CD44 is overexpressed and correlated with tumor progression in gallbladder cancer
Yuting He,1,2,* Chen Xue,1,2,* Yan Yu,1,2 Jianan Chen,1,2 Xiaolong Chen,1,2 Fang Ren,2 Zhigang Ren,1,2 Guangying Cui,1,2 Ranran Sun1,2 1Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; 2Key Laboratory of Clinical Medicine, The First Af...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2018-09-01
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| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/cd44-is-overexpressed-and-correlated-with-tumor-progression-in-gallbla-peer-reviewed-article-CMAR |
| _version_ | 1819262639748939776 |
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| author | He YT Xue C Yu Y Chen JA Chen XL Ren F Ren ZG Cui GY Sun RR |
| author_facet | He YT Xue C Yu Y Chen JA Chen XL Ren F Ren ZG Cui GY Sun RR |
| author_sort | He YT |
| collection | DOAJ |
| description | Yuting He,1,2,* Chen Xue,1,2,* Yan Yu,1,2 Jianan Chen,1,2 Xiaolong Chen,1,2 Fang Ren,2 Zhigang Ren,1,2 Guangying Cui,1,2 Ranran Sun1,2 1Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; 2Key Laboratory of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China *These authors contributed equally to this work Background: Gallbladder cancer (GBC) is a highly lethal disease and the most common biliary tract malignant tumor with poor prognosis. Accumulating evidence indicates that cluster of differentiation 44 (CD44) is overexpressed in several malignancies and has a crucial role in the development of cancer. However, its expression and function in GBC are unclear. The aim of this study was to explore CD44 expression and its role in GBC. Materials and methods: The expression of CD44 was measured by immunohistochemistry. Tissue microarray analysis was used to confirm the relationship between CD44 expression and clinical outcomes of GBC patients. EDU assay, colony formation assay, cell migration and invasion assay were performed to detect the functions of CD44 in GBC-SD and NOZ transfected with si-RNA. Results: CD44 was overexpressed and associated with poor outcomes in GBC patients. The univariate and multivariate analyses confirmed that elevated CD44 was an independent prognostic factor for the OS of GBC patients. Silencing CD44 could suppress the GBC cell proliferation, migration and invasion in vitro, as well as attenuated cancer stem cell functions. Conclusion: CD44 markedly correlated with aggressive tumor behaviors and contributed to the progression of GBC, which could represent a novel prognostic marker and potential therapeutic target for GBC patients. Keywords: CD44, gallbladder cancer, cancer stem cells, tumor progression |
| first_indexed | 2024-12-23T20:00:54Z |
| format | Article |
| id | doaj.art-203697a3c6a84d80a206d8f24a896187 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-23T20:00:54Z |
| publishDate | 2018-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-203697a3c6a84d80a206d8f24a8961872022-12-21T17:33:04ZengDove Medical PressCancer Management and Research1179-13222018-09-01Volume 103857386540822CD44 is overexpressed and correlated with tumor progression in gallbladder cancerHe YTXue CYu YChen JAChen XLRen FRen ZGCui GYSun RRYuting He,1,2,* Chen Xue,1,2,* Yan Yu,1,2 Jianan Chen,1,2 Xiaolong Chen,1,2 Fang Ren,2 Zhigang Ren,1,2 Guangying Cui,1,2 Ranran Sun1,2 1Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; 2Key Laboratory of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China *These authors contributed equally to this work Background: Gallbladder cancer (GBC) is a highly lethal disease and the most common biliary tract malignant tumor with poor prognosis. Accumulating evidence indicates that cluster of differentiation 44 (CD44) is overexpressed in several malignancies and has a crucial role in the development of cancer. However, its expression and function in GBC are unclear. The aim of this study was to explore CD44 expression and its role in GBC. Materials and methods: The expression of CD44 was measured by immunohistochemistry. Tissue microarray analysis was used to confirm the relationship between CD44 expression and clinical outcomes of GBC patients. EDU assay, colony formation assay, cell migration and invasion assay were performed to detect the functions of CD44 in GBC-SD and NOZ transfected with si-RNA. Results: CD44 was overexpressed and associated with poor outcomes in GBC patients. The univariate and multivariate analyses confirmed that elevated CD44 was an independent prognostic factor for the OS of GBC patients. Silencing CD44 could suppress the GBC cell proliferation, migration and invasion in vitro, as well as attenuated cancer stem cell functions. Conclusion: CD44 markedly correlated with aggressive tumor behaviors and contributed to the progression of GBC, which could represent a novel prognostic marker and potential therapeutic target for GBC patients. Keywords: CD44, gallbladder cancer, cancer stem cells, tumor progressionhttps://www.dovepress.com/cd44-is-overexpressed-and-correlated-with-tumor-progression-in-gallbla-peer-reviewed-article-CMARCD44gallbladder cancercancer stem cellstumor progression |
| spellingShingle | He YT Xue C Yu Y Chen JA Chen XL Ren F Ren ZG Cui GY Sun RR CD44 is overexpressed and correlated with tumor progression in gallbladder cancer Cancer Management and Research CD44 gallbladder cancer cancer stem cells tumor progression |
| title | CD44 is overexpressed and correlated with tumor progression in gallbladder cancer |
| title_full | CD44 is overexpressed and correlated with tumor progression in gallbladder cancer |
| title_fullStr | CD44 is overexpressed and correlated with tumor progression in gallbladder cancer |
| title_full_unstemmed | CD44 is overexpressed and correlated with tumor progression in gallbladder cancer |
| title_short | CD44 is overexpressed and correlated with tumor progression in gallbladder cancer |
| title_sort | cd44 is overexpressed and correlated with tumor progression in gallbladder cancer |
| topic | CD44 gallbladder cancer cancer stem cells tumor progression |
| url | https://www.dovepress.com/cd44-is-overexpressed-and-correlated-with-tumor-progression-in-gallbla-peer-reviewed-article-CMAR |
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