Rapid Immunochromatographic Test for the Identification and Discrimination of Mycobacterium tuberculosis Complex Isolates from Non-tuberculous Mycobacteria
Background: A new rapid Immunochromatographic test (ICT) kit (SDBioline TB Ag MPT64RAPID®) developed by Standard Diagnostics, South Korea was evaluated for rapid differentiation of M. tuberculosis from non tuberculous mycobacteria (NTM). It detects MPT 64 antigen in M. tuberculosis isolates usin...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2014-04-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/4253/7098_E(C)_F(T)_PF1(PAK)_PFA(P)_PF2(PN).pdf |
| Summary: | Background: A new rapid Immunochromatographic test (ICT)
kit (SDBioline TB Ag MPT64RAPID®) developed by Standard
Diagnostics, South Korea was evaluated for rapid differentiation
of M. tuberculosis from non tuberculous mycobacteria (NTM). It
detects MPT 64 antigen in M. tuberculosis isolates using mouse
monoclonal MPT 64 antibody. The kit was assessed for routine
identification of the Acid Fast Bacilli(AFB) isolated in our laboratory.
Materials and Methods: Two hundred eight culture isolates
of Mycobacteria were tested using ICT test kit for detection of
MPT 64 antigen from liquid and solid culture. H37Rv strain was
employed as the positive reference control. Any negative result
was referred for confirmation by Gen Probe Accu Probe assay
for MTB Complex (Gen-Probe, San Diego, Calif.). Speciation of
NTM was performed using genotypic Mycobacterium CM assay
(Hain’s life sciences, Germany).
Results: Of the 208 culture positive isolates tested, 182 (87.5%)
were found positive for Mycobacterium tuberculosis Complex
and remaining 26 (12.5%) were considered as NTM. These
results were further confirmed by Gen Probe Accu probe assay
that served as the reference method for detection of MTBC.
H37Rv reference strain was taken as a control for ICT test and
molecular tests. The reference strain showed the presence of
MPT64 antigen band in the ICT test. Similar bands were formed
in all MTBC (182) isolates tested, proving 100 per cent sensitivity
and no bands were detected in 48 (100%) NTM isolates tested,
proving 100 per cent specificity of the ICT kit.
Conclusion: Tuberculosis is a global pandemic. Rapid identification of Mycobacteria as MTB complex or non-tuberculous
Mycobacteria from culture is important for treatment of infected
cases and drug susceptibility testing of the culture isolate. MPT 64
TB antigen detection using SD Bioline Immunochromatographic
test is a simple and cost effective method for differentiation of
Mycobacterial cultures as MTB complex from non- tuberculous
Mycobacteria. |
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| ISSN: | 2249-782X 0973-709X |