ZBTB20 promotes cell migration and invasion of gastric cancer by inhibiting IκBα to induce NF-κB activation
Zinc finger and BTB domain containing 20 (ZBTB20), a sequence-specific transcriptional repressor, has been found to be involved in tumorigenesis. However, its role(s) in gastric cancer and the molecular mechanisms involved are poorly investigated. Here, our data demonstrated that ZBTB20 expression w...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1670188 |
| _version_ | 1818140616317468672 |
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| author | Yangmei Zhang Xichang Zhou ManMan Zhang Long Cheng Youwei Zhang Xiang Wang |
| author_facet | Yangmei Zhang Xichang Zhou ManMan Zhang Long Cheng Youwei Zhang Xiang Wang |
| author_sort | Yangmei Zhang |
| collection | DOAJ |
| description | Zinc finger and BTB domain containing 20 (ZBTB20), a sequence-specific transcriptional repressor, has been found to be involved in tumorigenesis. However, its role(s) in gastric cancer and the molecular mechanisms involved are poorly investigated. Here, our data demonstrated that ZBTB20 expression was markedly upregulated in gastric cancer cell lines infected with Helicobacter pylori (H. pylori) and in gastric cancer tumor samples. Loss- and gain-of-function studies showed that ZBTB20 promoted cell proliferation, invasion and migration of gastric cancer cell lines. Mechanistically, the phosphorylation of NF-κBp65 and expression and activity of MMP-2 and MMP-9 were increased, while IκBα expression was decreased by ZBTB20 in gastric cancer cells. We further revealed that IκBα overexpression significantly inhibited NF-κB signaling as well as cell migration, invasion and proliferation in gastric cancer cell lines induced by ZBTB20 overexpression. Therefore, our findings emphasize an important role for ZBTB20 in controlling gastric cancer development, which is helpful to identify potential therapeutic targets for its treatment. |
| first_indexed | 2024-12-11T10:46:49Z |
| format | Article |
| id | doaj.art-2032056249bb4abea2a03035e8014c9a |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-12-11T10:46:49Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-2032056249bb4abea2a03035e8014c9a2022-12-22T01:10:26ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014713862387210.1080/21691401.2019.1670188ZBTB20 promotes cell migration and invasion of gastric cancer by inhibiting IκBα to induce NF-κB activationYangmei Zhang0Xichang Zhou1ManMan Zhang2Long Cheng3Youwei Zhang4Xiang Wang5Department of Oncology, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, ChinaDepartment of Intervention, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, ChinaDepartment of Digestion, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, ChinaDepartment of Intervention, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, ChinaDepartment of Oncology, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, ChinaDepartment of Oncology, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, ChinaZinc finger and BTB domain containing 20 (ZBTB20), a sequence-specific transcriptional repressor, has been found to be involved in tumorigenesis. However, its role(s) in gastric cancer and the molecular mechanisms involved are poorly investigated. Here, our data demonstrated that ZBTB20 expression was markedly upregulated in gastric cancer cell lines infected with Helicobacter pylori (H. pylori) and in gastric cancer tumor samples. Loss- and gain-of-function studies showed that ZBTB20 promoted cell proliferation, invasion and migration of gastric cancer cell lines. Mechanistically, the phosphorylation of NF-κBp65 and expression and activity of MMP-2 and MMP-9 were increased, while IκBα expression was decreased by ZBTB20 in gastric cancer cells. We further revealed that IκBα overexpression significantly inhibited NF-κB signaling as well as cell migration, invasion and proliferation in gastric cancer cell lines induced by ZBTB20 overexpression. Therefore, our findings emphasize an important role for ZBTB20 in controlling gastric cancer development, which is helpful to identify potential therapeutic targets for its treatment.https://www.tandfonline.com/doi/10.1080/21691401.2019.1670188Gastric cancerZBTB20IκBαNF-κBp65 |
| spellingShingle | Yangmei Zhang Xichang Zhou ManMan Zhang Long Cheng Youwei Zhang Xiang Wang ZBTB20 promotes cell migration and invasion of gastric cancer by inhibiting IκBα to induce NF-κB activation Artificial Cells, Nanomedicine, and Biotechnology Gastric cancer ZBTB20 IκBα NF-κBp65 |
| title | ZBTB20 promotes cell migration and invasion of gastric cancer by inhibiting IκBα to induce NF-κB activation |
| title_full | ZBTB20 promotes cell migration and invasion of gastric cancer by inhibiting IκBα to induce NF-κB activation |
| title_fullStr | ZBTB20 promotes cell migration and invasion of gastric cancer by inhibiting IκBα to induce NF-κB activation |
| title_full_unstemmed | ZBTB20 promotes cell migration and invasion of gastric cancer by inhibiting IκBα to induce NF-κB activation |
| title_short | ZBTB20 promotes cell migration and invasion of gastric cancer by inhibiting IκBα to induce NF-κB activation |
| title_sort | zbtb20 promotes cell migration and invasion of gastric cancer by inhibiting iκbα to induce nf κb activation |
| topic | Gastric cancer ZBTB20 IκBα NF-κBp65 |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1670188 |
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