Validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich Elisa
The paper describes the process of the validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich ELISA for FMD types A, 0 and Asial. The results were analyzed and the following main validation characteristics were determined: sensitivity, specificity,...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Da Vinci Media
2018-04-01
|
| Series: | Ветеринария сегодня |
| Subjects: | |
| Online Access: | https://veterinary.arriah.ru/jour/article/view/203 |
| _version_ | 1826812514274377728 |
|---|---|
| author | N. N. Lugovskaya Ye. N. Kalinina K. S. Maikova O. V. Vorobyova G. M. Goryacheva T. K. Mayorova |
| author_facet | N. N. Lugovskaya Ye. N. Kalinina K. S. Maikova O. V. Vorobyova G. M. Goryacheva T. K. Mayorova |
| author_sort | N. N. Lugovskaya |
| collection | DOAJ |
| description | The paper describes the process of the validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich ELISA for FMD types A, 0 and Asial. The results were analyzed and the following main validation characteristics were determined: sensitivity, specificity, accuracy, consistency, predictability of positive and negative results, reproducibility and intermediate precision under the conditions of repeatability and reproducibility. The results of the ELISA procedure validation were in compliance with the acceptability criteria. |
| first_indexed | 2024-03-09T02:49:59Z |
| format | Article |
| id | doaj.art-203bc7b4748a4ebdaf79397863efc4b1 |
| institution | Directory Open Access Journal |
| issn | 2304-196X 2658-6959 |
| language | English |
| last_indexed | 2025-02-16T03:59:38Z |
| publishDate | 2018-04-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj.art-203bc7b4748a4ebdaf79397863efc4b12025-02-06T09:52:05ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-04-01032229203Validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich ElisaN. N. Lugovskaya0Ye. N. Kalinina1K. S. Maikova2O. V. Vorobyova3G. M. Goryacheva4T. K. Mayorova5ФГБУ «ВНИИЗЖ», г. ВладимирФГБУ «ВНИИЗЖ», г. ВладимирФГБУ «ВНИИЗЖ», г. ВладимирФГБУ «ВНИИЗЖ», г. ВладимирФГБУ «ВНИИЗЖ», г. ВладимирФГБУ «ВНИИЗЖ», г. ВладимирThe paper describes the process of the validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich ELISA for FMD types A, 0 and Asial. The results were analyzed and the following main validation characteristics were determined: sensitivity, specificity, accuracy, consistency, predictability of positive and negative results, reproducibility and intermediate precision under the conditions of repeatability and reproducibility. The results of the ELISA procedure validation were in compliance with the acceptability criteria.https://veterinary.arriah.ru/jour/article/view/203валидацияиммуноферментный анализантителаящурvalidationelisaantibodiesfmd |
| spellingShingle | N. N. Lugovskaya Ye. N. Kalinina K. S. Maikova O. V. Vorobyova G. M. Goryacheva T. K. Mayorova Validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich Elisa Ветеринария сегодня валидация иммуноферментный анализ антитела ящур validation elisa antibodies fmd |
| title | Validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich Elisa |
| title_full | Validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich Elisa |
| title_fullStr | Validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich Elisa |
| title_full_unstemmed | Validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich Elisa |
| title_short | Validation of the technique aimed at the determination of FMD antibodies in liquid phase blocking sandwich Elisa |
| title_sort | validation of the technique aimed at the determination of fmd antibodies in liquid phase blocking sandwich elisa |
| topic | валидация иммуноферментный анализ антитела ящур validation elisa antibodies fmd |
| url | https://veterinary.arriah.ru/jour/article/view/203 |
| work_keys_str_mv | AT nnlugovskaya validationofthetechniqueaimedatthedeterminationoffmdantibodiesinliquidphaseblockingsandwichelisa AT yenkalinina validationofthetechniqueaimedatthedeterminationoffmdantibodiesinliquidphaseblockingsandwichelisa AT ksmaikova validationofthetechniqueaimedatthedeterminationoffmdantibodiesinliquidphaseblockingsandwichelisa AT ovvorobyova validationofthetechniqueaimedatthedeterminationoffmdantibodiesinliquidphaseblockingsandwichelisa AT gmgoryacheva validationofthetechniqueaimedatthedeterminationoffmdantibodiesinliquidphaseblockingsandwichelisa AT tkmayorova validationofthetechniqueaimedatthedeterminationoffmdantibodiesinliquidphaseblockingsandwichelisa |