Effect and mechanism of Tangzhiqing in improving cardiac function in mice with hyperlipidaemia complicated with myocardial ischaemia
Purpose: Tangzhiqing formula (TZQ) is a traditional Chinese medicine prescribed to treat lipid metabolism disorders, atherosclerosis, diabetes and diabetic cardiomyopathy. However, some challenges and hurdles remain. TZQ showed promising results in treating diabetes and hyperlipidaemia. However, its...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023028529 |
| _version_ | 1827937431704305664 |
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| author | Zhihui Song Rui Chen Caijun Wang Guiyun Pan An Yan Guinan Xie Zhihua Yang Wanying Feng Yi Wang |
| author_facet | Zhihui Song Rui Chen Caijun Wang Guiyun Pan An Yan Guinan Xie Zhihua Yang Wanying Feng Yi Wang |
| author_sort | Zhihui Song |
| collection | DOAJ |
| description | Purpose: Tangzhiqing formula (TZQ) is a traditional Chinese medicine prescribed to treat lipid metabolism disorders, atherosclerosis, diabetes and diabetic cardiomyopathy. However, some challenges and hurdles remain. TZQ showed promising results in treating diabetes and hyperlipidaemia. However, its effect on and mechanism of action in hyperlipidaemia complicated with myocardial ischaemia (HL-MI) remain unknown. Methods: In this study, a network pharmacology-based strategy integrating target prediction was adopted to predict the targets of TZQ relevant to the treatment of HL-MI and to further explore the involved pharmacological mechanisms. Results: A total of 104 potential therapeutic targets were obtained, including MMP9, Bcl-2, and Bax, which may be related to the apoptosis and PI3K/AKT signalling pathways. Then, we confirmed these potential targets and pathways with animal experimentation. TZQ reduced lipid levels, increased the expression levels of Bcl-2, decreased Bax, caspase-3 and caspase-9 expression levels, and activated the PI3K/AKT signalling pathway. Conclusion: In conclusion, this study provides new insights into the protective mechanisms of TZQ against HL-MI through network pharmacology and pharmacological approaches. |
| first_indexed | 2024-03-13T08:25:32Z |
| format | Article |
| id | doaj.art-20466528cf9249449cd7f3ffe00bc472 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T08:25:32Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-20466528cf9249449cd7f3ffe00bc4722023-05-31T04:45:02ZengElsevierHeliyon2405-84402023-05-0195e15645Effect and mechanism of Tangzhiqing in improving cardiac function in mice with hyperlipidaemia complicated with myocardial ischaemiaZhihui Song0Rui Chen1Caijun Wang2Guiyun Pan3An Yan4Guinan Xie5Zhihua Yang6Wanying Feng7Yi Wang8Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaCollege of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China; Corresponding author. Tianjin University of Traditional Chinese Medicine, #10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin, 301617, China.Purpose: Tangzhiqing formula (TZQ) is a traditional Chinese medicine prescribed to treat lipid metabolism disorders, atherosclerosis, diabetes and diabetic cardiomyopathy. However, some challenges and hurdles remain. TZQ showed promising results in treating diabetes and hyperlipidaemia. However, its effect on and mechanism of action in hyperlipidaemia complicated with myocardial ischaemia (HL-MI) remain unknown. Methods: In this study, a network pharmacology-based strategy integrating target prediction was adopted to predict the targets of TZQ relevant to the treatment of HL-MI and to further explore the involved pharmacological mechanisms. Results: A total of 104 potential therapeutic targets were obtained, including MMP9, Bcl-2, and Bax, which may be related to the apoptosis and PI3K/AKT signalling pathways. Then, we confirmed these potential targets and pathways with animal experimentation. TZQ reduced lipid levels, increased the expression levels of Bcl-2, decreased Bax, caspase-3 and caspase-9 expression levels, and activated the PI3K/AKT signalling pathway. Conclusion: In conclusion, this study provides new insights into the protective mechanisms of TZQ against HL-MI through network pharmacology and pharmacological approaches.http://www.sciencedirect.com/science/article/pii/S2405844023028529TangzhiqingNetwork analysisHyperlipidaemiaMyocardial ischaemiaPI3K/AKTApoptosis |
| spellingShingle | Zhihui Song Rui Chen Caijun Wang Guiyun Pan An Yan Guinan Xie Zhihua Yang Wanying Feng Yi Wang Effect and mechanism of Tangzhiqing in improving cardiac function in mice with hyperlipidaemia complicated with myocardial ischaemia Heliyon Tangzhiqing Network analysis Hyperlipidaemia Myocardial ischaemia PI3K/AKT Apoptosis |
| title | Effect and mechanism of Tangzhiqing in improving cardiac function in mice with hyperlipidaemia complicated with myocardial ischaemia |
| title_full | Effect and mechanism of Tangzhiqing in improving cardiac function in mice with hyperlipidaemia complicated with myocardial ischaemia |
| title_fullStr | Effect and mechanism of Tangzhiqing in improving cardiac function in mice with hyperlipidaemia complicated with myocardial ischaemia |
| title_full_unstemmed | Effect and mechanism of Tangzhiqing in improving cardiac function in mice with hyperlipidaemia complicated with myocardial ischaemia |
| title_short | Effect and mechanism of Tangzhiqing in improving cardiac function in mice with hyperlipidaemia complicated with myocardial ischaemia |
| title_sort | effect and mechanism of tangzhiqing in improving cardiac function in mice with hyperlipidaemia complicated with myocardial ischaemia |
| topic | Tangzhiqing Network analysis Hyperlipidaemia Myocardial ischaemia PI3K/AKT Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023028529 |
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