Oxygen tension and oocyte density during in vitro maturation affect the in vitro fertilization of bovine oocytes
Oocyte maturation is the key factor affecting the fertilization and embryonic development. Factors such as oocyte density and oxygen tension can directly influence the IMV. Thus, the objective of this study was to evaluate the effect of the association of oxygen tensions (5% or 20%) with different...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual de Londrina
2015-12-01
|
| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19990 |
| _version_ | 1797944256541753344 |
|---|---|
| author | Angelo Bertani Giotto Daniela dos Santos Brum Francielli Weber Santos Antonio Carlos Galarça Guimarães Cibele Garcia Moreira Gonçalves Cecilia Urquiza Machado Pavin Natalia Picoli Folchini Aline Barros Moyses Daniele Missio Fábio Gallas Leivas |
| author_facet | Angelo Bertani Giotto Daniela dos Santos Brum Francielli Weber Santos Antonio Carlos Galarça Guimarães Cibele Garcia Moreira Gonçalves Cecilia Urquiza Machado Pavin Natalia Picoli Folchini Aline Barros Moyses Daniele Missio Fábio Gallas Leivas |
| author_sort | Angelo Bertani Giotto |
| collection | DOAJ |
| description |
Oocyte maturation is the key factor affecting the fertilization and embryonic development. Factors such as oocyte density and oxygen tension can directly influence the IMV. Thus, the objective of this study was to evaluate the effect of the association of oxygen tensions (5% or 20%) with different oocyte densities (1:10?l or 1:20?l) in the in vitro maturation (IVM) of bovine oocytes on maturation and fertilization rates, ROS production and antioxidant activity. Three experiments were performed with bovine oocytes that were obtained from slaughterhouse ovaries. After selection, the oocytes were randomly distributed in four treatments: 1:10/5%; 1:10/20%; 1:20/5%and 1:20/20% for each experiment. In experiment I, nuclear maturation status and cytoplasmic maturation were evaluated through detection of the first polar body by immunofluorescence and the mitochondrial reorganization assay. In experiment II, ROS production and antioxidant activity were analyzed in oocytes and IVM medium after 24 h of maturation through detection of ROS, reduced glutathione (GSH) and Superoxide dismutase activity by spectrofluorimetric methods. In experiment III, fertilization was evaluated through pronucleus formation, sperm penetration with or without decondensation and polyspermy rates by immunofluorescence. In experiment I, the nuclear maturation and cytoplasmic maturation were similar among treatments (P>0.05). In experiment II, reactive oxygen species in oocytes were elevated in treatments with low oxygen tension which was independent of oocyte density (P<0.05). Additionally, ROS levels in IVM medium were higher in treatments with high oocyte density by volume of medium, which was independent of oxygen tension (P<0.05). In Experiment III, the fertilization and penetration rates were higher in the treatment with 20% oxygen tension and high oocyte density (P<0.05). Furthermore, a high incidence of polyspermy was observed in groups with high oxygen tension and low oocyte density (P<0.05). In conclusion, the results of this study indicate an interaction between oxygen tension and oocyte density, which increases ROS production in certain associations and subsequently influences the rates of in vitro fertilization of bovine oocytes. The improved rates of IVF were obtained when IVM was conducted using 20% oxygen tension and high oocyte density (1:20 ul).
|
| first_indexed | 2024-04-10T20:35:45Z |
| format | Article |
| id | doaj.art-2044bc53831946598fd9c101e9d033fd |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-04-10T20:35:45Z |
| publishDate | 2015-12-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-2044bc53831946598fd9c101e9d033fd2023-01-24T20:38:19ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592015-12-01366Supl210.5433/1679-0359.2015v36n6Supl2p4277Oxygen tension and oocyte density during in vitro maturation affect the in vitro fertilization of bovine oocytesAngelo Bertani Giotto0Daniela dos Santos Brum1Francielli Weber Santos2Antonio Carlos Galarça Guimarães3Cibele Garcia Moreira Gonçalves4Cecilia Urquiza Machado Pavin5Natalia Picoli Folchini6Aline Barros Moyses7Daniele Missio8Fábio Gallas Leivas9Universidade Federal do PampaUniversidade Federal do PampaUniversidade Federal do PampaUniversidade Federal do PampaUniversidade Federal do PampaUniversidade Federal do PampaUniversidade Federal do PampaUniversidade Federal do PampaUniversidade Federal do PampaUniversidade Federal do Pampa Oocyte maturation is the key factor affecting the fertilization and embryonic development. Factors such as oocyte density and oxygen tension can directly influence the IMV. Thus, the objective of this study was to evaluate the effect of the association of