Rapid Immunochromatographic Test for the Identification and Discrimination of Mycobacterium tuberculosis Complex Isolates from Non-tuberculous Mycobacteria
Background: A new rapid Immunochromatographic test (ICT) kit (SDBioline TB Ag MPT64RAPID®) developed by Standard Diagnostics, South Korea was evaluated for rapid differentiation of M. tuberculosis from non tuberculous mycobacteria (NTM). It detects MPT 64 antigen in M. tuberculosis isolates usin...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2014-04-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/4253/7098_E(C)_F(T)_PF1(PAK)_PFA(P)_PF2(PN).pdf |
| _version_ | 1818758251236294656 |
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| author | Vishnu Prasad Shenoy Chiranjay Mukhopadhyay |
| author_facet | Vishnu Prasad Shenoy Chiranjay Mukhopadhyay |
| author_sort | Vishnu Prasad Shenoy |
| collection | DOAJ |
| description | Background: A new rapid Immunochromatographic test (ICT)
kit (SDBioline TB Ag MPT64RAPID®) developed by Standard
Diagnostics, South Korea was evaluated for rapid differentiation
of M. tuberculosis from non tuberculous mycobacteria (NTM). It
detects MPT 64 antigen in M. tuberculosis isolates using mouse
monoclonal MPT 64 antibody. The kit was assessed for routine
identification of the Acid Fast Bacilli(AFB) isolated in our laboratory.
Materials and Methods: Two hundred eight culture isolates
of Mycobacteria were tested using ICT test kit for detection of
MPT 64 antigen from liquid and solid culture. H37Rv strain was
employed as the positive reference control. Any negative result
was referred for confirmation by Gen Probe Accu Probe assay
for MTB Complex (Gen-Probe, San Diego, Calif.). Speciation of
NTM was performed using genotypic Mycobacterium CM assay
(Hain’s life sciences, Germany).
Results: Of the 208 culture positive isolates tested, 182 (87.5%)
were found positive for Mycobacterium tuberculosis Complex
and remaining 26 (12.5%) were considered as NTM. These
results were further confirmed by Gen Probe Accu probe assay
that served as the reference method for detection of MTBC.
H37Rv reference strain was taken as a control for ICT test and
molecular tests. The reference strain showed the presence of
MPT64 antigen band in the ICT test. Similar bands were formed
in all MTBC (182) isolates tested, proving 100 per cent sensitivity
and no bands were detected in 48 (100%) NTM isolates tested,
proving 100 per cent specificity of the ICT kit.
Conclusion: Tuberculosis is a global pandemic. Rapid identification of Mycobacteria as MTB complex or non-tuberculous
Mycobacteria from culture is important for treatment of infected
cases and drug susceptibility testing of the culture isolate. MPT 64
TB antigen detection using SD Bioline Immunochromatographic
test is a simple and cost effective method for differentiation of
Mycobacterial cultures as MTB complex from non- tuberculous
Mycobacteria. |
| first_indexed | 2024-12-18T06:23:51Z |
| format | Article |
| id | doaj.art-20385a6d4d1f43479d11f459b231b2ab |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-18T06:23:51Z |
| publishDate | 2014-04-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-20385a6d4d1f43479d11f459b231b2ab2022-12-21T21:18:05ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-04-0184DC13DC1510.7860/JCDR/2014/7098.4253Rapid Immunochromatographic Test for the Identification and Discrimination of Mycobacterium tuberculosis Complex Isolates from Non-tuberculous MycobacteriaVishnu Prasad Shenoy0Chiranjay Mukhopadhyay1Associate professor Department of Microbiology, Kasturba Medical College Manipal, Karnataka, India.Professor and Head, Department of Microbiology, Kasturba Medical College Manipal, Karnataka, India.Background: A new rapid Immunochromatographic test (ICT) kit (SDBioline TB Ag MPT64RAPID®) developed by Standard Diagnostics, South Korea was evaluated for rapid differentiation of M. tuberculosis from non tuberculous mycobacteria (NTM). It detects MPT 64 antigen in M. tuberculosis isolates using mouse monoclonal MPT 64 antibody. The kit was assessed for routine identification of the Acid Fast Bacilli(AFB) isolated in our laboratory. Materials and Methods: Two hundred eight culture isolates of Mycobacteria were tested using ICT test kit for detection of MPT 64 antigen from liquid and solid culture. H37Rv strain was employed as the positive reference control. Any negative result was referred for confirmation by Gen Probe Accu Probe assay for MTB Complex (Gen-Probe, San Diego, Calif.). Speciation of NTM was performed using genotypic Mycobacterium CM assay (Hain’s life sciences, Germany). Results: Of the 208 culture positive isolates tested, 182 (87.5%) were found positive for Mycobacterium tuberculosis Complex and remaining 26 (12.5%) were considered as NTM. These results were further confirmed by Gen Probe Accu probe assay that served as the reference method for detection of MTBC. H37Rv reference strain was taken as a control for ICT test and molecular tests. The reference strain showed the presence of MPT64 antigen band in the ICT test. Similar bands were formed in all MTBC (182) isolates tested, proving 100 per cent sensitivity and no bands were detected in 48 (100%) NTM isolates tested, proving 100 per cent specificity of the ICT kit. Conclusion: Tuberculosis is a global pandemic. Rapid identification of Mycobacteria as MTB complex or non-tuberculous Mycobacteria from culture is important for treatment of infected cases and drug susceptibility testing of the culture isolate. MPT 64 TB antigen detection using SD Bioline Immunochromatographic test is a simple and cost effective method for differentiation of Mycobacterial cultures as MTB complex from non- tuberculous Mycobacteria.https://jcdr.net/articles/PDF/4253/7098_E(C)_F(T)_PF1(PAK)_PFA(P)_PF2(PN).pdfmpt 64 antigenm tuberculosis complexaccu probemycobacterium cm assay |
| spellingShingle | Vishnu Prasad Shenoy Chiranjay Mukhopadhyay Rapid Immunochromatographic Test for the Identification and Discrimination of Mycobacterium tuberculosis Complex Isolates from Non-tuberculous Mycobacteria Journal of Clinical and Diagnostic Research mpt 64 antigen m tuberculosis complex accu probe mycobacterium cm assay |
| title | Rapid Immunochromatographic Test for the Identification and Discrimination of Mycobacterium tuberculosis Complex Isolates from Non-tuberculous Mycobacteria |
| title_full | Rapid Immunochromatographic Test for the Identification and Discrimination of Mycobacterium tuberculosis Complex Isolates from Non-tuberculous Mycobacteria |
| title_fullStr | Rapid Immunochromatographic Test for the Identification and Discrimination of Mycobacterium tuberculosis Complex Isolates from Non-tuberculous Mycobacteria |
| title_full_unstemmed | Rapid Immunochromatographic Test for the Identification and Discrimination of Mycobacterium tuberculosis Complex Isolates from Non-tuberculous Mycobacteria |
| title_short | Rapid Immunochromatographic Test for the Identification and Discrimination of Mycobacterium tuberculosis Complex Isolates from Non-tuberculous Mycobacteria |
| title_sort | rapid immunochromatographic test for the identification and discrimination of mycobacterium tuberculosis complex isolates from non tuberculous mycobacteria |
| topic | mpt 64 antigen m tuberculosis complex accu probe mycobacterium cm assay |
| url | https://jcdr.net/articles/PDF/4253/7098_E(C)_F(T)_PF1(PAK)_PFA(P)_PF2(PN).pdf |
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