortriptyline hydrochloride in pure and in pharmaceutical formulations based on the formation of ion-pair complexes with sudun II (SII), sudan (IV) (SIV) and sudan black B (SBB). The selectivity of the method was improved through extraction with chloroform. The optimum conditions for complete extracted colour development were assessed. The absorbance measurements were made at 534, 596 and 649 nm for SII, SIV and SBB complexes, respectively. The calibration graph was linear in the ranges 0.5- 280. 0.5- 37.5 and 0.5 – 31.0 μg ml−1 of the drug usiny the same reagents, respectively. The precision of the procedure was checked by calculating the relative standard deviation of ten replicate determinations on 15 μg ml−1 of nortriptyline HCI and was found to be 1.7, 1.3 and 1.55% using SII, SIV, and SBB complexes, respectively. The molar absorptivity and Sandell sensitivity for each ion-pair were calculated. The proposed methods were successfully applied to the deterniination of pure nortriptyline HCI and in pharmaceutical formulations, and the results demonstrated that the method is equally accurate, precise and reproducible as the official method.http://www.mdpi.com/2218-0532/69/2/16Nortriptyline HCISudan II, IV and Black Bdosage forms |
| spellingShingle | A. S. Amin H. M. Saleh Extractive Spectrophotometric Determination of Nortriptyline Hydrochloride Using Sudan II, IV and Black B Scientia Pharmaceutica Nortriptyline HCI Sudan II, IV and Black B dosage forms |
| title | Extractive Spectrophotometric Determination of Nortriptyline Hydrochloride Using Sudan II, IV and Black B |
| title_full | Extractive Spectrophotometric Determination of Nortriptyline Hydrochloride Using Sudan II, IV and Black B |
| title_fullStr | Extractive Spectrophotometric Determination of Nortriptyline Hydrochloride Using Sudan II, IV and Black B |
| title_full_unstemmed | Extractive Spectrophotometric Determination of Nortriptyline Hydrochloride Using Sudan II, IV and Black B |
| title_short | Extractive Spectrophotometric Determination of Nortriptyline Hydrochloride Using Sudan II, IV and Black B |
| title_sort | extractive spectrophotometric determination of nortriptyline hydrochloride using sudan ii iv and black b |
| topic | Nortriptyline HCI Sudan II, IV and Black B dosage forms |
| url | http://www.mdpi.com/2218-0532/69/2/16 |
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