Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro Study
The use of nanomaterials is becoming increasingly widespread, leading to substantial research focused on nanomedicine. Nevertheless, the lack of complete toxicity profiles limits nanomaterials’ uses, despite their remarkable diagnostic and therapeutic results on in vitro and in vivo models. Silver n...
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2021-05-01
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| Online Access: | https://www.mdpi.com/1996-1944/14/11/2792 |
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| author | Roberto Luna-Vázquez-Gómez María Evarista Arellano-García Juan Carlos García-Ramos Patricia Radilla-Chávez David Sergio Salas-Vargas Francisco Casillas-Figueroa Balam Ruiz-Ruiz Nina Bogdanchikova Alexey Pestryakov |
| author_facet | Roberto Luna-Vázquez-Gómez María Evarista Arellano-García Juan Carlos García-Ramos Patricia Radilla-Chávez David Sergio Salas-Vargas Francisco Casillas-Figueroa Balam Ruiz-Ruiz Nina Bogdanchikova Alexey Pestryakov |
| author_sort | Roberto Luna-Vázquez-Gómez |
| collection | DOAJ |
| description | The use of nanomaterials is becoming increasingly widespread, leading to substantial research focused on nanomedicine. Nevertheless, the lack of complete toxicity profiles limits nanomaterials’ uses, despite their remarkable diagnostic and therapeutic results on in vitro and in vivo models. Silver nanoparticles (AgNPs), particularly Argovit™, have shown microbicidal, virucidal, and antitumoral effects. Among the first-line toxicity tests is the hemolysis assay. Here, the hemolytic effect of Argovit™ AgNPs on erythrocytes from one healthy donor (HDE) and one diabetic donor (DDE) is evaluated by the hemolysis assay against AgNO<sub>3</sub>. The results showed that Argovit™, in concentrations ≤24 µg/mL of metallic silver, did not show a hemolytic effect on the HDE or DDE. On the contrary, AgNO<sub>3</sub> at the same concentration of silver ions produces more than 10% hemolysis in both the erythrocyte types. In all the experimental conditions assessed, the DDE was shown to be more prone to hemolysis than the HDE elicited by Ag<sup>+</sup> ions or AgNPs, but much more evident with Ag<sup>+</sup> ions. The results show that Argovit™ is the least hemolytic compared with the other twenty-two AgNP formulations previously reported, probably due to the polymer mass used to stabilize the Argovit™ formulation. The results obtained provide relevant information that contributes to obtaining a comprehensive toxicological profile to design safe and effective AgNP formulations. |
| first_indexed | 2024-03-10T11:05:05Z |
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| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T11:05:05Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-204d90c750a0421fbcf231e3322adcdd2023-11-21T21:09:41ZengMDPI AGMaterials1996-19442021-05-011411279210.3390/ma14112792Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro StudyRoberto Luna-Vázquez-Gómez0María Evarista Arellano-García1Juan Carlos García-Ramos2Patricia Radilla-Chávez3David Sergio Salas-Vargas4Francisco Casillas-Figueroa5Balam Ruiz-Ruiz6Nina Bogdanchikova7Alexey Pestryakov8Facultad de Ciencias, Universidad Autónoma de Baja California (UABC), Ensenada 22860, Baja California, MexicoFacultad de Ciencias, Universidad Autónoma de Baja California (UABC), Ensenada 22860, Baja California, MexicoEscuela de Ciencias de la Salud, Unidad Valle Dorado, Ensenada 22890, Baja California, MexicoEscuela de Ciencias de la Salud, Unidad Valle Dorado, Ensenada 22890, Baja California, MexicoEscuela de Ciencias de la Salud, Unidad Valle Dorado, Ensenada 22890, Baja California, MexicoFacultad de Ciencias, Universidad Autónoma de Baja California (UABC), Ensenada 22860, Baja California, MexicoDepartamento de Ciencias de la Salud, Unidad Regional Los Mochis, Universidad Autónoma de Occidente, Los Mochis 81223, Sinaloa, MexicoNanoscience and Nanotechnology Center (CNyN), National Autonomous University of Mexico (UNAM), Mexico City 58089, Distrito Federal, MexicoResearch School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, 634050 Tomsk, RussiaThe use of nanomaterials is becoming increasingly widespread, leading to substantial research focused on nanomedicine. Nevertheless, the lack of complete toxicity profiles limits nanomaterials’ uses, despite their remarkable diagnostic and therapeutic results on in vitro and in vivo models. Silver nanoparticles (AgNPs), particularly Argovit™, have shown microbicidal, virucidal, and antitumoral effects. Among the first-line toxicity tests is the hemolysis assay. Here, the hemolytic effect of Argovit™ AgNPs on erythrocytes from one healthy donor (HDE) and one diabetic donor (DDE) is evaluated by the hemolysis assay against AgNO<sub>3</sub>. The results showed that Argovit™, in concentrations ≤24 µg/mL of metallic silver, did not show a hemolytic effect on the HDE or DDE. On the contrary, AgNO<sub>3</sub> at the same concentration of silver ions produces more than 10% hemolysis in both the erythrocyte types. In all the experimental conditions assessed, the DDE was shown to be more prone to hemolysis than the HDE elicited by Ag<sup>+</sup> ions or AgNPs, but much more evident with Ag<sup>+</sup> ions. The results show that Argovit™ is the least hemolytic compared with the other twenty-two AgNP formulations previously reported, probably due to the polymer mass used to stabilize the Argovit™ formulation. The results obtained provide relevant information that contributes to obtaining a comprehensive toxicological profile to design safe and effective AgNP formulations.https://www.mdpi.com/1996-1944/14/11/2792in vitro hemolysissilver nanoparticlesdiabetic erythrocytes |
| spellingShingle | Roberto Luna-Vázquez-Gómez María Evarista Arellano-García Juan Carlos García-Ramos Patricia Radilla-Chávez David Sergio Salas-Vargas Francisco Casillas-Figueroa Balam Ruiz-Ruiz Nina Bogdanchikova Alexey Pestryakov Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro Study Materials in vitro hemolysis silver nanoparticles diabetic erythrocytes |
| title | Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro Study |
| title_full | Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro Study |
| title_fullStr | Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro Study |
| title_full_unstemmed | Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro Study |
| title_short | Hemolysis of Human Erythrocytes by Argovit™ AgNPs from Healthy and Diabetic Donors: An In Vitro Study |
| title_sort | hemolysis of human erythrocytes by argovit™ agnps from healthy and diabetic donors an in vitro study |
| topic | in vitro hemolysis silver nanoparticles diabetic erythrocytes |
| url | https://www.mdpi.com/1996-1944/14/11/2792 |
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