Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials
Digestibility trials need a viable rumen fluid as <i>inoculum</i> to degrade feeds. The variability of rumen fluid depends on the animal’s diet, while its viability is greatly influenced by the sampling and handling procedures. In this article, we present a replicable protocol for sampli...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/5/4/59 |
| _version_ | 1797443061890940928 |
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| author | Riccardo Fortina Sara Glorio Patrucco Salvatore Barbera Sonia Tassone |
| author_facet | Riccardo Fortina Sara Glorio Patrucco Salvatore Barbera Sonia Tassone |
| author_sort | Riccardo Fortina |
| collection | DOAJ |
| description | Digestibility trials need a viable rumen fluid as <i>inoculum</i> to degrade feeds. The variability of rumen fluid depends on the animal’s diet, while its viability is greatly influenced by the sampling and handling procedures. In this article, we present a replicable protocol for sampling the rumen fluid from slaughtered animals for <i>in vitro</i> digestibility trials. A detailed list of the tools and a step-by-step standardized procedure for the collection, storage and the transportation of the rumen fluid from the slaughterhouse to the laboratory is presented. We also describe a digestibility trial for establishing the maximum storage time of rumen fluid from sampling to its use. The results show that the rumen fluid, collected and maintained according to the proposed protocol, can be stored and used from 30 to 300 min from sampling without significantly compromising the fermentative activity of the microbial population. |
| first_indexed | 2024-03-09T12:50:41Z |
| format | Article |
| id | doaj.art-2052936b70a2457eb98d9753e81916dc |
| institution | Directory Open Access Journal |
| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-03-09T12:50:41Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj.art-2052936b70a2457eb98d9753e81916dc2023-11-30T22:06:05ZengMDPI AGMethods and Protocols2409-92792022-07-01545910.3390/mps5040059Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility TrialsRiccardo Fortina0Sara Glorio Patrucco1Salvatore Barbera2Sonia Tassone3Department of Agriculture, Forest and Food Sciences, University of Turin, 10095 Grugliasco, ItalyDepartment of Agriculture, Forest and Food Sciences, University of Turin, 10095 Grugliasco, ItalyDepartment of Agriculture, Forest and Food Sciences, University of Turin, 10095 Grugliasco, ItalyDepartment of Agriculture, Forest and Food Sciences, University of Turin, 10095 Grugliasco, ItalyDigestibility trials need a viable rumen fluid as <i>inoculum</i> to degrade feeds. The variability of rumen fluid depends on the animal’s diet, while its viability is greatly influenced by the sampling and handling procedures. In this article, we present a replicable protocol for sampling the rumen fluid from slaughtered animals for <i>in vitro</i> digestibility trials. A detailed list of the tools and a step-by-step standardized procedure for the collection, storage and the transportation of the rumen fluid from the slaughterhouse to the laboratory is presented. We also describe a digestibility trial for establishing the maximum storage time of rumen fluid from sampling to its use. The results show that the rumen fluid, collected and maintained according to the proposed protocol, can be stored and used from 30 to 300 min from sampling without significantly compromising the fermentative activity of the microbial population.https://www.mdpi.com/2409-9279/5/4/59rumen fluid collectionslaughtered ruminantsrumen fluid storage<i>in vitro</i> digestibility |
| spellingShingle | Riccardo Fortina Sara Glorio Patrucco Salvatore Barbera Sonia Tassone Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials Methods and Protocols rumen fluid collection slaughtered ruminants rumen fluid storage <i>in vitro</i> digestibility |
| title | Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials |
| title_full | Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials |
| title_fullStr | Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials |
| title_full_unstemmed | Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials |
| title_short | Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials |
| title_sort | rumen fluid from slaughtered animals a standardized procedure for sampling storage and use in digestibility trials |
| topic | rumen fluid collection slaughtered ruminants rumen fluid storage <i>in vitro</i> digestibility |
| url | https://www.mdpi.com/2409-9279/5/4/59 |
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