oxygen tensions (5% or 20%) with different oocyte densities (1:10?l or 1:20?l) in the in vitro maturation (IVM) of bovine oocytes on maturation and fertilization rates, ROS production and antioxidant activity. Three experiments were performed with bovine oocytes that were obtained from slaughterhouse ovaries. After selection, the oocytes were randomly distributed in four treatments: 1:10/5%; 1:10/20%; 1:20/5%and 1:20/20% for each experiment. In experiment I, nuclear maturation status and cytoplasmic maturation were evaluated through detection of the first polar body by immunofluorescence and the mitochondrial reorganization assay. In experiment II, ROS production and antioxidant activity were analyzed in oocytes and IVM medium after 24 h of maturation through detection of ROS, reduced glutathione (GSH) and Superoxide dismutase activity by spectrofluorimetric methods. In experiment III, fertilization was evaluated through pronucleus formation, sperm penetration with or without decondensation and polyspermy rates by immunofluorescence. In experiment I, the nuclear maturation and cytoplasmic maturation were similar among treatments (P>0.05). In experiment II, reactive oxygen species in oocytes were elevated in treatments with low oxygen tension which was independent of oocyte density (P<0.05). Additionally, ROS levels in IVM medium were higher in treatments with high oocyte density by volume of medium, which was independent of oxygen tension (P<0.05). In Experiment III, the fertilization and penetration rates were higher in the treatment with 20% oxygen tension and high oocyte density (P<0.05). Furthermore, a high incidence of polyspermy was observed in groups with high oxygen tension and low oocyte density (P<0.05). In conclusion, the results of this study indicate an interaction between oxygen tension and oocyte density, which increases ROS production in certain associations and subsequently influences the rates of in vitro fertilization of bovine oocytes. The improved rates of IVF were obtained when IVM was conducted using 20% oxygen tension and high oocyte density (1:20 ul). https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19990Oocyte densityOxygen tensionROS productionSuperoxide dismutaseGSH. |
| spellingShingle | Angelo Bertani Giotto Daniela dos Santos Brum Francielli Weber Santos Antonio Carlos Galarça Guimarães Cibele Garcia Moreira Gonçalves Cecilia Urquiza Machado Pavin Natalia Picoli Folchini Aline Barros Moyses Daniele Missio Fábio Gallas Leivas Oxygen tension and oocyte density during in vitro maturation affect the in vitro fertilization of bovine oocytes Semina: Ciências Agrárias Oocyte density Oxygen tension ROS production Superoxide dismutase GSH. |
| title | Oxygen tension and oocyte density during in vitro maturation affect the in vitro fertilization of bovine oocytes |
| title_full | Oxygen tension and oocyte density during in vitro maturation affect the in vitro fertilization of bovine oocytes |
| title_fullStr | Oxygen tension and oocyte density during in vitro maturation affect the in vitro fertilization of bovine oocytes |
| title_full_unstemmed | Oxygen tension and oocyte density during in vitro maturation affect the in vitro fertilization of bovine oocytes |
| title_short | Oxygen tension and oocyte density during in vitro maturation affect the in vitro fertilization of bovine oocytes |
| title_sort | oxygen tension and oocyte density during in vitro maturation affect the in vitro fertilization of bovine oocytes |
| topic | Oocyte density Oxygen tension ROS production Superoxide dismutase GSH. |
| url | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/19990 |
| work_keys_str_mv | AT angelobertanigiotto oxygentensionandoocytedensityduringinvitromaturationaffecttheinvitrofertilizationofbovineoocytes AT danieladossantosbrum oxygentensionandoocytedensityduringinvitromaturationaffecttheinvitrofertilizationofbovineoocytes AT francielliwebersantos oxygentensionandoocytedensityduringinvitromaturationaffecttheinvitrofertilizationofbovineoocytes AT antoniocarlosgalarcaguimaraes oxygentensionandoocytedensityduringinvitromaturationaffecttheinvitrofertilizationofbovineoocytes AT cibelegarciamoreiragoncalves oxygentensionandoocytedensityduringinvitromaturationaffecttheinvitrofertilizationofbovineoocytes AT ceciliaurquizamachadopavin oxygentensionandoocytedensityduringinvitromaturationaffecttheinvitrofertilizationofbovineoocytes AT nataliapicolifolchini oxygentensionandoocytedensityduringinvitromaturationaffecttheinvitrofertilizationofbovineoocytes AT alinebarrosmoyses oxygentensionandoocytedensityduringinvitromaturationaffecttheinvitrofertilizationofbovineoocytes AT danielemissio oxygentensionandoocytedensityduringinvitromaturationaffecttheinvitrofertilizationofbovineoocytes AT fabiogallasleivas oxygentensionandoocytedensityduringinvitromaturationaffecttheinvitrofertilizationofbovineoocytes |