Phylogenetic and genomic analyses of two new species of Clavispora (Metschnikowiaceae, Saccharomycetales) from Central China
Species in the genus Clavispora have previously been reported primarily in the northeast and northwest regions of China; the species diversity of Clavispora in central China is not currently clear. In this study, phylogenetic inferences of Clavispora based on sequences of a single-locus (LSU D1/D2)...
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1019599/full |
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| author | Chun-Yue Chai Chun-Yue Chai Ying Li Zhen-Li Yan Feng-Li Hui Feng-Li Hui |
| author_facet | Chun-Yue Chai Chun-Yue Chai Ying Li Zhen-Li Yan Feng-Li Hui Feng-Li Hui |
| author_sort | Chun-Yue Chai |
| collection | DOAJ |
| description | Species in the genus Clavispora have previously been reported primarily in the northeast and northwest regions of China; the species diversity of Clavispora in central China is not currently clear. In this study, phylogenetic inferences of Clavispora based on sequences of a single-locus (LSU D1/D2) and a two-locus (LSU D1/D2 and ITS) were conducted. Two new species isolated from rotting wood in central China, namely Clavispora xylosa sp. nov. and Clavispora paralusitaniae sp. nov., were delimited and proposed based on morphological and molecular evidence. Cl. xylosa was closely related to C. thailandica CBS 10610T, but with 11.5% divergence in the LSU D1/D2 domains and 11.5% divergence in the ITS regions. Cl. paralusitaniae was a sister to Cl. lusitaniae CBS 6936T from which it differs with 4.7% divergence in the LSU D1/D2 domains and 5.4% divergence in the ITS regions. Description of Cl. xylosa sp. nov. and Cl. paralusitaniae sp. nov. was also supported by morphological comparisons and genomic analyses between the two new species and their closest relatives, C. thailandica CBS 10610T and Cl. lusitaniae CBS 6936T. These results indicate a potentially great diversity of Clavispora spp. inhabiting rotting wood in central China, ripe for future discovery. |
| first_indexed | 2024-04-11T07:15:13Z |
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| id | doaj.art-20417a910d56453d9580e0696f5b01d1 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T07:15:13Z |
| publishDate | 2022-10-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-20417a910d56453d9580e0696f5b01d12022-12-22T04:37:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10195991019599Phylogenetic and genomic analyses of two new species of Clavispora (Metschnikowiaceae, Saccharomycetales) from Central ChinaChun-Yue Chai0Chun-Yue Chai1Ying Li2Zhen-Li Yan3Feng-Li Hui4Feng-Li Hui5College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, ChinaResearch Center of Henan Provincial Agricultural Biomass Resource Engineering and Technology, Nanyang Normal University, Nanyang, ChinaCollege of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, ChinaState Key Laboratory of Motor Vehicle Biofuel Technology, Henan Tianguan Enterprise Group Co., Ltd, Nanyang, ChinaCollege of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, ChinaResearch Center of Henan Provincial Agricultural Biomass Resource Engineering and Technology, Nanyang Normal University, Nanyang, ChinaSpecies in the genus Clavispora have previously been reported primarily in the northeast and northwest regions of China; the species diversity of Clavispora in central China is not currently clear. In this study, phylogenetic inferences of Clavispora based on sequences of a single-locus (LSU D1/D2) and a two-locus (LSU D1/D2 and ITS) were conducted. Two new species isolated from rotting wood in central China, namely Clavispora xylosa sp. nov. and Clavispora paralusitaniae sp. nov., were delimited and proposed based on morphological and molecular evidence. Cl. xylosa was closely related to C. thailandica CBS 10610T, but with 11.5% divergence in the LSU D1/D2 domains and 11.5% divergence in the ITS regions. Cl. paralusitaniae was a sister to Cl. lusitaniae CBS 6936T from which it differs with 4.7% divergence in the LSU D1/D2 domains and 5.4% divergence in the ITS regions. Description of Cl. xylosa sp. nov. and Cl. paralusitaniae sp. nov. was also supported by morphological comparisons and genomic analyses between the two new species and their closest relatives, C. thailandica CBS 10610T and Cl. lusitaniae CBS 6936T. These results indicate a potentially great diversity of Clavispora spp. inhabiting rotting wood in central China, ripe for future discovery.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1019599/fullClavispora paralusitaniae sp. nov.taxonomyrotted wood-inhabiting yeastgenomic analysesphylogenyClavispora xylosa sp. nov. |
| spellingShingle | Chun-Yue Chai Chun-Yue Chai Ying Li Zhen-Li Yan Feng-Li Hui Feng-Li Hui Phylogenetic and genomic analyses of two new species of Clavispora (Metschnikowiaceae, Saccharomycetales) from Central China Frontiers in Microbiology Clavispora paralusitaniae sp. nov. taxonomy rotted wood-inhabiting yeast genomic analyses phylogeny Clavispora xylosa sp. nov. |
| title | Phylogenetic and genomic analyses of two new species of Clavispora (Metschnikowiaceae, Saccharomycetales) from Central China |
| title_full | Phylogenetic and genomic analyses of two new species of Clavispora (Metschnikowiaceae, Saccharomycetales) from Central China |
| title_fullStr | Phylogenetic and genomic analyses of two new species of Clavispora (Metschnikowiaceae, Saccharomycetales) from Central China |
| title_full_unstemmed | Phylogenetic and genomic analyses of two new species of Clavispora (Metschnikowiaceae, Saccharomycetales) from Central China |
| title_short | Phylogenetic and genomic analyses of two new species of Clavispora (Metschnikowiaceae, Saccharomycetales) from Central China |
| title_sort | phylogenetic and genomic analyses of two new species of clavispora metschnikowiaceae saccharomycetales from central china |
| topic | Clavispora paralusitaniae sp. nov. taxonomy rotted wood-inhabiting yeast genomic analyses phylogeny Clavispora xylosa sp. nov. |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1019599/full |
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