2′-19F labelling of ribose in RNAs: a tool to analyse RNA/protein interactions by NMR in physiological conditions

2′-19F labelling of ribose in RNAs: a tool to analyse RNA/protein interactions by NMR in physiological conditions

Protein-RNA interactions are central to numerous cellular processes. In this work, we present an easy and straightforward NMR-based approach to determine the RNA binding site of RNA binding proteins and to evaluate the binding of pairs of proteins to a single-stranded RNA (ssRNA) under physiological...

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Bibliographic Details
Main Authors: Hesna Kara, Alexander Axer, Frederick W. Muskett, Carlos J. Bueno-Alejo, Vasileios Paschalis, Andrea Taladriz-Sender, Sumera Tubasum, Marina Santana Vega, Zhengyun Zhao, Alasdair W. Clark, Andrew J. Hudson, Ian C. Eperon, Glenn A. Burley, Cyril Dominguez
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-02-01
Series:Frontiers in Molecular Biosciences
Subjects:
RNA-protein interaction
19F NMR spectroscopy
RNA binding proteins
RNA labelling
concurrent/competitive binding
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2024.1325041/full

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