Changes in the Serum Metabolome in an Inflammatory Model of Osteoarthritis in Rats
Osteoarthritis (OA) is a pathology of great impact worldwide. Its physiopathology is not completely known, and it is usually diagnosed by imaging techniques performed at advanced stages of the disease. The aim of this study was to evaluate early serum metabolome changes and identify the main metabol...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/6/3158 |
| _version_ | 1797240729948389376 |
|---|---|
| author | Neus I. Berenguer Vicente J. Sifre Canet Carme Soler Canet Sergi Segarra Alejandra García de Carellán C. Iván Serra Aguado |
| author_facet | Neus I. Berenguer Vicente J. Sifre Canet Carme Soler Canet Sergi Segarra Alejandra García de Carellán C. Iván Serra Aguado |
| author_sort | Neus I. Berenguer |
| collection | DOAJ |
| description | Osteoarthritis (OA) is a pathology of great impact worldwide. Its physiopathology is not completely known, and it is usually diagnosed by imaging techniques performed at advanced stages of the disease. The aim of this study was to evaluate early serum metabolome changes and identify the main metabolites involved in an inflammatory OA animal model. This study was performed on thirty rats. OA was induced in all animals by intra-articular injection of monoiodoacetate into the knee joint. Blood samples were taken from all animals and analyzed by mass spectrometry before OA induction and 28, 56, and 84 days following induction. Histological evaluation confirmed OA in all samples. The results of this study allow the identification of several changes in 18 metabolites over time, including organic acids, benzenoids, heterocyclic compounds, and lipids after 28 days, organic acids after 56 days, and lipid classes after 84 days. We conclude that OA induces serological changes in the serum metabolome, which could serve as potential biomarkers. However, it was not possible to establish a relationship between the identified metabolites and the time at which the samples were taken. Therefore, these findings should be confirmed in future OA studies. |
| first_indexed | 2024-04-24T18:12:04Z |
| format | Article |
| id | doaj.art-2049674482014ee4b5c5e403dc6d207d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T18:12:04Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2049674482014ee4b5c5e403dc6d207d2024-03-27T13:45:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01256315810.3390/ijms25063158Changes in the Serum Metabolome in an Inflammatory Model of Osteoarthritis in RatsNeus I. Berenguer0Vicente J. Sifre Canet1Carme Soler Canet2Sergi Segarra3Alejandra García de Carellán4C. Iván Serra Aguado5Departamento de Medicina y Cirugía Animal, Universidad Católica de Valencia San Vicente Mártir, 46002 Valencia, SpainDepartamento de Medicina y Cirugía Animal, Universidad Católica de Valencia San Vicente Mártir, 46002 Valencia, SpainDepartamento de Medicina y Cirugía Animal, Universidad Católica de Valencia San Vicente Mártir, 46002 Valencia, SpainR&D Bioiberica SAU, Esplugues de Llobregat Barcelona, 08950 Barcelona, SpainDepartamento de Medicina y Cirugía Animal, Universidad Católica de Valencia San Vicente Mártir, 46002 Valencia, SpainDepartamento de Medicina y Cirugía Animal, Universidad Católica de Valencia San Vicente Mártir, 46002 Valencia, SpainOsteoarthritis (OA) is a pathology of great impact worldwide. Its physiopathology is not completely known, and it is usually diagnosed by imaging techniques performed at advanced stages of the disease. The aim of this study was to evaluate early serum metabolome changes and identify the main metabolites involved in an inflammatory OA animal model. This study was performed on thirty rats. OA was induced in all animals by intra-articular injection of monoiodoacetate into the knee joint. Blood samples were taken from all animals and analyzed by mass spectrometry before OA induction and 28, 56, and 84 days following induction. Histological evaluation confirmed OA in all samples. The results of this study allow the identification of several changes in 18 metabolites over time, including organic acids, benzenoids, heterocyclic compounds, and lipids after 28 days, organic acids after 56 days, and lipid classes after 84 days. We conclude that OA induces serological changes in the serum metabolome, which could serve as potential biomarkers. However, it was not possible to establish a relationship between the identified metabolites and the time at which the samples were taken. Therefore, these findings should be confirmed in future OA studies.https://www.mdpi.com/1422-0067/25/6/3158osteoarthritis (OA)metabolomicsliquid chromatography/mass spectrometry (LC/MS)metabolic pathwaylipid molecules |
| spellingShingle | Neus I. Berenguer Vicente J. Sifre Canet Carme Soler Canet Sergi Segarra Alejandra García de Carellán C. Iván Serra Aguado Changes in the Serum Metabolome in an Inflammatory Model of Osteoarthritis in Rats International Journal of Molecular Sciences osteoarthritis (OA) metabolomics liquid chromatography/mass spectrometry (LC/MS) metabolic pathway lipid molecules |
| title | Changes in the Serum Metabolome in an Inflammatory Model of Osteoarthritis in Rats |
| title_full | Changes in the Serum Metabolome in an Inflammatory Model of Osteoarthritis in Rats |
| title_fullStr | Changes in the Serum Metabolome in an Inflammatory Model of Osteoarthritis in Rats |
| title_full_unstemmed | Changes in the Serum Metabolome in an Inflammatory Model of Osteoarthritis in Rats |
| title_short | Changes in the Serum Metabolome in an Inflammatory Model of Osteoarthritis in Rats |
| title_sort | changes in the serum metabolome in an inflammatory model of osteoarthritis in rats |
| topic | osteoarthritis (OA) metabolomics liquid chromatography/mass spectrometry (LC/MS) metabolic pathway lipid molecules |
| url | https://www.mdpi.com/1422-0067/25/6/3158 |
| work_keys_str_mv | AT neusiberenguer changesintheserummetabolomeinaninflammatorymodelofosteoarthritisinrats AT vicentejsifrecanet changesintheserummetabolomeinaninflammatorymodelofosteoarthritisinrats AT carmesolercanet changesintheserummetabolomeinaninflammatorymodelofosteoarthritisinrats AT sergisegarra changesintheserummetabolomeinaninflammatorymodelofosteoarthritisinrats AT alejandragarciadecarellan changesintheserummetabolomeinaninflammatorymodelofosteoarthritisinrats AT civanserraaguado changesintheserummetabolomeinaninflammatorymodelofosteoarthritisinrats |