Effect of Centella asiatica (L.) Urb. extracts through expression of SIRT1 and Per2 on zebrafish (Danio rerio) larvae insomnia model
Context: Insomnia is difficulty falling asleep or maintaining sleep or sleeping conditions that cannot adequately restore the body and mental state. However, insomnia therapy has not been satisfying for improving the condition. Several alternative therapy options for treating insomnia include Centel...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
GarVal Editorial Ltda.
2024-03-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | https://jppres.com/jppres/pdf/vol12/jppres23.1737_12.2.286.pdf |
| _version_ | 1797356183316594688 |
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| author | Zamroni Afif Nurvia Andriani Rodhiyan Rakhmatiar Shahdevi Kurniawan Holipah Husnul Khotimah Nurdiana Irawan Satriotomo |
| author_facet | Zamroni Afif Nurvia Andriani Rodhiyan Rakhmatiar Shahdevi Kurniawan Holipah Husnul Khotimah Nurdiana Irawan Satriotomo |
| author_sort | Zamroni Afif |
| collection | DOAJ |
| description | Context: Insomnia is difficulty falling asleep or maintaining sleep or sleeping conditions that cannot adequately restore the body and mental state. However, insomnia therapy has not been satisfying for improving the condition. Several alternative therapy options for treating insomnia include Centella asiatica (CA). Many pathways lead to sleep regulation, including circadian rhythms and cellular reactions. Further research is necessary to treat insomnia through circadian rhythms by administering CA with observations on zebrafish models of insomnia.
Aims: To evaluate the influence of CA extract administration on deacetylase sirtuin-1 (SIRT1) and period circadian regulator-2 (Per2) expression in zebrafish (Danio rerio) larvae in an insomnia model.
Methods: A laboratory experimental study with a randomized control group post-test only was used, with a sample of D. rerio larvae insomnia model that was given treatment with CA ethanolic extract (2.5, 5, and 10 µg/mL). The D. rerio larvae were prepared for reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to evaluate SIRT1 and Per2 levels.
Results: This study found a significant effect of CA ethanolic extract on the expression of SIRT1 (p=0.001), with CA extract 10 µg/mL elevating SIRT1 expression in the group of D. rerio larvae with the insomnia model. There was no significant effect of CA extract on Per2 expression in the group of D. rerio larvae with the insomnia model (p=0.051).
Conclusions: Administration of CA extract at a concentration of 10 µg/mL can elevate the expression of SIRT1 and have no significant effect on Per2 expression in D. rerio larvae with insomnia model. |
| first_indexed | 2024-03-08T14:21:54Z |
| format | Article |
| id | doaj.art-203edaccd4944a0f91e7c7c73ca59237 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-03-08T14:21:54Z |
| publishDate | 2024-03-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-203edaccd4944a0f91e7c7c73ca592372024-01-14T04:32:02ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502024-03-0112228629510.56499/jppres23.1737_12.2.286Effect of Centella asiatica (L.) Urb. extracts through expression of SIRT1 and Per2 on zebrafish (Danio rerio) larvae insomnia modelZamroni Afif0Nurvia Andriani1Rodhiyan Rakhmatiar2Shahdevi Kurniawan3Holipah4Husnul Khotimah5Nurdiana6Irawan Satriotomo7Doctoral Program in Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia. Department of Neurology Faculty of Medicine, Universitas Brawijaya, Saiful Anwar General Hospital, Malang, Indonesia.Department of Neurology Faculty of Medicine, Universitas Brawijaya, Saiful Anwar General Hospital, Malang, Indonesia.Department of Neurology Faculty of Medicine, Universitas Brawijaya, Saiful Anwar General Hospital, Malang, Indonesia.Department of Neurology Faculty of Medicine, Universitas Brawijaya, Saiful Anwar General Hospital, Malang, Indonesia.Department of Public Health, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.Departement of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.Departement of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.Indonesian Neuroscience Institute, Malang, Indonesia.Context: Insomnia is difficulty falling asleep or maintaining sleep or sleeping conditions that cannot adequately restore the body and mental state. However, insomnia therapy has not been satisfying for improving the condition. Several alternative therapy options for treating insomnia include Centella asiatica (CA). Many pathways lead to sleep regulation, including circadian rhythms and cellular reactions. Further research is necessary to treat insomnia through circadian rhythms by administering CA with observations on zebrafish models of insomnia. Aims: To evaluate the influence of CA extract administration on deacetylase sirtuin-1 (SIRT1) and period circadian regulator-2 (Per2) expression in zebrafish (Danio rerio) larvae in an insomnia model. Methods: A laboratory experimental study with a randomized control group post-test only was used, with a sample of D. rerio larvae insomnia model that was given treatment with CA ethanolic extract (2.5, 5, and 10 µg/mL). The D. rerio larvae were prepared for reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to evaluate SIRT1 and Per2 levels. Results: This study found a significant effect of CA ethanolic extract on the expression of SIRT1 (p=0.001), with CA extract 10 µg/mL elevating SIRT1 expression in the group of D. rerio larvae with the insomnia model. There was no significant effect of CA extract on Per2 expression in the group of D. rerio larvae with the insomnia model (p=0.051). Conclusions: Administration of CA extract at a concentration of 10 µg/mL can elevate the expression of SIRT1 and have no significant effect on Per2 expression in D. rerio larvae with insomnia model.https://jppres.com/jppres/pdf/vol12/jppres23.1737_12.2.286.pdfcentella asiaticacircadian rhythmdeacetylase sirtuin-1insomniaperiod circadian regulator-2zebrafish |
| spellingShingle | Zamroni Afif Nurvia Andriani Rodhiyan Rakhmatiar Shahdevi Kurniawan Holipah Husnul Khotimah Nurdiana Irawan Satriotomo Effect of Centella asiatica (L.) Urb. extracts through expression of SIRT1 and Per2 on zebrafish (Danio rerio) larvae insomnia model Journal of Pharmacy & Pharmacognosy Research centella asiatica circadian rhythm deacetylase sirtuin-1 insomnia period circadian regulator-2 zebrafish |
| title | Effect of Centella asiatica (L.) Urb. extracts through expression of SIRT1 and Per2 on zebrafish (Danio rerio) larvae insomnia model |
| title_full | Effect of Centella asiatica (L.) Urb. extracts through expression of SIRT1 and Per2 on zebrafish (Danio rerio) larvae insomnia model |
| title_fullStr | Effect of Centella asiatica (L.) Urb. extracts through expression of SIRT1 and Per2 on zebrafish (Danio rerio) larvae insomnia model |
| title_full_unstemmed | Effect of Centella asiatica (L.) Urb. extracts through expression of SIRT1 and Per2 on zebrafish (Danio rerio) larvae insomnia model |
| title_short | Effect of Centella asiatica (L.) Urb. extracts through expression of SIRT1 and Per2 on zebrafish (Danio rerio) larvae insomnia model |
| title_sort | effect of centella asiatica l urb extracts through expression of sirt1 and per2 on zebrafish danio rerio larvae insomnia model |
| topic | centella asiatica circadian rhythm deacetylase sirtuin-1 insomnia period circadian regulator-2 zebrafish |
| url | https://jppres.com/jppres/pdf/vol12/jppres23.1737_12.2.286.pdf |
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