Genome-Wide Identification of PYL/RCAR ABA Receptors and Functional Analysis of <i>LbPYL10</i> in Heat Tolerance in Goji (<i>Lycium barbarum</i>)
The characterization of the PYL/RCAR ABA receptors in a great deal of plant species has dramatically advanced the study of ABA functions involved in key physiological processes. However, the genes in this family are still unclear in <i>Lycium</i> (Goji) plants, one of the well–known econ...
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MDPI AG
2024-03-01
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| Series: | Plants |
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| author | Zeyu Li Jiyao Liu Yan Chen Aihua Liang Wei He Xiaoya Qin Ken Qin Zixin Mu |
| author_facet | Zeyu Li Jiyao Liu Yan Chen Aihua Liang Wei He Xiaoya Qin Ken Qin Zixin Mu |
| author_sort | Zeyu Li |
| collection | DOAJ |
| description | The characterization of the PYL/RCAR ABA receptors in a great deal of plant species has dramatically advanced the study of ABA functions involved in key physiological processes. However, the genes in this family are still unclear in <i>Lycium</i> (Goji) plants, one of the well–known economically, medicinally, and ecologically valuable fruit crops. In the present work, 12 homologs of <i>Arabidopsis</i> PYL/RCAR ABA receptors were first identified and characterized from <i>Lycium</i> (L.) <i>barbarum</i> (LbPYLs). The quantitative real–time PCR (qRT–PCR) analysis showed that these genes had clear tissue–specific expression patterns, and most of them were transcribed in the root with the largest amount. Among the three subfamilies, while the Group I and Group III members were down–regulated by extraneous ABA, the Group II members were up–regulated. At 42 °C, most transcripts showed a rapid and violent up–regulation response to higher temperature, especially members of Group II. One of the genes in the Group II members, <i>LbPYL10</i>, was further functionally validated by virus–induced gene silencing (VIGS) technology. <i>LbPYL10</i> positively regulates heat stress tolerance in <i>L. barbarum</i> by alleviating chlorophyll degradation, thus maintaining chlorophyll stability. Integrating the endogenous ABA level increase following heat stress, it may be concluded that LbPYL–mediated ABA signaling plays a vital role in the thermotolerance of <i>L. barbarum</i> plants. Our results highlight the strong potential of <i>LbPYL</i> genes in breeding genetically modified <i>L. barbarum</i> crops that acclimate to climate change. |
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| last_indexed | 2024-04-24T17:53:33Z |
| publishDate | 2024-03-01 |
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| series | Plants |
| spelling | doaj.art-204d89e4e94b4378ae2df8f8a9f5006c2024-03-27T14:00:57ZengMDPI AGPlants2223-77472024-03-0113688710.3390/plants13060887Genome-Wide Identification of PYL/RCAR ABA Receptors and Functional Analysis of <i>LbPYL10</i> in Heat Tolerance in Goji (<i>Lycium barbarum</i>)Zeyu Li0Jiyao Liu1Yan Chen2Aihua Liang3Wei He4Xiaoya Qin5Ken Qin6Zixin Mu7College of Life Sciences, Northwest A&F University, Yangling 712100, ChinaCollege of Life Sciences, Northwest A&F University, Yangling 712100, ChinaCollege of Life Sciences, Northwest A&F University, Yangling 712100, ChinaCollege of Life Sciences & Technology, Tarim University, Alaer 843300, ChinaCollege of Life Sciences, Northwest A&F University, Yangling 712100, ChinaNational Wolfberry Engineering Research Center, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, ChinaNational Wolfberry Engineering Research Center, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, ChinaCollege of Life Sciences & Technology, Tarim University, Alaer 843300, ChinaThe characterization of the PYL/RCAR ABA receptors in a great deal of plant species has dramatically advanced the study of ABA functions involved in key physiological processes. However, the genes in this family are still unclear in <i>Lycium</i> (Goji) plants, one of the well–known economically, medicinally, and ecologically valuable fruit crops. In the present work, 12 homologs of <i>Arabidopsis</i> PYL/RCAR ABA receptors were first identified and characterized from <i>Lycium</i> (L.) <i>barbarum</i> (LbPYLs). The quantitative real–time PCR (qRT–PCR) analysis showed that these genes had clear tissue–specific expression patterns, and most of them were transcribed in the root with the largest amount. Among the three subfamilies, while the Group I and Group III members were down–regulated by extraneous ABA, the Group II members were up–regulated. At 42 °C, most transcripts showed a rapid and violent up–regulation response to higher temperature, especially members of Group II. One of the genes in the Group II members, <i>LbPYL10</i>, was further functionally validated by virus–induced gene silencing (VIGS) technology. <i>LbPYL10</i> positively regulates heat stress tolerance in <i>L. barbarum</i> by alleviating chlorophyll degradation, thus maintaining chlorophyll stability. Integrating the endogenous ABA level increase following heat stress, it may be concluded that LbPYL–mediated ABA signaling plays a vital role in the thermotolerance of <i>L. barbarum</i> plants. Our results highlight the strong potential of <i>LbPYL</i> genes in breeding genetically modified <i>L. barbarum</i> crops that acclimate to climate change.https://www.mdpi.com/2223-7747/13/6/887<i>Lycium barbarum</i>ABA signalingPYL/RCAR ABA receptorsgenome–wideheat stress |
| spellingShingle | Zeyu Li Jiyao Liu Yan Chen Aihua Liang Wei He Xiaoya Qin Ken Qin Zixin Mu Genome-Wide Identification of PYL/RCAR ABA Receptors and Functional Analysis of <i>LbPYL10</i> in Heat Tolerance in Goji (<i>Lycium barbarum</i>) Plants <i>Lycium barbarum</i> ABA signaling PYL/RCAR ABA receptors genome–wide heat stress |
| title | Genome-Wide Identification of PYL/RCAR ABA Receptors and Functional Analysis of <i>LbPYL10</i> in Heat Tolerance in Goji (<i>Lycium barbarum</i>) |
| title_full | Genome-Wide Identification of PYL/RCAR ABA Receptors and Functional Analysis of <i>LbPYL10</i> in Heat Tolerance in Goji (<i>Lycium barbarum</i>) |
| title_fullStr | Genome-Wide Identification of PYL/RCAR ABA Receptors and Functional Analysis of <i>LbPYL10</i> in Heat Tolerance in Goji (<i>Lycium barbarum</i>) |
| title_full_unstemmed | Genome-Wide Identification of PYL/RCAR ABA Receptors and Functional Analysis of <i>LbPYL10</i> in Heat Tolerance in Goji (<i>Lycium barbarum</i>) |
| title_short | Genome-Wide Identification of PYL/RCAR ABA Receptors and Functional Analysis of <i>LbPYL10</i> in Heat Tolerance in Goji (<i>Lycium barbarum</i>) |
| title_sort | genome wide identification of pyl rcar aba receptors and functional analysis of i lbpyl10 i in heat tolerance in goji i lycium barbarum i |
| topic | <i>Lycium barbarum</i> ABA signaling PYL/RCAR ABA receptors genome–wide heat stress |
| url | https://www.mdpi.com/2223-7747/13/6/887 |
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