Distribution of the 23-bp polymorphism of the prion protein gene in Jersey cattles in Turkey
Background: Bovine spongiform encephalopathy (BSE) is a prion disease that is always fatal in cattle and is considered an important risk factor for human health. Genetic polymorphisms that alter prion proteins may be associated with susceptibility or resistance to infectious spongiform encephalopat...
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| Format: | Article |
| Language: | English |
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"Alexandru Ioan Cuza" University of Iași
2023-06-01
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| Series: | Journal of Experimental and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.jemb.bio.uaic.ro/index.php/jemb/article/view/91 |
| _version_ | 1797813921149616128 |
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| author | Melih Sercan USTAOGLU Serbulent YIGIT Muhammed Taha Kaya |
| author_facet | Melih Sercan USTAOGLU Serbulent YIGIT Muhammed Taha Kaya |
| author_sort | Melih Sercan USTAOGLU |
| collection | DOAJ |
| description |
Background: Bovine spongiform encephalopathy (BSE) is a prion disease that is always fatal in cattle and is considered an important risk factor for human health. Genetic polymorphisms that alter prion proteins may be associated with susceptibility or resistance to infectious spongiform encephalopathy. Therefore, we investigated the distribution of the 23 bp indel variant in the prion protein (PRNP) gene in Jersey cattle in Turkey.
Methods: A total of 95 unrelated Jersey cattle (79 of reproductive age and 16 of non-reproductive age) from a private farm in Izmir were included in the study. Genomic DNA was obtained from the milk of reproductive-age cattle and the saliva of non-reproductive-age cattle. A 23-bp indel polymorphism in the PRNP gene promoter region was genotyped by polymerase chain reaction (PCR) analysis.
Results: The three genotypes of the PRNP 23-bp indel variant were classified as follows: I/I (465 bp), I/D (306 bp) and D/D (both 465 and 306 bp fragments). The frequencies of the I/I, I/D, and D/D genotypes of the PRNP 23-bp indel variant in cattle were 22 (23.16%), 48 (50.53%), and 25 (26.32%). We then examined genotype and allele distribution according to service period. No significant difference was detected in terms of PRNP gene 23 bp-indel variant genotype and allele distribution in the groups created according to the service period (p>0.05).
Conclusion: Although the PRNP gene 23 bp-indel variant genotype and allele distribution in jersey-type cattle in Turkey did not differ according to service period, our results may benefit the understanding of the genetic characteristics of the PRNP gene in cattle breeds.
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| first_indexed | 2024-03-13T07:59:49Z |
| format | Article |
| id | doaj.art-20494d4db85a48b9a4a2de6e2ef925b4 |
| institution | Directory Open Access Journal |
| issn | 2601-6974 |
| language | English |
| last_indexed | 2024-03-13T07:59:49Z |
| publishDate | 2023-06-01 |
| publisher | "Alexandru Ioan Cuza" University of Iași |
| record_format | Article |
| series | Journal of Experimental and Molecular Biology |
| spelling | doaj.art-20494d4db85a48b9a4a2de6e2ef925b42023-06-01T21:16:30Zeng"Alexandru Ioan Cuza" University of IașiJournal of Experimental and Molecular Biology2601-69742023-06-0110.47743/jemb-2023-91Distribution of the 23-bp polymorphism of the prion protein gene in Jersey cattles in TurkeyMelih Sercan USTAOGLU0Serbulent YIGIT1Muhammed Taha Kaya2Amasya University Ondokuz Mayis UniversityOndokuz Mayis University Background: Bovine spongiform encephalopathy (BSE) is a prion disease that is always fatal in cattle and is considered an important risk factor for human health. Genetic polymorphisms that alter prion proteins may be associated with susceptibility or resistance to infectious spongiform encephalopathy. Therefore, we investigated the distribution of the 23 bp indel variant in the prion protein (PRNP) gene in Jersey cattle in Turkey. Methods: A total of 95 unrelated Jersey cattle (79 of reproductive age and 16 of non-reproductive age) from a private farm in Izmir were included in the study. Genomic DNA was obtained from the milk of reproductive-age cattle and the saliva of non-reproductive-age cattle. A 23-bp indel polymorphism in the PRNP gene promoter region was genotyped by polymerase chain reaction (PCR) analysis. Results: The three genotypes of the PRNP 23-bp indel variant were classified as follows: I/I (465 bp), I/D (306 bp) and D/D (both 465 and 306 bp fragments). The frequencies of the I/I, I/D, and D/D genotypes of the PRNP 23-bp indel variant in cattle were 22 (23.16%), 48 (50.53%), and 25 (26.32%). We then examined genotype and allele distribution according to service period. No significant difference was detected in terms of PRNP gene 23 bp-indel variant genotype and allele distribution in the groups created according to the service period (p>0.05). Conclusion: Although the PRNP gene 23 bp-indel variant genotype and allele distribution in jersey-type cattle in Turkey did not differ according to service period, our results may benefit the understanding of the genetic characteristics of the PRNP gene in cattle breeds. http://www.jemb.bio.uaic.ro/index.php/jemb/article/view/91Bovine spongiform encephalopathyprion proteinvariantPCR |
| spellingShingle | Melih Sercan USTAOGLU Serbulent YIGIT Muhammed Taha Kaya Distribution of the 23-bp polymorphism of the prion protein gene in Jersey cattles in Turkey Journal of Experimental and Molecular Biology Bovine spongiform encephalopathy prion protein variant PCR |
| title | Distribution of the 23-bp polymorphism of the prion protein gene in Jersey cattles in Turkey |
| title_full | Distribution of the 23-bp polymorphism of the prion protein gene in Jersey cattles in Turkey |
| title_fullStr | Distribution of the 23-bp polymorphism of the prion protein gene in Jersey cattles in Turkey |
| title_full_unstemmed | Distribution of the 23-bp polymorphism of the prion protein gene in Jersey cattles in Turkey |
| title_short | Distribution of the 23-bp polymorphism of the prion protein gene in Jersey cattles in Turkey |
| title_sort | distribution of the 23 bp polymorphism of the prion protein gene in jersey cattles in turkey |
| topic | Bovine spongiform encephalopathy prion protein variant PCR |
| url | http://www.jemb.bio.uaic.ro/index.php/jemb/article/view/91 |
| work_keys_str_mv | AT melihsercanustaoglu distributionofthe23bppolymorphismoftheprionproteingeneinjerseycattlesinturkey AT serbulentyigit distributionofthe23bppolymorphismoftheprionproteingeneinjerseycattlesinturkey AT muhammedtahakaya distributionofthe23bppolymorphismoftheprionproteingeneinjerseycattlesinturkey |